Fast, sensitive, and selective gas chromatography tandem mass spectrometry method for the target analysis of chemical secretions from femoral glands in lizards.
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| Title: | Fast, sensitive, and selective gas chromatography tandem mass spectrometry method for the target analysis of chemical secretions from femoral glands in lizards. |
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| Authors: | Sáiz, Jorge1 jorge.saizg@gmail.com, García-Roa, Roberto2, Martín, José2, Gómara, Belén1 |
| Source: | Journal of Chromatography A. Sep2017, Vol. 1514, p110-119. 10p. |
| Subjects: | Gas chromatography/Mass spectrometry (GC-MS), Lizards, Sexual selection, Habitats, Secretion |
| Abstract: | Chemical signaling is a widespread mode of communication among living organisms that is used to establish social organization, territoriality and/or for mate choice. In lizards, femoral and precloacal glands are important sources of chemical signals. These glands protrude chemical secretions used to mark territories and also, to provide valuable information from the bearer to other individuals. Ecologists have studied these chemical secretions for decades in order to increase the knowledge of chemical communication in lizards. Although several studies have focused on the chemical analysis of these secretions, there is a lack of faster, more sensitive and more selective analytical methodologies for their study. In this work a new GC coupled to tandem triple quadrupole MS (GC-QqQ (MS/MS)) methodology is developed and proposed for the target study of 12 relevant compounds often found in lizard secretions ( i.e. 1-hexadecanol, palmitic acid, 1-octadecanol, oleic acid, stearic acid, 1-tetracosanol, squalene, cholesta-3,5-diene, α-tocopherol, cholesterol, ergosterol and campesterol). The method baseline-separated the analytes in less than 7 min, with instrumental limits of detection ranging from 0.04 to 6.0 ng/mL. It was possible to identify differences in the composition of the samples from the lizards analyzed, which depended on the species, the habitat occupied and the diet of the individuals. Moreover, α-tocopherol has been determined for the first time in a lizard species, which was thought to lack its expression in chemical secretions. Globally, the methodology has been proven to be a valuable alternative to other published methods with important improvements in terms of analysis time, sensitivity, and selectivity. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 124612024 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Fast, sensitive, and selective gas chromatography tandem mass spectrometry method for the target analysis of chemical secretions from femoral glands in lizards. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sáiz%2C+Jorge%22">Sáiz, Jorge</searchLink><relatesTo>1</relatesTo><i> jorge.saizg@gmail.com</i><br /><searchLink fieldCode="AR" term="%22García-Roa%2C+Roberto%22">García-Roa, Roberto</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Martín%2C+José%22">Martín, José</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Gómara%2C+Belén%22">Gómara, Belén</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Sep2017, Vol. 1514, p110-119. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Gas+chromatography%2FMass+spectrometry+%28GC-MS%29%22">Gas chromatography/Mass spectrometry (GC-MS)</searchLink><br /><searchLink fieldCode="DE" term="%22Lizards%22">Lizards</searchLink><br /><searchLink fieldCode="DE" term="%22Sexual+selection%22">Sexual selection</searchLink><br /><searchLink fieldCode="DE" term="%22Habitats%22">Habitats</searchLink><br /><searchLink fieldCode="DE" term="%22Secretion%22">Secretion</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Chemical signaling is a widespread mode of communication among living organisms that is used to establish social organization, territoriality and/or for mate choice. In lizards, femoral and precloacal glands are important sources of chemical signals. These glands protrude chemical secretions used to mark territories and also, to provide valuable information from the bearer to other individuals. Ecologists have studied these chemical secretions for decades in order to increase the knowledge of chemical communication in lizards. Although several studies have focused on the chemical analysis of these secretions, there is a lack of faster, more sensitive and more selective analytical methodologies for their study. In this work a new GC coupled to tandem triple quadrupole MS (GC-QqQ (MS/MS)) methodology is developed and proposed for the target study of 12 relevant compounds often found in lizard secretions ( i.e. 1-hexadecanol, palmitic acid, 1-octadecanol, oleic acid, stearic acid, 1-tetracosanol, squalene, cholesta-3,5-diene, α-tocopherol, cholesterol, ergosterol and campesterol). The method baseline-separated the analytes in less than 7 min, with instrumental limits of detection ranging from 0.04 to 6.0 ng/mL. It was possible to identify differences in the composition of the samples from the lizards analyzed, which depended on the species, the habitat occupied and the diet of the individuals. Moreover, α-tocopherol has been determined for the first time in a lizard species, which was thought to lack its expression in chemical secretions. Globally, the methodology has been proven to be a valuable alternative to other published methods with important improvements in terms of analysis time, sensitivity, and selectivity. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chroma.2017.07.068 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 110 Subjects: – SubjectFull: Gas chromatography/Mass spectrometry (GC-MS) Type: general – SubjectFull: Lizards Type: general – SubjectFull: Sexual selection Type: general – SubjectFull: Habitats Type: general – SubjectFull: Secretion Type: general Titles: – TitleFull: Fast, sensitive, and selective gas chromatography tandem mass spectrometry method for the target analysis of chemical secretions from femoral glands in lizards. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sáiz, Jorge – PersonEntity: Name: NameFull: García-Roa, Roberto – PersonEntity: Name: NameFull: Martín, José – PersonEntity: Name: NameFull: Gómara, Belén IsPartOfRelationships: – BibEntity: Dates: – D: 08 M: 09 Text: Sep2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 1514 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
