The dissemination of antibiotic resistance genes and heavy metal resistance genes along the sewage pipe under Cu and Zn stress.
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| Title: | The dissemination of antibiotic resistance genes and heavy metal resistance genes along the sewage pipe under Cu and Zn stress. |
|---|---|
| Authors: | Wu, Xin1 (AUTHOR), Liu, Kunyu1 (AUTHOR), Chen, Aoxi1 (AUTHOR), Lu, Yi1 (AUTHOR), Qi, Tianle1 (AUTHOR), Lv, Zhi1 (AUTHOR), E, Quanjie1 (AUTHOR), Wei, Haodong1 (AUTHOR), Liu, Cuiyun1,2 (AUTHOR) yunduobai@126.com |
| Source: | Journal of Environmental Sciences (Elsevier). Jun2026, Vol. 164, p371-381. 11p. |
| Subject Terms: | *Microbial communities, Horizontal gene transfer, Drug resistance in bacteria, Biofilms, Sewer pipes |
| Abstract: | • Cu and Zn exposure changed the microbial communities in sewage pipe. • Cu and Zn promoted horizontal gene transfer by co-resistance and cross-resistance. • At the front more tryptophan and humic acid was secreted to improve scour resistance. • At higher Cu and Zn concentration, abundance of most genes at the front was higher. • At higher Cu and Zn concentration, major potential hosts at the front were abundant. The long-term existence of heavy metals in sewage pipe was one of the selective pressures of resistance genes. This work explored the dissemination mechanisms of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) under Cu and Zn stress and their dissemination differences at different locations along the pipe. It was found that at lower Cu and Zn concentrations, microorganisms secreted more extracellular polymeric substance (EPS) for self-protection, with a 77.83 % increase on the 14th day. As the concentrations increased, the relative abundance of most HMRGs increased. TnpA and intl1 showed positive correlations with major ARGs and HMRGs. Moreover, the potential hosts of efflux pump genes (copB, tetA and czcA) were resistant to Cu and Zn. It could be inferred that the co-resistance and cross-resistance induced by heavy metals promote the horizontal gene transfer. When Cu and Zn concentrations were higher, the combined stress exceeded the impact of water flow, becoming primary external environmental pressure. The differences of EPS, protein and polysaccharide contents became smaller among the front, middle and posterior of pipe. While at the front, the microorganisms were more abundant, and the relative abundance of most resistance genes were higher. Correspondingly, the relative abundance of major potential hosts (e.g., unclassified_f_Bacteroidetes_vadinHA17, Smithella, Caldisericum) at the front sediment was significantly higher than that of the posterior (p ≤ 0.05). The study revealed the dissemination law of genes in sewage pipe under Cu and Zn stress, and laid theoretical foundations for further research on blocking the dissemination of genes. [Display omitted] [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Environmental Sciences (Elsevier) is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | GreenFILE |
| FullText | Text: Availability: 0 |
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| Header | DbId: 8gh DbLabel: GreenFILE An: 193395090 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The dissemination of antibiotic resistance genes and heavy metal resistance genes along the sewage pipe under Cu and Zn stress. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wu%2C+Xin%22">Wu, Xin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Kunyu%22">Liu, Kunyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Aoxi%22">Chen, Aoxi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lu%2C+Yi%22">Lu, Yi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qi%2C+Tianle%22">Qi, Tianle</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lv%2C+Zhi%22">Lv, Zhi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22E%2C+Quanjie%22">E, Quanjie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wei%2C+Haodong%22">Wei, Haodong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Cuiyun%22">Liu, Cuiyun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> yunduobai@126.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Environmental+Sciences+%28Elsevier%29%22">Journal of Environmental Sciences (Elsevier)</searchLink>. Jun2026, Vol. 164, p371-381. 11p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Microbial+communities%22">Microbial communities</searchLink><br /><searchLink fieldCode="DE" term="%22Horizontal+gene+transfer%22">Horizontal gene transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+resistance+in+bacteria%22">Drug resistance in bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink><br /><searchLink fieldCode="DE" term="%22Sewer+pipes%22">Sewer pipes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • Cu and Zn exposure changed the microbial communities in sewage pipe. • Cu and Zn promoted horizontal gene transfer by co-resistance and cross-resistance. • At the front more tryptophan and humic acid was secreted to improve scour resistance. • At higher Cu and Zn concentration, abundance of most genes at the front was higher. • At higher Cu and Zn concentration, major potential hosts at the front were abundant. The long-term existence of heavy metals in sewage pipe was one of the selective pressures of resistance genes. This work explored the dissemination mechanisms of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) under Cu and Zn stress and their dissemination differences at different locations along the pipe. It was found that at lower Cu and Zn concentrations, microorganisms secreted more extracellular polymeric substance (EPS) for self-protection, with a 77.83 % increase on the 14th day. As the concentrations increased, the relative abundance of most HMRGs increased. TnpA and intl1 showed positive correlations with major ARGs and HMRGs. Moreover, the potential hosts of efflux pump genes (copB, tetA and czcA) were resistant to Cu and Zn. It could be inferred that the co-resistance and cross-resistance induced by heavy metals promote the horizontal gene transfer. When Cu and Zn concentrations were higher, the combined stress exceeded the impact of water flow, becoming primary external environmental pressure. The differences of EPS, protein and polysaccharide contents became smaller among the front, middle and posterior of pipe. While at the front, the microorganisms were more abundant, and the relative abundance of most resistance genes were higher. Correspondingly, the relative abundance of major potential hosts (e.g., unclassified_f_Bacteroidetes_vadinHA17, Smithella, Caldisericum) at the front sediment was significantly higher than that of the posterior (p ≤ 0.05). The study revealed the dissemination law of genes in sewage pipe under Cu and Zn stress, and laid theoretical foundations for further research on blocking the dissemination of genes. [Display omitted] [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Environmental Sciences (Elsevier) is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jes.2025.08.031 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 371 Subjects: – SubjectFull: Microbial communities Type: general – SubjectFull: Horizontal gene transfer Type: general – SubjectFull: Drug resistance in bacteria Type: general – SubjectFull: Biofilms Type: general – SubjectFull: Sewer pipes Type: general Titles: – TitleFull: The dissemination of antibiotic resistance genes and heavy metal resistance genes along the sewage pipe under Cu and Zn stress. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wu, Xin – PersonEntity: Name: NameFull: Liu, Kunyu – PersonEntity: Name: NameFull: Chen, Aoxi – PersonEntity: Name: NameFull: Lu, Yi – PersonEntity: Name: NameFull: Qi, Tianle – PersonEntity: Name: NameFull: Lv, Zhi – PersonEntity: Name: NameFull: E, Quanjie – PersonEntity: Name: NameFull: Wei, Haodong – PersonEntity: Name: NameFull: Liu, Cuiyun IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 10010742 Numbering: – Type: volume Value: 164 Titles: – TitleFull: Journal of Environmental Sciences (Elsevier) Type: main |
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