Design and implementation of a climate chamber for moisture sensitive nanotomography of biological samples.
Saved in:
| Title: | Design and implementation of a climate chamber for moisture sensitive nanotomography of biological samples. |
|---|---|
| Authors: | Nopens, Martin1 (AUTHOR) martin.nopens@thuenen.de, Greving, Imke2 (AUTHOR), Flenner, Silja2 (AUTHOR), Hesse, Linnea3,4 (AUTHOR), Lüdtke, Jan1 (AUTHOR), Altgen, Michael5 (AUTHOR), Koch, Gerald1 (AUTHOR), Beruda, Johannes3 (AUTHOR), Heldner, Sabrina1 (AUTHOR), Köhm, Hannes1 (AUTHOR), Kaschuro, Sergej1 (AUTHOR), Olbrich, Andrea1 (AUTHOR), Mietner, Jakob Benedikt3 (AUTHOR), Scheckenbach, Fabian3 (AUTHOR), Sieburg-Rockel, Jördis1 (AUTHOR), Krause, Andreas1 (AUTHOR) |
| Source: | Journal of Synchrotron Radiation. Sep2025, Vol. 32 Issue 5, p1354-1360. 7p. |
| Subjects: | Humidity, Tomography, Three-dimensional imaging, Phase-contrast microscopy, Biological specimens |
| Abstract: | Deep understanding of the structural composition and growth of biological specimens is becoming increasingly important for the development of bio‐based and sustainable material systems. Full‐field nano‐computed tomography is particularly suitable for this purpose as it allows for non‐destructive 3D imaging at high spatial resolution. However, most biological samples are functionalized by water and respond sensitively to any changes in climate conditions, specifically relative humidity, by adjusting their material moisture content. To date, only a limited number of tomography instruments offer an in situ climate control option to users. These, however, are limited either by the range of relative humidity states, the long times required to change the climate state, or obstruction or attenuation of the beam. Here, the first fully automatized climate cell for in situ full‐field nanotomography is presented. It has been designed, built and integrated at the nanotomography station at the P05 imaging beamline, operated by Hereon at the DESY storage ring PETRA III, Germany. The highly flexible and windowless design allows the humidity dependent swelling and shrinking of lignified plant cell walls to be studied in situ, using phase contrast nanotomography. The concept of this climate chamber can easily be integrated into other setups. It operates in the relative humidity range of 0–90% and can be controlled in a temperature range of 10–50°C. Climate conditions can be adjusted at any time, remotely from the control hutch by using a humidity generator. Results show that the developed setup maintains a stable climate during the entire duration of a tomographic scan at different humidities and does not obstruct the sample or hinder the imaging conditions. During the tomographic investigation the sample remains stable in the flow of the air stream and shows typical cell wall swelling and shrinking behaviour depending on the equilibrium moisture content. This new climate cell is now available to all users of the P05 nanotomography instrument for conditioning samples, serving a wide range of scientific applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Synchrotron Radiation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
Be the first to leave a comment!
