Pressure versus pH phase diagrams of two lysozymes crystallized in agarose gel.
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| Title: | Pressure versus pH phase diagrams of two lysozymes crystallized in agarose gel. |
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| Authors: | Kadri, A.1,2,3 (AUTHOR), Damak, M.3 (AUTHOR), Lorber, B.1 (AUTHOR) B.Lorber@ibmc.u-strasbg.fr, Giegé, R.1 (AUTHOR), Jenner, G.2 (AUTHOR) |
| Source: | High Pressure Research. Dec2003, Vol. 23 Issue 4, p485-491. 7p. |
| Subjects: | Crystallization, Lysozymes, Proteins, Pressure, Phase diagrams |
| Abstract: | The effect of hydrostatic pressure and pH on the crystallization of tetragonal hen lysozyme crystals (HeL, M r 14,300) and hexagonal turkey lysozyme crystals (TeL, M r 14,200) in agarose gel was studied. Samples (adjusted to a pH in the range 4-6) were pressurized at 0.1-100 MPa for nine days at 293 K. The morphology and number of crystals as well as protein solubility were analyzed after depressurization. For both proteins and whatever the pH, a higher pressure resulted in greater numbers of crystals and greater solubility values. At any pressure, the solubility and number of crystals were lower at the highest pH. Thus the physical chemical parameters and the outcome of the crystallization process are more affected by pressure at pH 4.0 than at pH 6.0. In the case of HeL, either high pressure or high pH induces a transition from the initial tetragonal form to an orthorhombic one. The observed effects are related to minor differences in the macromolecular structures. [ABSTRACT FROM AUTHOR] |
| Copyright of High Pressure Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 11623182 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Pressure versus pH phase diagrams of two lysozymes crystallized in agarose gel. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kadri%2C+A%2E%22">Kadri, A.</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Damak%2C+M%2E%22">Damak, M.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lorber%2C+B%2E%22">Lorber, B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> B.Lorber@ibmc.u-strasbg.fr</i><br /><searchLink fieldCode="AR" term="%22Giegé%2C+R%2E%22">Giegé, R.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jenner%2C+G%2E%22">Jenner, G.</searchLink><relatesTo>2</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22High+Pressure+Research%22">High Pressure Research</searchLink>. Dec2003, Vol. 23 Issue 4, p485-491. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Crystallization%22">Crystallization</searchLink><br /><searchLink fieldCode="DE" term="%22Lysozymes%22">Lysozymes</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Pressure%22">Pressure</searchLink><br /><searchLink fieldCode="DE" term="%22Phase+diagrams%22">Phase diagrams</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The effect of hydrostatic pressure and pH on the crystallization of tetragonal hen lysozyme crystals (HeL, M r 14,300) and hexagonal turkey lysozyme crystals (TeL, M r 14,200) in agarose gel was studied. Samples (adjusted to a pH in the range 4-6) were pressurized at 0.1-100 MPa for nine days at 293 K. The morphology and number of crystals as well as protein solubility were analyzed after depressurization. For both proteins and whatever the pH, a higher pressure resulted in greater numbers of crystals and greater solubility values. At any pressure, the solubility and number of crystals were lower at the highest pH. Thus the physical chemical parameters and the outcome of the crystallization process are more affected by pressure at pH 4.0 than at pH 6.0. In the case of HeL, either high pressure or high pH induces a transition from the initial tetragonal form to an orthorhombic one. The observed effects are related to minor differences in the macromolecular structures. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of High Pressure Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=11623182 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/08957950310001621030 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 485 Subjects: – SubjectFull: Crystallization Type: general – SubjectFull: Lysozymes Type: general – SubjectFull: Proteins Type: general – SubjectFull: Pressure Type: general – SubjectFull: Phase diagrams Type: general Titles: – TitleFull: Pressure versus pH phase diagrams of two lysozymes crystallized in agarose gel. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kadri, A. – PersonEntity: Name: NameFull: Damak, M. – PersonEntity: Name: NameFull: Lorber, B. – PersonEntity: Name: NameFull: Giegé, R. – PersonEntity: Name: NameFull: Jenner, G. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 08957959 Numbering: – Type: volume Value: 23 – Type: issue Value: 4 Titles: – TitleFull: High Pressure Research Type: main |
| ResultId | 1 |
