View in EDS

Novel hyperpolarizable and fluorescent dyes in lipid membranes: studying membrane potentials using nonlinear optical and fluorescence

Saved in:
Bibliographic Details
Title: Novel hyperpolarizable and fluorescent dyes in lipid membranes: studying membrane potentials using nonlinear optical and fluorescence
Authors: Bechem, M.1, Beutner, S.2, Burkhardt, N.1, Fuchs, C.2, Kryschi, C.3, Paffhausen, W.1, Reiffers, B.2, Schade, A.1, Schlue, W.-R.4, Schmid, D.5, Schneider, L.2, Schulte, P.4, Wimmer, T.5, Witzak, D.2, Martin, H.-D.2 martin@uni-duesseldorf.de
Source: Electrochimica Acta. Sep2003, Vol. 48 Issue 20-22, p3387. 7p.
Subjects: Energy transfer, Fluorescence, Bilayer lipid membranes
Abstract: Potentiometric optical probes gain importance. Here, we report on the application of those probes to study transmembrane potentials using nonlinear optical (NLO) and fluorescence techniques. A NLO spectroscopy method is presented which allows for precise recording of membrane potential changes. To this end the signal intensity of second harmonic generation (SHG) mediated by novel acetylenic hemicyanine dye molecules embedded in the membrane of Retzius neuron cells was used. Bichromophoric systems have been designed for efficient energy transfer measurements. In many cells these bichromophores change their behaviour from stationary fast-response dyes to slow-response redistribution dyes. Completely novel potentiometric probes acting in a different and most efficient way were constructed using one aminostyrylpyridinium (ASP) bichromophoric dye and one oxonol dye. The redistribution ability of the ASP dye can be optimised by controlled and tailor-made modification of its substituents. In combination with the anionic oxonol dye DiBAC4(3) a bichromophoric system results with two chromophores showing opposite redistribution behaviour. Fluorescence ratio changes of 100% for a 100 mV depolarization can be recorded. [Copyright &y& Elsevier]
Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 10568576
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Novel hyperpolarizable and fluorescent dyes in lipid membranes: studying membrane potentials using nonlinear optical and fluorescence
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Bechem%2C+M%2E%22">Bechem, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Beutner%2C+S%2E%22">Beutner, S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Burkhardt%2C+N%2E%22">Burkhardt, N.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fuchs%2C+C%2E%22">Fuchs, C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kryschi%2C+C%2E%22">Kryschi, C.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Paffhausen%2C+W%2E%22">Paffhausen, W.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Reiffers%2C+B%2E%22">Reiffers, B.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Schade%2C+A%2E%22">Schade, A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Schlue%2C+W%2E-R%2E%22">Schlue, W.-R.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Schmid%2C+D%2E%22">Schmid, D.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Schneider%2C+L%2E%22">Schneider, L.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Schulte%2C+P%2E%22">Schulte, P.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Wimmer%2C+T%2E%22">Wimmer, T.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Witzak%2C+D%2E%22">Witzak, D.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Martin%2C+H%2E-D%2E%22">Martin, H.-D.</searchLink><relatesTo>2</relatesTo><i> martin@uni-duesseldorf.de</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Electrochimica+Acta%22">Electrochimica Acta</searchLink>. Sep2003, Vol. 48 Issue 20-22, p3387. 7p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Energy+transfer%22">Energy transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Bilayer+lipid+membranes%22">Bilayer lipid membranes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Potentiometric optical probes gain importance. Here, we report on the application of those probes to study transmembrane potentials using nonlinear optical (NLO) and fluorescence techniques. A NLO spectroscopy method is presented which allows for precise recording of membrane potential changes. To this end the signal intensity of second harmonic generation (SHG) mediated by novel acetylenic hemicyanine dye molecules embedded in the membrane of Retzius neuron cells was used. Bichromophoric systems have been designed for efficient energy transfer measurements. In many cells these bichromophores change their behaviour from stationary fast-response dyes to slow-response redistribution dyes. Completely novel potentiometric probes acting in a different and most efficient way were constructed using one aminostyrylpyridinium (ASP) bichromophoric dye and one oxonol dye. The redistribution ability of the ASP dye can be optimised by controlled and tailor-made modification of its substituents. In combination with the anionic oxonol dye DiBAC4(3) a bichromophoric system results with two chromophores showing opposite redistribution behaviour. Fluorescence ratio changes of 100% for a 100 mV depolarization can be recorded. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Electrochimica Acta is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=10568576
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/S0013-4686(03)00409-2
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 3387
    Subjects:
      – SubjectFull: Energy transfer
        Type: general
      – SubjectFull: Fluorescence
        Type: general
      – SubjectFull: Bilayer lipid membranes
        Type: general
    Titles:
      – TitleFull: Novel hyperpolarizable and fluorescent dyes in lipid membranes: studying membrane potentials using nonlinear optical and fluorescence
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Bechem, M.
      – PersonEntity:
          Name:
            NameFull: Beutner, S.
      – PersonEntity:
          Name:
            NameFull: Burkhardt, N.
      – PersonEntity:
          Name:
            NameFull: Fuchs, C.
      – PersonEntity:
          Name:
            NameFull: Kryschi, C.
      – PersonEntity:
          Name:
            NameFull: Paffhausen, W.
      – PersonEntity:
          Name:
            NameFull: Reiffers, B.
      – PersonEntity:
          Name:
            NameFull: Schade, A.
      – PersonEntity:
          Name:
            NameFull: Schlue, W.-R.
      – PersonEntity:
          Name:
            NameFull: Schmid, D.
      – PersonEntity:
          Name:
            NameFull: Schneider, L.
      – PersonEntity:
          Name:
            NameFull: Schulte, P.
      – PersonEntity:
          Name:
            NameFull: Wimmer, T.
      – PersonEntity:
          Name:
            NameFull: Witzak, D.
      – PersonEntity:
          Name:
            NameFull: Martin, H.-D.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 30
              M: 09
              Text: Sep2003
              Type: published
              Y: 2003
          Identifiers:
            – Type: issn-print
              Value: 00134686
          Numbering:
            – Type: volume
              Value: 48
            – Type: issue
              Value: 20-22
          Titles:
            – TitleFull: Electrochimica Acta
              Type: main
ResultId 1