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Effect of dental composite dust on human gingival keratinocytes.

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Title: Effect of dental composite dust on human gingival keratinocytes.
Authors: Himmelsbach, Sabrina1,2 (AUTHOR), Steinberg, Thorsten2 (AUTHOR), Tomakidi, Pascal2 (AUTHOR), Garcia-Käufer, Manuel3 (AUTHOR), Hellwig, Elmar1 (AUTHOR), Polydorou, Olga1 (AUTHOR) olga.polydorou@uniklinik-freiburg.de
Source: Dental Materials. Nov2023, Vol. 39 Issue 11, p994-1003. 10p.
Subjects: Dental materials, Extracellular matrix proteins, Dust, Particle size distribution, Keratinocytes
Abstract: The aim was to investigate the effect of particles released during grinding of dental composites on human gingival keratinocytes (HGK). Specimens from Filtek™ Supreme XTE and ceram.x® universal were prepared and ground to dust. The dust was filtered (≤ 5 µm) and the particle size distribution was examined using NANO-flex®-180° dynamic light scattering (DLS). Suspensions at five concentrations (3, 10, 30, 100 and 300 µg/mL) were prepared using keratinocyte growth medium (KGM). These suspensions, as well as a positive (CuO) and a negative control (KGM) were added to HGK. The cells treated with Filtek™ Supreme XTE suspensions were analyzed by real-time monitoring using RTCA iCELLigence™. In addition, light and scanning electron microscopic images of the exposed cells were taken. Indirect immunofluorescence staining was performed to detect the extracellular matrix protein fibronectin. In distilled water, DLS showed similar particles' range (171.9 nm- 2.7 µm) for both composites. In saliva, larger particles were detected (Filtek™ Supreme XTE: 243 nm-6,5 µm; ceram.x® universal: 204 nm- 4,6 µm). iCELLigence™ revealed similar results of cell growth parameters for HGK incubated with composite dust (≤ 5 µm) at different concentrations. The microscopic images indicated unaltered cell structures and formation of large agglomerates with high particle concentration (> 100 µg/mL). Exposure to composite dust resulted in upregulation of fibronectin expression. Grinding of dental composite materials generates dust particles of different sizes. The particle size distribution seems to be more influenced by the suspending medium than the material itself. While cell growth of HGK seem not to be affected by the particles, an upregulation of fibronectin in the intercellular space concomitant by increasing particle concentration may indicate an increase of cell migration/mobility. • Grinding of composites can generate dust particles in the nanoscale range. • The surrounding medium influences the size of composite dust particles. • HGK exposure to composite dust results in upregulation of fibronectin in the intercellular space. [ABSTRACT FROM AUTHOR]
Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: Effect of dental composite dust on human gingival keratinocytes.
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  Data: <searchLink fieldCode="AR" term="%22Himmelsbach%2C+Sabrina%22">Himmelsbach, Sabrina</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Steinberg%2C+Thorsten%22">Steinberg, Thorsten</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tomakidi%2C+Pascal%22">Tomakidi, Pascal</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Garcia-Käufer%2C+Manuel%22">Garcia-Käufer, Manuel</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hellwig%2C+Elmar%22">Hellwig, Elmar</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Polydorou%2C+Olga%22">Polydorou, Olga</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> olga.polydorou@uniklinik-freiburg.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Dental+Materials%22">Dental Materials</searchLink>. Nov2023, Vol. 39 Issue 11, p994-1003. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Dental+materials%22">Dental materials</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+matrix+proteins%22">Extracellular matrix proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Dust%22">Dust</searchLink><br /><searchLink fieldCode="DE" term="%22Particle+size+distribution%22">Particle size distribution</searchLink><br /><searchLink fieldCode="DE" term="%22Keratinocytes%22">Keratinocytes</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: The aim was to investigate the effect of particles released during grinding of dental composites on human gingival keratinocytes (HGK). Specimens from Filtek™ Supreme XTE and ceram.x® universal were prepared and ground to dust. The dust was filtered (≤ 5 µm) and the particle size distribution was examined using NANO-flex®-180° dynamic light scattering (DLS). Suspensions at five concentrations (3, 10, 30, 100 and 300 µg/mL) were prepared using keratinocyte growth medium (KGM). These suspensions, as well as a positive (CuO) and a negative control (KGM) were added to HGK. The cells treated with Filtek™ Supreme XTE suspensions were analyzed by real-time monitoring using RTCA iCELLigence™. In addition, light and scanning electron microscopic images of the exposed cells were taken. Indirect immunofluorescence staining was performed to detect the extracellular matrix protein fibronectin. In distilled water, DLS showed similar particles' range (171.9 nm- 2.7 µm) for both composites. In saliva, larger particles were detected (Filtek™ Supreme XTE: 243 nm-6,5 µm; ceram.x® universal: 204 nm- 4,6 µm). iCELLigence™ revealed similar results of cell growth parameters for HGK incubated with composite dust (≤ 5 µm) at different concentrations. The microscopic images indicated unaltered cell structures and formation of large agglomerates with high particle concentration (> 100 µg/mL). Exposure to composite dust resulted in upregulation of fibronectin expression. Grinding of dental composite materials generates dust particles of different sizes. The particle size distribution seems to be more influenced by the suspending medium than the material itself. While cell growth of HGK seem not to be affected by the particles, an upregulation of fibronectin in the intercellular space concomitant by increasing particle concentration may indicate an increase of cell migration/mobility. • Grinding of composites can generate dust particles in the nanoscale range. • The surrounding medium influences the size of composite dust particles. • HGK exposure to composite dust results in upregulation of fibronectin in the intercellular space. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.dental.2023.09.004
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 994
    Subjects:
      – SubjectFull: Dental materials
        Type: general
      – SubjectFull: Extracellular matrix proteins
        Type: general
      – SubjectFull: Dust
        Type: general
      – SubjectFull: Particle size distribution
        Type: general
      – SubjectFull: Keratinocytes
        Type: general
    Titles:
      – TitleFull: Effect of dental composite dust on human gingival keratinocytes.
        Type: main
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            NameFull: Himmelsbach, Sabrina
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            NameFull: Steinberg, Thorsten
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            NameFull: Garcia-Käufer, Manuel
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            NameFull: Hellwig, Elmar
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            – D: 01
              M: 11
              Text: Nov2023
              Type: published
              Y: 2023
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              Value: 39
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