Evaluating the Chemical Reactivity of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding through Kendrick Mass Defect Analysis.
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| Title: | Evaluating the Chemical Reactivity of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding through Kendrick Mass Defect Analysis. |
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| Authors: | Hamontree, Hannah M.1 (AUTHOR), Hatcher, Patrick G.1 (AUTHOR) phatcher@odu.edu |
| Source: | Journal of the American Society for Mass Spectrometry. 6/4/2025, Vol. 36 Issue 36, p1377-1385. 9p. |
| Abstract: | The emerging risks to organisms of pyrogenic-derived dissolved organic matter (PyDOM) from forest fires are of concern due to its toxic and mutagenic potential (e.g., pro-oxidative responses in fauna through the depletion of glutathione, a nitrogen- and sulfur-containing tripeptide found in cells). This study simulates this phenomenon in a laboratory setting by identifying bonding between reduced l-glutathione and organic molecules in leachates from environmentally weathered biomass samples (charred and uncharred) using Kendrick Mass Defect (KMD) analysis from formula lists obtained from negative-mode electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry ((−)-ESI-FT-ICR-MS). These formula lists reveal a 10-fold increase in nitrogen- and sulfur-containing molecular formulas in the charred biomass samples compared with the unreacted charred biomass when subjected to reaction with glutathione. KMD analysis attributed the bonding of glutathione to the biomass leachates accounting for approximately 25% of the new nitrogen- and sulfur-containing molecular formulas as either addition-type or condensation/elimination-type reactions. KMD sheds light on a different fraction of chemically reactive wildfire-produced organic compounds that may be of interest for subsequent toxicological studies. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 185727020 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Evaluating the Chemical Reactivity of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding through Kendrick Mass Defect Analysis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hamontree%2C+Hannah+M%2E%22">Hamontree, Hannah M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hatcher%2C+Patrick+G%2E%22">Hatcher, Patrick G.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> phatcher@odu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. 6/4/2025, Vol. 36 Issue 36, p1377-1385. 9p. – Name: Abstract Label: Abstract Group: Ab Data: The emerging risks to organisms of pyrogenic-derived dissolved organic matter (PyDOM) from forest fires are of concern due to its toxic and mutagenic potential (e.g., pro-oxidative responses in fauna through the depletion of glutathione, a nitrogen- and sulfur-containing tripeptide found in cells). This study simulates this phenomenon in a laboratory setting by identifying bonding between reduced l-glutathione and organic molecules in leachates from environmentally weathered biomass samples (charred and uncharred) using Kendrick Mass Defect (KMD) analysis from formula lists obtained from negative-mode electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry ((−)-ESI-FT-ICR-MS). These formula lists reveal a 10-fold increase in nitrogen- and sulfur-containing molecular formulas in the charred biomass samples compared with the unreacted charred biomass when subjected to reaction with glutathione. KMD analysis attributed the bonding of glutathione to the biomass leachates accounting for approximately 25% of the new nitrogen- and sulfur-containing molecular formulas as either addition-type or condensation/elimination-type reactions. KMD sheds light on a different fraction of chemically reactive wildfire-produced organic compounds that may be of interest for subsequent toxicological studies. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/jasms.5c00077 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1377 Titles: – TitleFull: Evaluating the Chemical Reactivity of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding through Kendrick Mass Defect Analysis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hamontree, Hannah M. – PersonEntity: Name: NameFull: Hatcher, Patrick G. IsPartOfRelationships: – BibEntity: Dates: – D: 04 M: 06 Text: 6/4/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 10440305 Numbering: – Type: volume Value: 36 – Type: issue Value: 36 Titles: – TitleFull: Journal of the American Society for Mass Spectrometry Type: main |
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