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Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage.

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Title: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage.
Authors: Valdiglesias, Vanessa1,2 (AUTHOR) natalia.fernandezb@udc.es, Fernández-Bertólez, Natalia2,3 (AUTHOR) Raquel.rodriguez4@udc.es, Lema-Arranz, Carlota1,2 (AUTHOR) eduardo.pasaro@udc.es, Rodríguez-Fernández, Raquel2,3 (AUTHOR) blaffon@udc.es, Pásaro, Eduardo2,3 (AUTHOR), Reis, Ana Teresa4,5,6 (AUTHOR) anateresareis@gmail.com, Teixeira, João Paulo4,5,6 (AUTHOR) jpft12@gmail.com, Costa, Carla4,5,6 (AUTHOR) carla.trindade@insa.min-saude.pt, Laffon, Blanca2,3 (AUTHOR)
Source: Nanomaterials (2079-4991). Aug2021, Vol. 11 Issue 8, p1930. 1p.
Subjects: Leukocytes, DNA damage, Metallic oxides, Metal nanoparticles, Zinc oxide, Public health
Abstract: Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO2), zinc oxide (ZnO), and cerium dioxide (CeO2) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage.
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  Data: <searchLink fieldCode="AR" term="%22Valdiglesias%2C+Vanessa%22">Valdiglesias, Vanessa</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> natalia.fernandezb@udc.es</i><br /><searchLink fieldCode="AR" term="%22Fernández-Bertólez%2C+Natalia%22">Fernández-Bertólez, Natalia</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> Raquel.rodriguez4@udc.es</i><br /><searchLink fieldCode="AR" term="%22Lema-Arranz%2C+Carlota%22">Lema-Arranz, Carlota</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> eduardo.pasaro@udc.es</i><br /><searchLink fieldCode="AR" term="%22Rodríguez-Fernández%2C+Raquel%22">Rodríguez-Fernández, Raquel</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> blaffon@udc.es</i><br /><searchLink fieldCode="AR" term="%22Pásaro%2C+Eduardo%22">Pásaro, Eduardo</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reis%2C+Ana+Teresa%22">Reis, Ana Teresa</searchLink><relatesTo>4,5,6</relatesTo> (AUTHOR)<i> anateresareis@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Teixeira%2C+João+Paulo%22">Teixeira, João Paulo</searchLink><relatesTo>4,5,6</relatesTo> (AUTHOR)<i> jpft12@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Costa%2C+Carla%22">Costa, Carla</searchLink><relatesTo>4,5,6</relatesTo> (AUTHOR)<i> carla.trindade@insa.min-saude.pt</i><br /><searchLink fieldCode="AR" term="%22Laffon%2C+Blanca%22">Laffon, Blanca</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Aug2021, Vol. 11 Issue 8, p1930. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Leukocytes%22">Leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Metallic+oxides%22">Metallic oxides</searchLink><br /><searchLink fieldCode="DE" term="%22Metal+nanoparticles%22">Metal nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Zinc+oxide%22">Zinc oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Public+health%22">Public health</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO2), zinc oxide (ZnO), and cerium dioxide (CeO2) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano11081930
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: 1930
    Subjects:
      – SubjectFull: Leukocytes
        Type: general
      – SubjectFull: DNA damage
        Type: general
      – SubjectFull: Metallic oxides
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      – SubjectFull: Metal nanoparticles
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      – SubjectFull: Zinc oxide
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      – SubjectFull: Public health
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      – TitleFull: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage.
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              Text: Aug2021
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