Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles.
Saved in:
| Title: | Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles. |
|---|---|
| Authors: | Gunatilake, Udara Bimendra1 (AUTHOR) gautier.felix@umontpellier.fr, Pérez-López, Briza2 (AUTHOR) briza.perez@vhir.org, Urpi, Maria2 (AUTHOR) maria.urpi@vhir.org, Prat-Trunas, Judit2,3 (AUTHOR) judit.prat@vhir.org, Carrera-Cardona, Gerard2 (AUTHOR) gerard.carrera@alumni.vhir.org, Félix, Gautier1 (AUTHOR) saad.sene@umontpellier.fr, Sene, Saad1 (AUTHOR) mickael.beaudhuin@umontpellier.fr, Beaudhuin, Mickaël1 (AUTHOR) yannick.guari@umontpellier.fr, Dupin, Jean-Charles4 (AUTHOR) jean-charles.dupin@univ-pau.fr, Allouche, Joachim4 (AUTHOR) joachim.allouche@univ-pau.fr, Guari, Yannick1 (AUTHOR) joulia.larionova@umontpellier.fr, Larionova, Joulia1 (AUTHOR), Baldrich, Eva2,5 (AUTHOR) udara-bimendra-gunatilake.kekulupolage@umontpellier.fr |
| Source: | Nanomaterials (2079-4991). Jan2025, Vol. 15 Issue 1, p41. 21p. |
| Subjects: | Horseradish peroxidase, Coordination polymers, Chemical stability, Catalytic activity, Sodium azide |
| Abstract: | Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe3+/Fe2+) of Prussian blue (PB), which have the potential to function as catalytically active centres. The decoration of PBNPs with desired functional polymers (such as amino- or carboxylate-based) primarily facilitates the subsequent linkage of biomolecules to the nanoparticles for their use in biosensor applications. Thus, the elucidation of the catalytic POD mimicry of these systems is of significant scientific interest but has not been investigated in depth yet. In this report, we studied a series of poly(ethyleneimine) (PEI)-mediated PBNPs (PB/PEI NPs) prepared using various synthesis protocols. The resulting range of particles with varying size (~19–92 nm) and shape combinations were characterised in order to gain insights into their physicochemical properties. The POD-like nanozyme activity of these nanoparticles was then investigated by utilising a 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 system, with the catalytic performance of the natural enzyme horseradish peroxidase (HRP) serving as a point of comparison. It was shown that most PB/PEI NPs displayed higher catalytic activity than the PBNPs, with higher activity observed in particles of smaller size, higher Fe content, and higher Fe2+/Fe3+ ratio. Furthermore, the nanoparticles demonstrated enhanced chemical stability in the presence of acid, sodium azide, or high concentrations of H2O2 when compared to HRP, confirming the viability of PB/PEI NPs as a promising nanozymatic material. This study disseminates fundamental knowledge on PB/PEI NPs and their POD-like activities, which will facilitate the selection of an appropriate particle type for future biosensor applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 182465882 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Gunatilake%2C+Udara+Bimendra%22">Gunatilake, Udara Bimendra</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> gautier.felix@umontpellier.fr</i><br /><searchLink fieldCode="AR" term="%22Pérez-López%2C+Briza%22">Pérez-López, Briza</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> briza.perez@vhir.org</i><br /><searchLink fieldCode="AR" term="%22Urpi%2C+Maria%22">Urpi, Maria</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> maria.urpi@vhir.org</i><br /><searchLink fieldCode="AR" term="%22Prat-Trunas%2C+Judit%22">Prat-Trunas, Judit</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> judit.prat@vhir.org</i><br /><searchLink fieldCode="AR" term="%22Carrera-Cardona%2C+Gerard%22">Carrera-Cardona, Gerard</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> gerard.carrera@alumni.vhir.org</i><br /><searchLink fieldCode="AR" term="%22Félix%2C+Gautier%22">Félix, Gautier</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> saad.sene@umontpellier.fr</i><br /><searchLink fieldCode="AR" term="%22Sene%2C+Saad%22">Sene, Saad</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mickael.beaudhuin@umontpellier.fr</i><br /><searchLink fieldCode="AR" term="%22Beaudhuin%2C+Mickaël%22">Beaudhuin, Mickaël</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> yannick.guari@umontpellier.fr</i><br /><searchLink fieldCode="AR" term="%22Dupin%2C+Jean-Charles%22">Dupin, Jean-Charles</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> jean-charles.dupin@univ-pau.fr</i><br /><searchLink fieldCode="AR" term="%22Allouche%2C+Joachim%22">Allouche, Joachim</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> joachim.allouche@univ-pau.fr</i><br /><searchLink fieldCode="AR" term="%22Guari%2C+Yannick%22">Guari, Yannick</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> joulia.larionova@umontpellier.fr</i><br /><searchLink fieldCode="AR" term="%22Larionova%2C+Joulia%22">Larionova, Joulia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Baldrich%2C+Eva%22">Baldrich, Eva</searchLink><relatesTo>2,5</relatesTo> (AUTHOR)<i> udara-bimendra-gunatilake.kekulupolage@umontpellier.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Jan2025, Vol. 15 Issue 1, p41. 21p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Horseradish+peroxidase%22">Horseradish peroxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Coordination+polymers%22">Coordination polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+stability%22">Chemical stability</searchLink><br /><searchLink fieldCode="DE" term="%22Catalytic+activity%22">Catalytic activity</searchLink><br /><searchLink fieldCode="DE" term="%22Sodium+azide%22">Sodium azide</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe3+/Fe2+) of Prussian blue (PB), which have the potential to function as catalytically active centres. The decoration of PBNPs with desired functional polymers (such as amino- or carboxylate-based) primarily facilitates the subsequent linkage of biomolecules to the nanoparticles for their use in biosensor applications. Thus, the elucidation of the catalytic POD mimicry of these systems is of significant scientific interest but has not been investigated in depth yet. In this report, we studied a series of poly(ethyleneimine) (PEI)-mediated PBNPs (PB/PEI NPs) prepared using various synthesis protocols. The resulting range of particles with varying size (~19–92 nm) and shape combinations were characterised in order to gain insights into their physicochemical properties. The POD-like nanozyme activity of these nanoparticles was then investigated by utilising a 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 system, with the catalytic performance of the natural enzyme horseradish peroxidase (HRP) serving as a point of comparison. It was shown that most PB/PEI NPs displayed higher catalytic activity than the PBNPs, with higher activity observed in particles of smaller size, higher Fe content, and higher Fe2+/Fe3+ ratio. Furthermore, the nanoparticles demonstrated enhanced chemical stability in the presence of acid, sodium azide, or high concentrations of H2O2 when compared to HRP, confirming the viability of PB/PEI NPs as a promising nanozymatic material. This study disseminates fundamental knowledge on PB/PEI NPs and their POD-like activities, which will facilitate the selection of an appropriate particle type for future biosensor applications. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=182465882 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano15010041 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 21 StartPage: 41 Subjects: – SubjectFull: Horseradish peroxidase Type: general – SubjectFull: Coordination polymers Type: general – SubjectFull: Chemical stability Type: general – SubjectFull: Catalytic activity Type: general – SubjectFull: Sodium azide Type: general Titles: – TitleFull: Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Gunatilake, Udara Bimendra – PersonEntity: Name: NameFull: Pérez-López, Briza – PersonEntity: Name: NameFull: Urpi, Maria – PersonEntity: Name: NameFull: Prat-Trunas, Judit – PersonEntity: Name: NameFull: Carrera-Cardona, Gerard – PersonEntity: Name: NameFull: Félix, Gautier – PersonEntity: Name: NameFull: Sene, Saad – PersonEntity: Name: NameFull: Beaudhuin, Mickaël – PersonEntity: Name: NameFull: Dupin, Jean-Charles – PersonEntity: Name: NameFull: Allouche, Joachim – PersonEntity: Name: NameFull: Guari, Yannick – PersonEntity: Name: NameFull: Larionova, Joulia – PersonEntity: Name: NameFull: Baldrich, Eva IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 15 – Type: issue Value: 1 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
| ResultId | 1 |
