Quantitative evaluation of composition and biomolecular mapping of macrofungi spores by Raman spectroscopy.
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| Title: | Quantitative evaluation of composition and biomolecular mapping of macrofungi spores by Raman spectroscopy. |
|---|---|
| Authors: | Shvets, Petr1 (AUTHOR) pshvets@kantiana.ru, Goikhman, Aleksandr2 (AUTHOR) |
| Source: | Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Apr2025, Vol. 331, pN.PAG-N.PAG. 1p. |
| Subjects: | Fungal proteins, Fungal spores, Organic compounds, Saccharides, Amylose |
| Abstract: | [Display omitted] • Raman spectroscopy was used to reveal the chemical composition of fungal spores. • In lipid-based spores, we determined chain length and unsaturation of triacylglycerols. • In protein-based spores, we determined the content of some amino acids and DNA. • The spores contain sugars (trehalose) and sometimes polysaccharides (amylose). • For larger spores, we built maps of key component distributions (proteins and lipids). Raman spectroscopy is a widely used technique for detecting various chemical compounds in organic matter and creating high-resolution, label-free maps on the level of individual cells. One of the main advantages of this technique is its ability to study samples in vivo without special pretreatment. However, it is rarely used to determine the ratios between different substances or to conduct in-depth quantitative analysis of the obtained spectra. In our study, we establish ratiometric equations that enable the estimation of mass concentrations of triacylglycerols, proteins, sugars, polysaccharides, and DNA. We demonstrate that it is possible to determine the average unsaturation and chain length from the spectra of lipids and concentrations of phenylalanine, tyrosine, and tryptophan from the spectra of proteins. We apply the derived equations to the Raman spectra of fungal spores from over 70 different species of macrofungi, providing a comprehensive characterization of the lipids, proteins, and polysaccharides present in the spores. We believe that our study not only contributes valuable fundamental knowledge to the field of mycology but also lays the groundwork for the spectral quantification of any organic material. So, our approach may be applicable in areas such as food diagnostics. [ABSTRACT FROM AUTHOR] |
| Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 183339552 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Quantitative evaluation of composition and biomolecular mapping of macrofungi spores by Raman spectroscopy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Shvets%2C+Petr%22">Shvets, Petr</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> pshvets@kantiana.ru</i><br /><searchLink fieldCode="AR" term="%22Goikhman%2C+Aleksandr%22">Goikhman, Aleksandr</searchLink><relatesTo>2</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Apr2025, Vol. 331, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Fungal+proteins%22">Fungal proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Fungal+spores%22">Fungal spores</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+compounds%22">Organic compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharides%22">Saccharides</searchLink><br /><searchLink fieldCode="DE" term="%22Amylose%22">Amylose</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: [Display omitted] • Raman spectroscopy was used to reveal the chemical composition of fungal spores. • In lipid-based spores, we determined chain length and unsaturation of triacylglycerols. • In protein-based spores, we determined the content of some amino acids and DNA. • The spores contain sugars (trehalose) and sometimes polysaccharides (amylose). • For larger spores, we built maps of key component distributions (proteins and lipids). Raman spectroscopy is a widely used technique for detecting various chemical compounds in organic matter and creating high-resolution, label-free maps on the level of individual cells. One of the main advantages of this technique is its ability to study samples in vivo without special pretreatment. However, it is rarely used to determine the ratios between different substances or to conduct in-depth quantitative analysis of the obtained spectra. In our study, we establish ratiometric equations that enable the estimation of mass concentrations of triacylglycerols, proteins, sugars, polysaccharides, and DNA. We demonstrate that it is possible to determine the average unsaturation and chain length from the spectra of lipids and concentrations of phenylalanine, tyrosine, and tryptophan from the spectra of proteins. We apply the derived equations to the Raman spectra of fungal spores from over 70 different species of macrofungi, providing a comprehensive characterization of the lipids, proteins, and polysaccharides present in the spores. We believe that our study not only contributes valuable fundamental knowledge to the field of mycology but also lays the groundwork for the spectral quantification of any organic material. So, our approach may be applicable in areas such as food diagnostics. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.saa.2025.125813 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Fungal proteins Type: general – SubjectFull: Fungal spores Type: general – SubjectFull: Organic compounds Type: general – SubjectFull: Saccharides Type: general – SubjectFull: Amylose Type: general Titles: – TitleFull: Quantitative evaluation of composition and biomolecular mapping of macrofungi spores by Raman spectroscopy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shvets, Petr – PersonEntity: Name: NameFull: Goikhman, Aleksandr IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 13861425 Numbering: – Type: volume Value: 331 Titles: – TitleFull: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy Type: main |
| ResultId | 1 |
