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The OsMPK9–OsCDR1 module regulates abiotic stress responses in rice.

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Title: The OsMPK9–OsCDR1 module regulates abiotic stress responses in rice.
Authors: Wang, Haitao1 (AUTHOR), Chen, Shijie1 (AUTHOR), Zhu, Wenjie1 (AUTHOR), Zhao, Ranran1 (AUTHOR), Wang, Enhe1 (AUTHOR), Gao, Xuanlin1 (AUTHOR), Jiang, Wenzhu1 (AUTHOR), Wu, Tao1 (AUTHOR) jidawt@jlu.edu.cn, Du, Xinglin1 (AUTHOR) duxinglin2004@163.com
Source: Plant Journal. Dec2025, Vol. 124 Issue 5, p1-16. 16p.
Subjects: Abiotic stress, Drought tolerance, Proteins, Abscisic acid, Thermal tolerance (Physiology), Rice, Plant breeding
Abstract: SUMMARY: Rice (Oryza sativa L.), originating from tropical and subtropical regions, is a cold‐sensitive and water‐demanding crop whose yield and quality are severely compromised by chilling injury or water deficits during growth and development. In this study, we systematically characterized Cold and Drought Resistance 1 (OsCDR1), a nucleus‐localized transcription activator belonging to the bZIP transcription factor family. OsCDR1 overexpression enhances rice tolerance to cold and drought stress, whereas knockout mutants of OsCDR1 exhibit reduced resistance to low temperatures and drought conditions. Furthermore, OsCDR1 positively regulates abscisic acid (ABA) signaling in rice, and mediates ABA‐regulated drought tolerance responses in rice. Integrated RNA‐seq and CUT&Tag analyses demonstrated that OsCDR1 coordinates with ABA‐dependent (PIP2;2) and ‐independent genes (DREB1J). OsCDR1 specifically binds to the G‐box cis‐element in the promoters of PIP2;2 and DREB1J, thereby activating their transcription and regulating the abiotic stress responses in rice. Protein interaction analysis revealed that OsCDR1 interacts with the kinase OsMPK9 in vivo and in vitro, and a dual‐luciferase reporter assay showed that OsMPK9 and OsCDR1 regulate the transcription of DREB1J and PIP2;2 in the same functional pathway. Overexpression of OsMPK9 inhibits ABA responses in rice and reduces cold and drought tolerance. Our findings establish the OsMPK9–OsCDR1 module as a critical hub connecting ABA signaling to abiotic stress resilience, providing new insights for breeding crops. Significance Statement: This study identifies the OsMPK9–OsCDR1 module as a critical integrator connecting abscisic acid signaling with abiotic stress tolerance in rice, thus providing a promising new genetic target for breeding climate‐resilient crops. [ABSTRACT FROM AUTHOR]
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 190280231
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: The OsMPK9–OsCDR1 module regulates abiotic stress responses in rice.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Haitao%22">Wang, Haitao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Shijie%22">Chen, Shijie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhu%2C+Wenjie%22">Zhu, Wenjie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhao%2C+Ranran%22">Zhao, Ranran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Enhe%22">Wang, Enhe</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gao%2C+Xuanlin%22">Gao, Xuanlin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jiang%2C+Wenzhu%22">Jiang, Wenzhu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Tao%22">Wu, Tao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jidawt@jlu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Du%2C+Xinglin%22">Du, Xinglin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> duxinglin2004@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Dec2025, Vol. 124 Issue 5, p1-16. 16p.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Abiotic+stress%22">Abiotic stress</searchLink><br /><searchLink fieldCode="DE" term="%22Drought+tolerance%22">Drought tolerance</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Abscisic+acid%22">Abscisic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Thermal+tolerance+%28Physiology%29%22">Thermal tolerance (Physiology)</searchLink><br /><searchLink fieldCode="DE" term="%22Rice%22">Rice</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+breeding%22">Plant breeding</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: SUMMARY: Rice (Oryza sativa L.), originating from tropical and subtropical regions, is a cold‐sensitive and water‐demanding crop whose yield and quality are severely compromised by chilling injury or water deficits during growth and development. In this study, we systematically characterized Cold and Drought Resistance 1 (OsCDR1), a nucleus‐localized transcription activator belonging to the bZIP transcription factor family. OsCDR1 overexpression enhances rice tolerance to cold and drought stress, whereas knockout mutants of OsCDR1 exhibit reduced resistance to low temperatures and drought conditions. Furthermore, OsCDR1 positively regulates abscisic acid (ABA) signaling in rice, and mediates ABA‐regulated drought tolerance responses in rice. Integrated RNA‐seq and CUT&Tag analyses demonstrated that OsCDR1 coordinates with ABA‐dependent (PIP2;2) and ‐independent genes (DREB1J). OsCDR1 specifically binds to the G‐box cis‐element in the promoters of PIP2;2 and DREB1J, thereby activating their transcription and regulating the abiotic stress responses in rice. Protein interaction analysis revealed that OsCDR1 interacts with the kinase OsMPK9 in vivo and in vitro, and a dual‐luciferase reporter assay showed that OsMPK9 and OsCDR1 regulate the transcription of DREB1J and PIP2;2 in the same functional pathway. Overexpression of OsMPK9 inhibits ABA responses in rice and reduces cold and drought tolerance. Our findings establish the OsMPK9–OsCDR1 module as a critical hub connecting ABA signaling to abiotic stress resilience, providing new insights for breeding crops. Significance Statement: This study identifies the OsMPK9–OsCDR1 module as a critical integrator connecting abscisic acid signaling with abiotic stress tolerance in rice, thus providing a promising new genetic target for breeding climate‐resilient crops. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=190280231
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/tpj.70627
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 16
        StartPage: 1
    Subjects:
      – SubjectFull: Abiotic stress
        Type: general
      – SubjectFull: Drought tolerance
        Type: general
      – SubjectFull: Proteins
        Type: general
      – SubjectFull: Abscisic acid
        Type: general
      – SubjectFull: Thermal tolerance (Physiology)
        Type: general
      – SubjectFull: Rice
        Type: general
      – SubjectFull: Plant breeding
        Type: general
    Titles:
      – TitleFull: The OsMPK9–OsCDR1 module regulates abiotic stress responses in rice.
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            NameFull: Wang, Haitao
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            NameFull: Chen, Shijie
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            NameFull: Zhu, Wenjie
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            NameFull: Zhao, Ranran
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            NameFull: Wang, Enhe
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            NameFull: Gao, Xuanlin
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            NameFull: Jiang, Wenzhu
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            NameFull: Wu, Tao
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            NameFull: Du, Xinglin
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            – D: 01
              M: 12
              Text: Dec2025
              Type: published
              Y: 2025
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