Percutaneous immunization with 40-nm antigen-encapsulated elastic liposomes.
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| Title: | Percutaneous immunization with 40-nm antigen-encapsulated elastic liposomes. |
|---|---|
| Authors: | Kakami, Yuki1, Takeuchi, Issei1,2, Makino, Kimiko1,2 makino@rs.noda.tus.ac.jp |
| Source: | Colloids & Surfaces A: Physicochemical & Engineering Aspects. Apr2019, Vol. 566, p128-133. 6p. |
| Subjects: | Liposomes, Immunization, Transdermal medication, Immunoglobulins, Albumins |
| Abstract: | Graphical abstract Abstract In this study, we aimed to verify the influence of the particle diameter of elastic liposomes for transdermal delivery on immune responsiveness by evaluating the amount of antibody. Albumin from chicken egg white (OVA) solution and elastic liposomes with mean volume diameter of 40 nm and 130 nm were percutaneously administrated to mice. We prepared 40-nm elastic liposomes using mechanochemical process and 130-nm elastic liposomes using extrusion process. Subcutaneous injection of OVA was also as a positive control. Forty-nanometer elastic liposomes significantly promoted antibody production compared with the OVA solution and 130-nm elastic liposomes. Furthermore, this result was nearly equivalent to that of the subcutaneous injection of OVA solution. Besides, skin permeability study shows that coumarin-6 used as a fluorescent label encapsulated in 40-nm elastic liposomes were 2.2 times more permeated than 130-nm elastic liposomes in the skin. From the observation of confocal laser microscopy images, 40-nm elastic liposomes accumulated more in the stratum corneum and deeper follicle compared to 130-nm elastic liposomes. It has been reported that particles with a diameter of 40 nm are more captured by antigen presenting cells. Thus, we considered that the 40-nm elastic liposomes are useful for percutaneous immunization. [ABSTRACT FROM AUTHOR] |
| Copyright of Colloids & Surfaces A: Physicochemical & Engineering Aspects is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 134530889 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Percutaneous immunization with 40-nm antigen-encapsulated elastic liposomes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kakami%2C+Yuki%22">Kakami, Yuki</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Takeuchi%2C+Issei%22">Takeuchi, Issei</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Makino%2C+Kimiko%22">Makino, Kimiko</searchLink><relatesTo>1,2</relatesTo><i> makino@rs.noda.tus.ac.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Colloids+%26+Surfaces+A%3A+Physicochemical+%26+Engineering+Aspects%22">Colloids & Surfaces A: Physicochemical & Engineering Aspects</searchLink>. Apr2019, Vol. 566, p128-133. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Liposomes%22">Liposomes</searchLink><br /><searchLink fieldCode="DE" term="%22Immunization%22">Immunization</searchLink><br /><searchLink fieldCode="DE" term="%22Transdermal+medication%22">Transdermal medication</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoglobulins%22">Immunoglobulins</searchLink><br /><searchLink fieldCode="DE" term="%22Albumins%22">Albumins</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Graphical abstract Abstract In this study, we aimed to verify the influence of the particle diameter of elastic liposomes for transdermal delivery on immune responsiveness by evaluating the amount of antibody. Albumin from chicken egg white (OVA) solution and elastic liposomes with mean volume diameter of 40 nm and 130 nm were percutaneously administrated to mice. We prepared 40-nm elastic liposomes using mechanochemical process and 130-nm elastic liposomes using extrusion process. Subcutaneous injection of OVA was also as a positive control. Forty-nanometer elastic liposomes significantly promoted antibody production compared with the OVA solution and 130-nm elastic liposomes. Furthermore, this result was nearly equivalent to that of the subcutaneous injection of OVA solution. Besides, skin permeability study shows that coumarin-6 used as a fluorescent label encapsulated in 40-nm elastic liposomes were 2.2 times more permeated than 130-nm elastic liposomes in the skin. From the observation of confocal laser microscopy images, 40-nm elastic liposomes accumulated more in the stratum corneum and deeper follicle compared to 130-nm elastic liposomes. It has been reported that particles with a diameter of 40 nm are more captured by antigen presenting cells. Thus, we considered that the 40-nm elastic liposomes are useful for percutaneous immunization. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Colloids & Surfaces A: Physicochemical & Engineering Aspects is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.colsurfa.2019.01.023 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 128 Subjects: – SubjectFull: Liposomes Type: general – SubjectFull: Immunization Type: general – SubjectFull: Transdermal medication Type: general – SubjectFull: Immunoglobulins Type: general – SubjectFull: Albumins Type: general Titles: – TitleFull: Percutaneous immunization with 40-nm antigen-encapsulated elastic liposomes. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kakami, Yuki – PersonEntity: Name: NameFull: Takeuchi, Issei – PersonEntity: Name: NameFull: Makino, Kimiko IsPartOfRelationships: – BibEntity: Dates: – D: 05 M: 04 Text: Apr2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 09277757 Numbering: – Type: volume Value: 566 Titles: – TitleFull: Colloids & Surfaces A: Physicochemical & Engineering Aspects Type: main |
| ResultId | 1 |
