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Analytical figures of merit for a novel tissue imaging system.

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Title: Analytical figures of merit for a novel tissue imaging system.
Authors: Straus, R. N.1, Carew, A.1, Sandkuijl, D.1, Closson, T.1, Baranov, V. I.1, Loboda, A.1
Source: JAAS (Journal of Analytical Atomic Spectrometry). May2017, Vol. 32 Issue 5, p1044-1051. 8p.
Subjects: Imaging systems in biology, Laser ablation, Cytometry, Polymethylmethacrylate, Pixels
Abstract: A novel laser-ablation sample introduction system has been developed for the Fluidigm® Helios™ mass cytometer to enable highly multiplexed imaging of biological samples. Herein, we report on the analytical figures of merit for this system using a slide, coated uniformly with a metal-doped PMMA film, as a test sample. The system is based on the use of a fifth harmonic Nd–YAG laser focused to a spot size of 1 micrometer or less. A fast wash-out ablation chamber provides resolution of individual plumes when operated at a 100 Hz or 200 Hz laser repetition frequency. The system exhibits a linear response to metal concentration in the sample over 4 orders of magnitude. The variation of signals generated for 1 micrometer diameter pixels is ≤15% RSD. Pixel-to-pixel sample contamination due to redeposition of the ablated material is approximately 0.6%. The variation of the ablated crater diameter with laser energy is also explored, demonstrating a linear behaviour over a limited range of laser energies. [ABSTRACT FROM AUTHOR]
Copyright of JAAS (Journal of Analytical Atomic Spectrometry) is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 122973213
AccessLevel: 6
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PubTypeId: academicJournal
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  Data: Analytical figures of merit for a novel tissue imaging system.
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  Data: <searchLink fieldCode="JN" term="%22JAAS+%28Journal+of+Analytical+Atomic+Spectrometry%29%22">JAAS (Journal of Analytical Atomic Spectrometry)</searchLink>. May2017, Vol. 32 Issue 5, p1044-1051. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Imaging+systems+in+biology%22">Imaging systems in biology</searchLink><br /><searchLink fieldCode="DE" term="%22Laser+ablation%22">Laser ablation</searchLink><br /><searchLink fieldCode="DE" term="%22Cytometry%22">Cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Polymethylmethacrylate%22">Polymethylmethacrylate</searchLink><br /><searchLink fieldCode="DE" term="%22Pixels%22">Pixels</searchLink>
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  Label: Abstract
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  Data: A novel laser-ablation sample introduction system has been developed for the Fluidigm® Helios™ mass cytometer to enable highly multiplexed imaging of biological samples. Herein, we report on the analytical figures of merit for this system using a slide, coated uniformly with a metal-doped PMMA film, as a test sample. The system is based on the use of a fifth harmonic Nd–YAG laser focused to a spot size of 1 micrometer or less. A fast wash-out ablation chamber provides resolution of individual plumes when operated at a 100 Hz or 200 Hz laser repetition frequency. The system exhibits a linear response to metal concentration in the sample over 4 orders of magnitude. The variation of signals generated for 1 micrometer diameter pixels is ≤15% RSD. Pixel-to-pixel sample contamination due to redeposition of the ablated material is approximately 0.6%. The variation of the ablated crater diameter with laser energy is also explored, demonstrating a linear behaviour over a limited range of laser energies. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of JAAS (Journal of Analytical Atomic Spectrometry) is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1039/c6ja00420b
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 1044
    Subjects:
      – SubjectFull: Imaging systems in biology
        Type: general
      – SubjectFull: Laser ablation
        Type: general
      – SubjectFull: Cytometry
        Type: general
      – SubjectFull: Polymethylmethacrylate
        Type: general
      – SubjectFull: Pixels
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      – TitleFull: Analytical figures of merit for a novel tissue imaging system.
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            NameFull: Baranov, V. I.
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              M: 05
              Text: May2017
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              Y: 2017
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