Evaluation of antimicrobial photodynamic therapy with erythrosine and blue light emitting diode for inactivation of Aggregatibacter actinomycetemcomitans.
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| Title: | Evaluation of antimicrobial photodynamic therapy with erythrosine and blue light emitting diode for inactivation of Aggregatibacter actinomycetemcomitans. |
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| Authors: | Latorre, Joaquin Isper1,2 (AUTHOR) joaquinisper@uni9.edu.br, Gonçalves, Marcela Letícia Leal3 (AUTHOR) marcelalleal@hotmail.com, González, Carolina Stéfani Wince1,2 (AUTHOR) caritowince@gmail.com, Kassa, Claudio Teruo2 (AUTHOR) claudiotkassa@hotmail.com, Rodriguez, Mikelhy Silva2,4 (AUTHOR) mikelhysr@gmail.com, Tortamano, Ana Carolina A. C.2 (AUTHOR) carolalves05@hotmail.com, Magalhães, Fabiana Divina2 (AUTHOR) fabi.magalhaes1@hotmail.com, Kato, Ilka Tiemy5 (AUTHOR) itkato@gmail.com, Bussadori, Sandra Kalil2 (AUTHOR) skb@gmail.com, Prates, Renato Araujo2 (AUTHOR) pratesra@gmail.com |
| Source: | Lasers in Medical Science. 4/14/2025, Vol. 40 Issue 1, p1-6. 6p. |
| Subjects: | Blue light emitting diodes, Actinobacillus actinomycetemcomitans, Blue light, Scanning electron microscopes, Photodynamic therapy |
| Abstract: | This study aims to analyze the effect of antimicrobial photodynamic therapy on Aggregatibacter actinomycetemcomitans using erythrosine as a photosensitizer and a blue light emitting-diode as a light source. Inoculum samples of A. actinomycetemcomitans with PBS were used in each of the groups, being the control group (C); light group (L) corresponding to light emitting-diode irradiation for 300 s; photosensitizing group (0) without irradiation; and the aPDT groups with different irradiation times (aPDT20) with 20s of irradiation; (aPDT40) with 40s of irradiation; (aPDT60) with 60s of irradiation; (aPDT180) with 180s; and (aPDT300) with 300s. Samples were used to determine colony forming units (CFU). Aliquots of 10 µL were plated through six serial dilutions on brain-heart infusion agar in Petri dishes. The plates were incubated at 37 °C for a period of up to 24–48 h under microaerophilic conditions to evaluate the total bacteria recovered. After this period, CFUs were counted, and the data was subjected to one-way analysis of variance. When aPDT was performed for 180 and 300 s, the mean log10 (CFU/ml) was equal to 0. In the aPDT60 group, a significant yet incomplete microbial reduction was observed. SEM images confirmed that membrane integrity was maintained, indicating that aPDT induced cellular alterations without causing membrane disruption. Antimicrobial photodynamic therapy employing erythrosine as a photosensitizer and blue light emitting-diode light-curing unit for composite resin polymerization used in dental practices demonstrated significant antimicrobial efficacy against A. actinomycetemcomitans, a principal pathogen in periodontitis, under the evaluated experimental conditions. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 184469792 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Evaluation of antimicrobial photodynamic therapy with erythrosine and blue light emitting diode for inactivation of Aggregatibacter actinomycetemcomitans. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Latorre%2C+Joaquin+Isper%22">Latorre, Joaquin Isper</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> joaquinisper@uni9.edu.br</i><br /><searchLink fieldCode="AR" term="%22Gonçalves%2C+Marcela+Letícia+Leal%22">Gonçalves, Marcela Letícia Leal</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> marcelalleal@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22González%2C+Carolina+Stéfani+Wince%22">González, Carolina Stéfani Wince</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> caritowince@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kassa%2C+Claudio+Teruo%22">Kassa, Claudio Teruo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> claudiotkassa@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Rodriguez%2C+Mikelhy+Silva%22">Rodriguez, Mikelhy Silva</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<i> mikelhysr@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Tortamano%2C+Ana+Carolina+A%2E+C%2E%22">Tortamano, Ana Carolina A. C.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> carolalves05@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Magalhães%2C+Fabiana+Divina%22">Magalhães, Fabiana Divina</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> fabi.magalhaes1@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Kato%2C+Ilka+Tiemy%22">Kato, Ilka Tiemy</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> itkato@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Bussadori%2C+Sandra+Kalil%22">Bussadori, Sandra Kalil</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> skb@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Prates%2C+Renato+Araujo%22">Prates, Renato Araujo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> pratesra@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 4/14/2025, Vol. 40 Issue 1, p1-6. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Blue+light+emitting+diodes%22">Blue light emitting diodes</searchLink><br /><searchLink fieldCode="DE" term="%22Actinobacillus+actinomycetemcomitans%22">Actinobacillus actinomycetemcomitans</searchLink><br /><searchLink fieldCode="DE" term="%22Blue+light%22">Blue light</searchLink><br /><searchLink fieldCode="DE" term="%22Scanning+electron+microscopes%22">Scanning electron microscopes</searchLink><br /><searchLink fieldCode="DE" term="%22Photodynamic+therapy%22">Photodynamic therapy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: This study aims to analyze the effect of antimicrobial photodynamic therapy on Aggregatibacter actinomycetemcomitans using erythrosine as a photosensitizer and a blue light emitting-diode as a light source. Inoculum samples of A. actinomycetemcomitans with PBS were used in each of the groups, being the control group (C); light group (L) corresponding to light emitting-diode irradiation for 300 s; photosensitizing group (0) without irradiation; and the aPDT groups with different irradiation times (aPDT20) with 20s of irradiation; (aPDT40) with 40s of irradiation; (aPDT60) with 60s of irradiation; (aPDT180) with 180s; and (aPDT300) with 300s. Samples were used to determine colony forming units (CFU). Aliquots of 10 µL were plated through six serial dilutions on brain-heart infusion agar in Petri dishes. The plates were incubated at 37 °C for a period of up to 24–48 h under microaerophilic conditions to evaluate the total bacteria recovered. After this period, CFUs were counted, and the data was subjected to one-way analysis of variance. When aPDT was performed for 180 and 300 s, the mean log10 (CFU/ml) was equal to 0. In the aPDT60 group, a significant yet incomplete microbial reduction was observed. SEM images confirmed that membrane integrity was maintained, indicating that aPDT induced cellular alterations without causing membrane disruption. Antimicrobial photodynamic therapy employing erythrosine as a photosensitizer and blue light emitting-diode light-curing unit for composite resin polymerization used in dental practices demonstrated significant antimicrobial efficacy against A. actinomycetemcomitans, a principal pathogen in periodontitis, under the evaluated experimental conditions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-025-04434-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 1 Subjects: – SubjectFull: Blue light emitting diodes Type: general – SubjectFull: Actinobacillus actinomycetemcomitans Type: general – SubjectFull: Blue light Type: general – SubjectFull: Scanning electron microscopes Type: general – SubjectFull: Photodynamic therapy Type: general Titles: – TitleFull: Evaluation of antimicrobial photodynamic therapy with erythrosine and blue light emitting diode for inactivation of Aggregatibacter actinomycetemcomitans. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Latorre, Joaquin Isper – PersonEntity: Name: NameFull: Gonçalves, Marcela Letícia Leal – PersonEntity: Name: NameFull: González, Carolina Stéfani Wince – PersonEntity: Name: NameFull: Kassa, Claudio Teruo – PersonEntity: Name: NameFull: Rodriguez, Mikelhy Silva – PersonEntity: Name: NameFull: Tortamano, Ana Carolina A. C. – PersonEntity: Name: NameFull: Magalhães, Fabiana Divina – PersonEntity: Name: NameFull: Kato, Ilka Tiemy – PersonEntity: Name: NameFull: Bussadori, Sandra Kalil – PersonEntity: Name: NameFull: Prates, Renato Araujo IsPartOfRelationships: – BibEntity: Dates: – D: 14 M: 04 Text: 4/14/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 40 – Type: issue Value: 1 Titles: – TitleFull: Lasers in Medical Science Type: main |
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