Lifestyle of sponge symbiont phages by host prediction and correlative microscopy.
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| Title: | Lifestyle of sponge symbiont phages by host prediction and correlative microscopy. |
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| Authors: | Jahn, M. T.1,2 (AUTHOR) martin.jahn@zoo.ox.ac.uk, Lachnit, T.3 (AUTHOR), Markert, S. M.4 (AUTHOR), Stigloher, C.4 (AUTHOR), Pita, L.1 (AUTHOR), Ribes, M.5 (AUTHOR), Dutilh, B. E.6 (AUTHOR), Hentschel, U.1,3 (AUTHOR) |
| Source: | ISME Journal: Multidisciplinary Journal of Microbial Ecology. Jul2021, Vol. 15 Issue 7, p2001-2011. 11p. |
| Abstract: | Bacteriophages (phages) are ubiquitous elements in nature, but their ecology and role in animals remains little understood. Sponges represent the oldest known extant animal-microbe symbiosis and are associated with dense and diverse microbial consortia. Here we investigate the tripartite interaction between phages, bacterial symbionts, and the sponge host. We combined imaging and bioinformatics to tackle important questions on who the phage hosts are and what the replication mode and spatial distribution within the animal is. This approach led to the discovery of distinct phage-microbe infection networks in sponge versus seawater microbiomes. A new correlative in situ imaging approach ('PhageFISH-CLEM') localised phages within bacterial symbiont cells, but also within phagocytotically active sponge cells. We postulate that the phagocytosis of free virions by sponge cells modulates phage-bacteria ratios and ultimately controls infection dynamics. Prediction of phage replication strategies indicated a distinct pattern, where lysogeny dominates the sponge microbiome, likely fostered by sponge host-mediated virion clearance, while lysis dominates in seawater. Collectively, this work provides new insights into phage ecology within sponges, highlighting the importance of tripartite animal-phage-bacterium interplay in holobiont functioning. We anticipate that our imaging approach will be instrumental to further understanding of viral distribution and cellular association in animal hosts. [ABSTRACT FROM AUTHOR] |
| Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 156398311 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Lifestyle of sponge symbiont phages by host prediction and correlative microscopy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jahn%2C+M%2E+T%2E%22">Jahn, M. T.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> martin.jahn@zoo.ox.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Lachnit%2C+T%2E%22">Lachnit, T.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Markert%2C+S%2E+M%2E%22">Markert, S. M.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stigloher%2C+C%2E%22">Stigloher, C.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pita%2C+L%2E%22">Pita, L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ribes%2C+M%2E%22">Ribes, M.</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dutilh%2C+B%2E+E%2E%22">Dutilh, B. E.</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hentschel%2C+U%2E%22">Hentschel, U.</searchLink><relatesTo>1,3</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22ISME+Journal%3A+Multidisciplinary+Journal+of+Microbial+Ecology%22">ISME Journal: Multidisciplinary Journal of Microbial Ecology</searchLink>. Jul2021, Vol. 15 Issue 7, p2001-2011. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Bacteriophages (phages) are ubiquitous elements in nature, but their ecology and role in animals remains little understood. Sponges represent the oldest known extant animal-microbe symbiosis and are associated with dense and diverse microbial consortia. Here we investigate the tripartite interaction between phages, bacterial symbionts, and the sponge host. We combined imaging and bioinformatics to tackle important questions on who the phage hosts are and what the replication mode and spatial distribution within the animal is. This approach led to the discovery of distinct phage-microbe infection networks in sponge versus seawater microbiomes. A new correlative in situ imaging approach ('PhageFISH-CLEM') localised phages within bacterial symbiont cells, but also within phagocytotically active sponge cells. We postulate that the phagocytosis of free virions by sponge cells modulates phage-bacteria ratios and ultimately controls infection dynamics. Prediction of phage replication strategies indicated a distinct pattern, where lysogeny dominates the sponge microbiome, likely fostered by sponge host-mediated virion clearance, while lysis dominates in seawater. Collectively, this work provides new insights into phage ecology within sponges, highlighting the importance of tripartite animal-phage-bacterium interplay in holobiont functioning. We anticipate that our imaging approach will be instrumental to further understanding of viral distribution and cellular association in animal hosts. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/s41396-021-00900-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 2001 Titles: – TitleFull: Lifestyle of sponge symbiont phages by host prediction and correlative microscopy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jahn, M. T. – PersonEntity: Name: NameFull: Lachnit, T. – PersonEntity: Name: NameFull: Markert, S. M. – PersonEntity: Name: NameFull: Stigloher, C. – PersonEntity: Name: NameFull: Pita, L. – PersonEntity: Name: NameFull: Ribes, M. – PersonEntity: Name: NameFull: Dutilh, B. E. – PersonEntity: Name: NameFull: Hentschel, U. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 17517362 Numbering: – Type: volume Value: 15 – Type: issue Value: 7 Titles: – TitleFull: ISME Journal: Multidisciplinary Journal of Microbial Ecology Type: main |
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