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A microfluidic platform for the simultaneous quantification of methanogen populations in anaerobic digestion processes.

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Title: A microfluidic platform for the simultaneous quantification of methanogen populations in anaerobic digestion processes.
Authors: Mathai, Prince P.1 (AUTHOR), Dunn, Hannah M.1 (AUTHOR), Venkiteshwaran, Kaushik2 (AUTHOR), Zitomer, Daniel H.2 (AUTHOR), Maki, James S.3 (AUTHOR), Ishii, Satoshi1,4 (AUTHOR), Sadowsky, Michael J.1,4,5 (AUTHOR) sadowsky@umn.edu
Source: Environmental Microbiology. May2019, Vol. 21 Issue 5, p1798-1808. 11p.
Subjects: Anaerobic digestion, Carbon cycle, Waste treatment, Biogas production, Sewage sludge digestion, Population
Abstract: Summary: Methanogens are a diverse group of archaea that play a critical role in the global carbon cycle. The lack of appropriate molecular tools to simultaneously quantify numerous methanogenic taxa, however, has largely limited our ability to study these communities in a wide variety of habitats, such as anaerobic digesters (ADs). In this study, 34 probe‐based quantitative PCR (qPCR) assays were designed to target all known methanogenic genera within the archaeal phylum Euryarchaeota. These qPCR assays were adapted to a high‐throughput microfluidic platform, which allowed for the simultaneous detection and absolute quantification of numerous taxa in a single run. The resulting microfluidic qPCR (MFQPCR) platform was successfully used to decipher structure–function relationships among methanogenic communities in four laboratory‐scale digesters exposed to a transient organic overload. Twelve of the 34 genera targeted in the MFQPCR were detected in the ADs, similar to results obtained using high‐throughput sequencing. The MFQPCR platform and conventional qPCR assays also generated similar quantitative results. The MFQPCR tool developed here will help optimize AD technologies for efficient waste treatment and enhanced biogas production and can facilitate studies that will increase our understanding of methanogenic communities in other environments. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: A microfluidic platform for the simultaneous quantification of methanogen populations in anaerobic digestion processes.
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  Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. May2019, Vol. 21 Issue 5, p1798-1808. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Anaerobic+digestion%22">Anaerobic digestion</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+cycle%22">Carbon cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Waste+treatment%22">Waste treatment</searchLink><br /><searchLink fieldCode="DE" term="%22Biogas+production%22">Biogas production</searchLink><br /><searchLink fieldCode="DE" term="%22Sewage+sludge+digestion%22">Sewage sludge digestion</searchLink><br /><searchLink fieldCode="DE" term="%22Population%22">Population</searchLink>
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  Data: Summary: Methanogens are a diverse group of archaea that play a critical role in the global carbon cycle. The lack of appropriate molecular tools to simultaneously quantify numerous methanogenic taxa, however, has largely limited our ability to study these communities in a wide variety of habitats, such as anaerobic digesters (ADs). In this study, 34 probe‐based quantitative PCR (qPCR) assays were designed to target all known methanogenic genera within the archaeal phylum Euryarchaeota. These qPCR assays were adapted to a high‐throughput microfluidic platform, which allowed for the simultaneous detection and absolute quantification of numerous taxa in a single run. The resulting microfluidic qPCR (MFQPCR) platform was successfully used to decipher structure–function relationships among methanogenic communities in four laboratory‐scale digesters exposed to a transient organic overload. Twelve of the 34 genera targeted in the MFQPCR were detected in the ADs, similar to results obtained using high‐throughput sequencing. The MFQPCR platform and conventional qPCR assays also generated similar quantitative results. The MFQPCR tool developed here will help optimize AD technologies for efficient waste treatment and enhanced biogas production and can facilitate studies that will increase our understanding of methanogenic communities in other environments. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/1462-2920.14589
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        Type: general
      – SubjectFull: Carbon cycle
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      – SubjectFull: Waste treatment
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      – SubjectFull: Sewage sludge digestion
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      – SubjectFull: Population
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              Text: May2019
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