Effects of Cationic and Anionic Surfaces on the Perpendicular and Lateral Forces and Binding of Aspergillus niger Conidia.
Saved in:
| Title: | Effects of Cationic and Anionic Surfaces on the Perpendicular and Lateral Forces and Binding of Aspergillus niger Conidia. |
|---|---|
| Authors: | Whitehead, Kathryn A.1 (AUTHOR) k.a.whitehead@mmu.ac.uk, Lynch, Stephen2 (AUTHOR) s.lynch@mmu.ac.uk, Amin, Mohsin1 (AUTHOR) chris.liauw.curvi.hifi@googlemail.com, Deisenroth, Ted3 (AUTHOR) ted.deisenroth@basf.com, Liauw, Christopher M.1 (AUTHOR), Verran, Joanna1 (AUTHOR) |
| Source: | Nanomaterials (2079-4991). Nov2023, Vol. 13 Issue 22, p2932. 15p. |
| Subjects: | Aspergillus niger, Conidia, Binding site assay, Surface topography |
| Abstract: | The binding of conidia to surfaces is a prerequisite for biofouling by fungal species. In this study, Aspergillus niger subtypes 1957 and 1988 were used which produced differently shaped conidia (round or spikey respectively). Test surfaces were characterised for their surface topography, wettability, and hardness. Conidial assays included perpendicular and lateral force measurements, as well as attachment, adhesion and retention assays. Anionic surfaces were less rough (Ra 2.4 nm), less wettable (54°) and harder (0.72 GPa) than cationic surfaces (Ra 5.4 nm, 36° and 0.5 GPa, respectively). Perpendicular and lateral force assays demonstrated that both types of conidia adhered with more force to the anionic surfaces and were influenced by surface wettability. Following the binding assays, fewer A. niger 1957 and A. niger 1988 conidia bound to the anionic surface. However, surface wettability affected the density and dispersion of the conidia on the coatings, whilst clustering was affected by their spore shapes. This work demonstrated that anionic surfaces were more repulsive to A. niger 1998 spores than cationic surfaces were, but once attached, the conidia bound more firmly to the anionic surfaces. This work informs on the importance of understanding how conidia become tightly bound to surfaces, which can be used to prevent biofouling. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 173868333 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Effects of Cationic and Anionic Surfaces on the Perpendicular and Lateral Forces and Binding of Aspergillus niger Conidia. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Whitehead%2C+Kathryn+A%2E%22">Whitehead, Kathryn A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> k.a.whitehead@mmu.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Lynch%2C+Stephen%22">Lynch, Stephen</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> s.lynch@mmu.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Amin%2C+Mohsin%22">Amin, Mohsin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> chris.liauw.curvi.hifi@googlemail.com</i><br /><searchLink fieldCode="AR" term="%22Deisenroth%2C+Ted%22">Deisenroth, Ted</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> ted.deisenroth@basf.com</i><br /><searchLink fieldCode="AR" term="%22Liauw%2C+Christopher+M%2E%22">Liauw, Christopher M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Verran%2C+Joanna%22">Verran, Joanna</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Nov2023, Vol. 13 Issue 22, p2932. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Aspergillus+niger%22">Aspergillus niger</searchLink><br /><searchLink fieldCode="DE" term="%22Conidia%22">Conidia</searchLink><br /><searchLink fieldCode="DE" term="%22Binding+site+assay%22">Binding site assay</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+topography%22">Surface topography</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The binding of conidia to surfaces is a prerequisite for biofouling by fungal species. In this study, Aspergillus niger subtypes 1957 and 1988 were used which produced differently shaped conidia (round or spikey respectively). Test surfaces were characterised for their surface topography, wettability, and hardness. Conidial assays included perpendicular and lateral force measurements, as well as attachment, adhesion and retention assays. Anionic surfaces were less rough (Ra 2.4 nm), less wettable (54°) and harder (0.72 GPa) than cationic surfaces (Ra 5.4 nm, 36° and 0.5 GPa, respectively). Perpendicular and lateral force assays demonstrated that both types of conidia adhered with more force to the anionic surfaces and were influenced by surface wettability. Following the binding assays, fewer A. niger 1957 and A. niger 1988 conidia bound to the anionic surface. However, surface wettability affected the density and dispersion of the conidia on the coatings, whilst clustering was affected by their spore shapes. This work demonstrated that anionic surfaces were more repulsive to A. niger 1998 spores than cationic surfaces were, but once attached, the conidia bound more firmly to the anionic surfaces. This work informs on the importance of understanding how conidia become tightly bound to surfaces, which can be used to prevent biofouling. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=173868333 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano13222932 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 2932 Subjects: – SubjectFull: Aspergillus niger Type: general – SubjectFull: Conidia Type: general – SubjectFull: Binding site assay Type: general – SubjectFull: Surface topography Type: general Titles: – TitleFull: Effects of Cationic and Anionic Surfaces on the Perpendicular and Lateral Forces and Binding of Aspergillus niger Conidia. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Whitehead, Kathryn A. – PersonEntity: Name: NameFull: Lynch, Stephen – PersonEntity: Name: NameFull: Amin, Mohsin – PersonEntity: Name: NameFull: Deisenroth, Ted – PersonEntity: Name: NameFull: Liauw, Christopher M. – PersonEntity: Name: NameFull: Verran, Joanna IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 11 Text: Nov2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 13 – Type: issue Value: 22 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
| ResultId | 1 |
