Tracing Low‐CO2 Fluxes in Soil Incubation and 13C Labeling Experiments: A Simplified Gas Sampling System for Respiration and Photosynthesis Measurements.
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| Title: | Tracing Low‐CO |
|---|---|
| Authors: | Witzgall, K.1 (AUTHOR) kristina.witzgall@tum.de, Hesse, B. D.2 (AUTHOR), Seguel, O.3 (AUTHOR), Oses, R.4 (AUTHOR), Grams, T. E. E.2 (AUTHOR), Mueller, C. W.1,5 (AUTHOR) |
| Source: | Journal of Geophysical Research. Biogeosciences. Sep2023, Vol. 128 Issue 9, p1-11. 11p. |
| Subject Terms: | *Soil air, *Arid soils, *Carbon cycle, Respiratory measurements, Soil respiration |
| Abstract: | Quantifying carbon dioxide (CO2) fluxes between soil and atmosphere is key in understanding net ecosystem C exchange and biogeochemical C cycling in plant‐soil systems. In ecosystems with low primary production and sparse vegetation, for example, dry lands or subpolar regions where C fluxes are small, measurement sensitivity is key—even so when measurements are combined with isotopic labeling. Here, we present a simplified gas sampling system developed to facilitate sampling and measurement of low soil CO2 fluxes as well as in situ 13CO2 labeling in the same setup. The capacity of the system was tested in a set of feature tests along with gas measurements of dryland soil‐biocrust systems. The system's sensitivity to capture minor changes in CO2 concentration was confirmed in respiration and photosynthesis measurements of soil‐biocrust systems, where fluxes down to 0.1 μmol CO2 m−2 s−1 were quantified. A balloon, implemented to counterbalance underpressure build‐up during gas withdrawal, mitigated 72% of pressure differences at sampling. The overall system volume was reduced to a minimum to limit contamination caused by residual air, and the design enabled one‐step flushing and evacuation of system compartments and gas sample bags, successfully ruling out cross‐contamination between samples. Ultimately, this system offers a flexible and accessible solution for CO2 measurements that can be applied not only on arid soils with low biological activity and turnover rates, but also on plant‐soil systems. The modifications enabled larger, and thereby more representative, sample volumes to be collected while limiting incubation, contamination, and pressure effects on the intact soil system. Plain Language Summary: Measurements of carbon dioxide (CO2) fluxes between soil and atmosphere are crucial to understand the terrestrial carbon cycle. In dry regions, these fluxes are rather small as both the release of CO2 via microbial respiration and uptake of CO2 via photosynthesis is limited by lack of water and sparse vegetation cover. We have developed a simplified CO2 gas sampling setup suitable for incubation experiments of soils with low biological activity and turnover rates, for example, soils from dry lands or subpolar regions, using only cost‐effective, easily available and replaceable system compartments. We tested system parameters in a set of feature tests and could rule out leakage and cross‐contamination. We further confirmed the suitability of the system for capturing small CO2 fluxes in photosynthesis and respiration measurements of soils with biocrusts. A balloon was installed to counterbalance pressure build‐up during sampling, allowing for larger gas volumes to be collected, which resulted in a mitigation of 72% of the underpressure build‐up. This system offers a straightforward and accessible solution for CO2 measurements, with features limiting contamination and pressure effects on the research sample. Key Points: We present a simplified and low‐cost gas sampling system, developed for quantifying CO2 fluxes in soil incubation experimentsRespiration rates down to 0.1 μmol CO2 m−2 s−1 were quantified, confirming the suitability of the system for low‐CO2 flux measurementsA balloon was installed, mitigating 72% of pressure changes in the headspace during sampling [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Geophysical Research. Biogeosciences is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 172367439 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Tracing Low‐CO<subscript>2</subscript> Fluxes in Soil Incubation and <superscript>13</superscript>C Labeling Experiments: A Simplified Gas Sampling System for Respiration and Photosynthesis Measurements. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Witzgall%2C+K%2E%22">Witzgall, K.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> kristina.witzgall@tum.de</i><br /><searchLink fieldCode="AR" term="%22Hesse%2C+B%2E+D%2E%22">Hesse, B. D.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Seguel%2C+O%2E%22">Seguel, O.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Oses%2C+R%2E%22">Oses, R.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Grams%2C+T%2E+E%2E+E%2E%22">Grams, T. E. E.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mueller%2C+C%2E+W%2E%22">Mueller, C. W.</searchLink><relatesTo>1,5</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Geophysical+Research%2E+Biogeosciences%22">Journal of Geophysical Research. Biogeosciences</searchLink>. Sep2023, Vol. 128 Issue 9, p1-11. 11p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Soil+air%22">Soil air</searchLink><br />*<searchLink fieldCode="DE" term="%22Arid+soils%22">Arid soils</searchLink><br />*<searchLink fieldCode="DE" term="%22Carbon+cycle%22">Carbon cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Respiratory+measurements%22">Respiratory measurements</searchLink><br /><searchLink fieldCode="DE" term="%22Soil+respiration%22">Soil respiration</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Quantifying carbon dioxide (CO2) fluxes between soil and atmosphere is key in understanding net ecosystem C exchange and biogeochemical C cycling in plant‐soil systems. In ecosystems with low primary production and sparse vegetation, for example, dry lands or subpolar regions where C fluxes are small, measurement sensitivity is key—even so when measurements are combined with isotopic labeling. Here, we present a simplified gas sampling system developed to facilitate sampling and measurement of low soil CO2 fluxes as well as in situ 13CO2 labeling in the same setup. The capacity of the system was tested in a set of feature tests along with gas measurements of dryland soil‐biocrust systems. The system's sensitivity to capture minor changes in CO2 concentration was confirmed in respiration and photosynthesis measurements of soil‐biocrust systems, where fluxes down to 0.1 μmol CO2 m−2 s−1 were quantified. A balloon, implemented to counterbalance underpressure build‐up during gas withdrawal, mitigated 72% of pressure differences at sampling. The overall system volume was reduced to a minimum to limit contamination caused by residual air, and the design enabled one‐step flushing and evacuation of system compartments and gas sample bags, successfully ruling out cross‐contamination between samples. Ultimately, this system offers a flexible and accessible solution for CO2 measurements that can be applied not only on arid soils with low biological activity and turnover rates, but also on plant‐soil systems. The modifications enabled larger, and thereby more representative, sample volumes to be collected while limiting incubation, contamination, and pressure effects on the intact soil system. Plain Language Summary: Measurements of carbon dioxide (CO2) fluxes between soil and atmosphere are crucial to understand the terrestrial carbon cycle. In dry regions, these fluxes are rather small as both the release of CO2 via microbial respiration and uptake of CO2 via photosynthesis is limited by lack of water and sparse vegetation cover. We have developed a simplified CO2 gas sampling setup suitable for incubation experiments of soils with low biological activity and turnover rates, for example, soils from dry lands or subpolar regions, using only cost‐effective, easily available and replaceable system compartments. We tested system parameters in a set of feature tests and could rule out leakage and cross‐contamination. We further confirmed the suitability of the system for capturing small CO2 fluxes in photosynthesis and respiration measurements of soils with biocrusts. A balloon was installed to counterbalance pressure build‐up during sampling, allowing for larger gas volumes to be collected, which resulted in a mitigation of 72% of the underpressure build‐up. This system offers a straightforward and accessible solution for CO2 measurements, with features limiting contamination and pressure effects on the research sample. Key Points: We present a simplified and low‐cost gas sampling system, developed for quantifying CO2 fluxes in soil incubation experimentsRespiration rates down to 0.1 μmol CO2 m−2 s−1 were quantified, confirming the suitability of the system for low‐CO2 flux measurementsA balloon was installed, mitigating 72% of pressure changes in the headspace during sampling [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Geophysical Research. Biogeosciences is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1029/2023JG007410 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1 Subjects: – SubjectFull: Soil air Type: general – SubjectFull: Arid soils Type: general – SubjectFull: Carbon cycle Type: general – SubjectFull: Respiratory measurements Type: general – SubjectFull: Soil respiration Type: general Titles: – TitleFull: Tracing Low‐CO2 Fluxes in Soil Incubation and 13C Labeling Experiments: A Simplified Gas Sampling System for Respiration and Photosynthesis Measurements. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Witzgall, K. – PersonEntity: Name: NameFull: Hesse, B. D. – PersonEntity: Name: NameFull: Seguel, O. – PersonEntity: Name: NameFull: Oses, R. – PersonEntity: Name: NameFull: Grams, T. E. E. – PersonEntity: Name: NameFull: Mueller, C. W. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 21698953 Numbering: – Type: volume Value: 128 – Type: issue Value: 9 Titles: – TitleFull: Journal of Geophysical Research. Biogeosciences Type: main |
| ResultId | 1 |
