A facile aptasensor based on Au dimers and carbon dots for SERS/fluorescence dual-mode detection of AFB1.
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| Title: | A facile aptasensor based on Au dimers and carbon dots for SERS/fluorescence dual-mode detection of AFB1. |
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| Authors: | Gao, Xueying1,2,3,4 (AUTHOR), Wang, Luning1,2,3,4 (AUTHOR), Chen, Yi1,2,3,4 (AUTHOR), Ma, Xi1,2,3,4 (AUTHOR), Hui, Minyi1,2,3,4 (AUTHOR), Wang, Zhouping1,2,3,4 (AUTHOR), Ma, Xiaoyuan1,2,3,4 (AUTHOR) maxy@jiangnan.edu.cn |
| Source: | Sensors & Actuators B: Chemical. Nov2025, Vol. 443, pN.PAG-N.PAG. 1p. |
| Subjects: | Aflatoxins, SERS spectroscopy, Fluorescence, Detection limit, Carbon nanodots, Gold nanoparticles, Empirical research, Biosensors |
| Abstract: | A dual-signal aptasensor that integrates Surface-Enhanced Raman Scattering (SERS) and fluorescence has been developed for the sensitive detection of aflatoxin B 1 (AFB 1). 2,2′-bipyridine ethylene (BPE) linked to gold nanoparticles (AuNPs) through "Au-N" bonds formed Au dimers with nanogaps. These were functionalized with complementary DNA (cDNA) of the AFB 1 apt, serving as both a Raman signal probe and a fluorescence quencher. The Fe 3 O 4 @SiO 2 -carbon dots (CDs), modified with the AFB 1 apt, functioned as a fluorescent magnetic substrate facilitating efficient separation. The components assembled into a satellite-like structure, which maximized SERS signals while simultaneously quenching fluorescence. Upon the addition of AFB 1 , the Raman probe was released, leading to a reduction in SERS signals and recovery of fluorescence. The sensor demonstrated an extensive linear detection range from 10⁻⁴ to 10² ng/mL, accompanied by an ultra-low limit of detection (LOD) of 0.066 pg/kg. Spiked recovery rates in peanut, rice, and corn samples ranged from 100.35 % to 109.75 %, indicating high accuracy and applicability for AFB 1 detection in real-world samples. [Display omitted] • Dual-signal AFB 1 detection was realized via LSPR, FRET, and FDQ integration. • Au dimers showed strong SERS enhancement and effective fluorescence quenching. • Fe 3 O 4 @SiO 2 -CDs enabled efficient separation and stable fluorescence. • The aptasensor exhibited high selectivity toward AFB 1. • AFB 1 detection limit reached 0.066 pg/mL, with high accuracy in real samples. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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