High-resolution computation predicts that low dissolved CO concentrations and CO gradients promote ethanol production at industrial-scale gas fermentation.
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| Title: | High-resolution computation predicts that low dissolved CO concentrations and CO gradients promote ethanol production at industrial-scale gas fermentation. |
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| Authors: | Puiman, Lars1 (AUTHOR), Almeida Benalcázar, Eduardo1 (AUTHOR), Picioreanu, Cristian2 (AUTHOR), Noorman, Henk J.1,3 (AUTHOR), Haringa, Cees1 (AUTHOR) C.Haringa@tudelft.nl |
| Source: | Biochemical Engineering Journal. Jul2024, Vol. 207, pN.PAG-N.PAG. 1p. |
| Subjects: | Concentration gradient, Ethanol, Fermentation, Gas dynamics, Experimental literature, Anaerobic reactors, Ferredoxins, NAD (Coenzyme) |
| Abstract: | Gradients in dissolved gas concentrations are expected to affect the performance of large reactors for anaerobic gas (CO, H 2 , CO 2) fermentation. To study how these gradients, and the dissolved gas concentration level itself, influence the productivity of the desired product ethanol and the product spectrum of C. autoethanogenum , we coupled a CFD model of an industrial-scale gas fermentor to a metabolic kinetic model for a wide range of metabolic regimes. Our model results, together with literature experimental data and a model with constant dissolved gas concentrations, indicate high ethanol specificity at low dissolved CO concentrations, with acetate reduction to ethanol at very low dissolved CO concentrations and combined ethanol and acetate production at higher CO concentrations. The gradient was predicted to increase both the biomass-specific ethanol production rate and the electron-to-ethanol yield by ∼25%. This might be due to intensified ferredoxin and NAD+ redox cycles, with the rate of the Rnf complex – a critical enzyme for energy conservation – as key driver towards ethanol production, all at the expense of a reduced flux to acetate. We present improved mechanistic understanding of the gas fermentation process, and novel leads for optimization and fundamental research, by coupling observations from various down-scaled lab experiments to expected microbial lifelines in an industrial-scale reactor. [Display omitted] • Fully coupled CFD model with metabolic dynamics for industrial-scale gas fermentor. • Low CO concentrations relate to ethanol while high c L,CO relate to acetate production. • The dissolved CO concentration is a major determinant of the product spectrum. • Large-scale dissolved CO concentration gradient enhances ethanol production by ∼25%. • Enhanced mechanistic understanding of gas fermentation, confirmed with experiments. [ABSTRACT FROM AUTHOR] |
| Copyright of Biochemical Engineering Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 177088218 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: High-resolution computation predicts that low dissolved CO concentrations and CO gradients promote ethanol production at industrial-scale gas fermentation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Puiman%2C+Lars%22">Puiman, Lars</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Almeida+Benalcázar%2C+Eduardo%22">Almeida Benalcázar, Eduardo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Picioreanu%2C+Cristian%22">Picioreanu, Cristian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Noorman%2C+Henk+J%2E%22">Noorman, Henk J.</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Haringa%2C+Cees%22">Haringa, Cees</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> C.Haringa@tudelft.nl</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biochemical+Engineering+Journal%22">Biochemical Engineering Journal</searchLink>. Jul2024, Vol. 207, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Concentration+gradient%22">Concentration gradient</searchLink><br /><searchLink fieldCode="DE" term="%22Ethanol%22">Ethanol</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Gas+dynamics%22">Gas dynamics</searchLink><br /><searchLink fieldCode="DE" term="%22Experimental+literature%22">Experimental literature</searchLink><br /><searchLink fieldCode="DE" term="%22Anaerobic+reactors%22">Anaerobic reactors</searchLink><br /><searchLink fieldCode="DE" term="%22Ferredoxins%22">Ferredoxins</searchLink><br /><searchLink fieldCode="DE" term="%22NAD+%28Coenzyme%29%22">NAD (Coenzyme)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Gradients in dissolved gas concentrations are expected to affect the performance of large reactors for anaerobic gas (CO, H 2 , CO 2) fermentation. To study how these gradients, and the dissolved gas concentration level itself, influence the productivity of the desired product ethanol and the product spectrum of C. autoethanogenum , we coupled a CFD model of an industrial-scale gas fermentor to a metabolic kinetic model for a wide range of metabolic regimes. Our model results, together with literature experimental data and a model with constant dissolved gas concentrations, indicate high ethanol specificity at low dissolved CO concentrations, with acetate reduction to ethanol at very low dissolved CO concentrations and combined ethanol and acetate production at higher CO concentrations. The gradient was predicted to increase both the biomass-specific ethanol production rate and the electron-to-ethanol yield by ∼25%. This might be due to intensified ferredoxin and NAD+ redox cycles, with the rate of the Rnf complex – a critical enzyme for energy conservation – as key driver towards ethanol production, all at the expense of a reduced flux to acetate. We present improved mechanistic understanding of the gas fermentation process, and novel leads for optimization and fundamental research, by coupling observations from various down-scaled lab experiments to expected microbial lifelines in an industrial-scale reactor. [Display omitted] • Fully coupled CFD model with metabolic dynamics for industrial-scale gas fermentor. • Low CO concentrations relate to ethanol while high c L,CO relate to acetate production. • The dissolved CO concentration is a major determinant of the product spectrum. • Large-scale dissolved CO concentration gradient enhances ethanol production by ∼25%. • Enhanced mechanistic understanding of gas fermentation, confirmed with experiments. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biochemical Engineering Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.bej.2024.109330 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Concentration gradient Type: general – SubjectFull: Ethanol Type: general – SubjectFull: Fermentation Type: general – SubjectFull: Gas dynamics Type: general – SubjectFull: Experimental literature Type: general – SubjectFull: Anaerobic reactors Type: general – SubjectFull: Ferredoxins Type: general – SubjectFull: NAD (Coenzyme) Type: general Titles: – TitleFull: High-resolution computation predicts that low dissolved CO concentrations and CO gradients promote ethanol production at industrial-scale gas fermentation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Puiman, Lars – PersonEntity: Name: NameFull: Almeida Benalcázar, Eduardo – PersonEntity: Name: NameFull: Picioreanu, Cristian – PersonEntity: Name: NameFull: Noorman, Henk J. – PersonEntity: Name: NameFull: Haringa, Cees IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 1369703X Numbering: – Type: volume Value: 207 Titles: – TitleFull: Biochemical Engineering Journal Type: main |
| ResultId | 1 |
