Analytical figures of merit for a novel tissue imaging system.
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| Title: | Analytical figures of merit for a novel tissue imaging system. |
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| Authors: | Straus, R. N.1, Carew, A.1, Sandkuijl, D.1, Closson, T.1, Baranov, V. I.1, Loboda, A.1 |
| Source: | JAAS (Journal of Analytical Atomic Spectrometry). May2017, Vol. 32 Issue 5, p1044-1051. 8p. |
| Subjects: | Imaging systems in biology, Laser ablation, Cytometry, Polymethylmethacrylate, Pixels |
| Abstract: | A novel laser-ablation sample introduction system has been developed for the Fluidigm® Helios™ mass cytometer to enable highly multiplexed imaging of biological samples. Herein, we report on the analytical figures of merit for this system using a slide, coated uniformly with a metal-doped PMMA film, as a test sample. The system is based on the use of a fifth harmonic Nd–YAG laser focused to a spot size of 1 micrometer or less. A fast wash-out ablation chamber provides resolution of individual plumes when operated at a 100 Hz or 200 Hz laser repetition frequency. The system exhibits a linear response to metal concentration in the sample over 4 orders of magnitude. The variation of signals generated for 1 micrometer diameter pixels is ≤15% RSD. Pixel-to-pixel sample contamination due to redeposition of the ablated material is approximately 0.6%. The variation of the ablated crater diameter with laser energy is also explored, demonstrating a linear behaviour over a limited range of laser energies. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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