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Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.

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Title: Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.
Authors: Haas, Julian1,2, Hayward, Angela3, Buer, Benjamin2, Maiwald, Frank2, Nebelsiek, Birgit2, Glaubitz, Johannes2, Bass, Chris3 c.bass@exeter.ac.uk, Nauen, Ralf2 ralf.nauen@bayer.com
Source: Proceedings of the National Academy of Sciences of the United States of America. 6/28/2022, Vol. 119 Issue 26, p1-10. 25p.
Subjects: Pollinators, Insecticides, Honeybees, Bees, Numbers of species
Abstract: The regulatory process for assessing the risks of pesticides to bees relies heavily on the use of the honeybee, Apis mellifera, as a model for other bee species. However, the validity of using A. mellifera as a surrogate for other Apis and non-Apis bees in pesticide risk assessment has been questioned. Related to this line of research, recent work on A. mellifera has shown that specific P450 enzymes belonging to the CYP9Q subfamily act as critically important determinants of insecticide sensitivity in this species by efficiently detoxifying certain insecticide chemotypes. However, the extent to which the presence of functional orthologs of these enzymes is conserved across the diversity of bees is unclear. Here we used a phylogenomic approach to identify > 100 putative CYP9Q functional orthologs across 75 bee species encompassing all major bee families. Functional analysis of 26 P450s from 20 representative bee species revealed that P450- mediated detoxification of certain systemic insecticides, including the neonicotinoid thiacloprid and the butenolide flupyradifurone, is conserved across all major bee pollinator families. However, our analyses also reveal that CYP9Q-related genes are not universal to all bee species, with some Megachilidae species lacking such genes. Thus, our results reveal an evolutionary conserved capacity to metabolize certain insecticides across all major bee families while identifying a small number of bee species where this function may have been lost. Furthermore, they illustrate the potential of a toxicogenomic approach to inform pesticide risk assessment for nonmanaged bee species by predicting the capability of bee pollinator species to break down synthetic insecticides. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.
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  Data: <searchLink fieldCode="AR" term="%22Haas%2C+Julian%22">Haas, Julian</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Hayward%2C+Angela%22">Hayward, Angela</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Buer%2C+Benjamin%22">Buer, Benjamin</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Maiwald%2C+Frank%22">Maiwald, Frank</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Nebelsiek%2C+Birgit%22">Nebelsiek, Birgit</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Glaubitz%2C+Johannes%22">Glaubitz, Johannes</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bass%2C+Chris%22">Bass, Chris</searchLink><relatesTo>3</relatesTo><i> c.bass@exeter.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Nauen%2C+Ralf%22">Nauen, Ralf</searchLink><relatesTo>2</relatesTo><i> ralf.nauen@bayer.com</i>
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  Data: <searchLink fieldCode="DE" term="%22Pollinators%22">Pollinators</searchLink><br /><searchLink fieldCode="DE" term="%22Insecticides%22">Insecticides</searchLink><br /><searchLink fieldCode="DE" term="%22Honeybees%22">Honeybees</searchLink><br /><searchLink fieldCode="DE" term="%22Bees%22">Bees</searchLink><br /><searchLink fieldCode="DE" term="%22Numbers+of+species%22">Numbers of species</searchLink>
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  Data: The regulatory process for assessing the risks of pesticides to bees relies heavily on the use of the honeybee, Apis mellifera, as a model for other bee species. However, the validity of using A. mellifera as a surrogate for other Apis and non-Apis bees in pesticide risk assessment has been questioned. Related to this line of research, recent work on A. mellifera has shown that specific P450 enzymes belonging to the CYP9Q subfamily act as critically important determinants of insecticide sensitivity in this species by efficiently detoxifying certain insecticide chemotypes. However, the extent to which the presence of functional orthologs of these enzymes is conserved across the diversity of bees is unclear. Here we used a phylogenomic approach to identify > 100 putative CYP9Q functional orthologs across 75 bee species encompassing all major bee families. Functional analysis of 26 P450s from 20 representative bee species revealed that P450- mediated detoxification of certain systemic insecticides, including the neonicotinoid thiacloprid and the butenolide flupyradifurone, is conserved across all major bee pollinator families. However, our analyses also reveal that CYP9Q-related genes are not universal to all bee species, with some Megachilidae species lacking such genes. Thus, our results reveal an evolutionary conserved capacity to metabolize certain insecticides across all major bee families while identifying a small number of bee species where this function may have been lost. Furthermore, they illustrate the potential of a toxicogenomic approach to inform pesticide risk assessment for nonmanaged bee species by predicting the capability of bee pollinator species to break down synthetic insecticides. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1073/pnas.2205850119
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        Text: English
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      – SubjectFull: Pollinators
        Type: general
      – SubjectFull: Insecticides
        Type: general
      – SubjectFull: Honeybees
        Type: general
      – SubjectFull: Bees
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      – SubjectFull: Numbers of species
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      – TitleFull: Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.
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            NameFull: Haas, Julian
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              M: 06
              Text: 6/28/2022
              Type: published
              Y: 2022
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