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A simple bacteria-mediated staining strategy: Ultrafast and high-resolution live-cell imaging with wash-free carbon dots.

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Title: A simple bacteria-mediated staining strategy: Ultrafast and high-resolution live-cell imaging with wash-free carbon dots.
Authors: Ma, Jianlong1 (AUTHOR), Gao, Wei1 (AUTHOR), Huo, Xiangyang1 (AUTHOR), He, Pinyi2 (AUTHOR), Bai, Jianliang1,2 (AUTHOR) 873106638@qq.com, Pei, Runfang1,3 (AUTHOR) 370543276@qq.com, Ren, Lili1,2 (AUTHOR) liliren@seu.edu.cn
Source: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Mar2026, Vol. 349, pN.PAG-N.PAG. 1p.
Subjects: Cell imaging, Stains & staining (Microscopy), Carbon nanodots, Imaging systems, Cell analysis
Abstract: Nucleus labeling is significant to dynamic monitor many physiological and pathophysiological processes. In the current work, a nuclear-targeted carbon dots (named as mf -CDs) was synthesized from m -phenylenediamine and folic acid through the hydrothermal method. The prepared mf -CDs were water-soluble and could specifically "light up" the nucleus whether it's normal cells or cancer cells. Remarkably, this staining process is both ultrafast and wash-free. The universality of mf -CDs was demonstrated through successful imaging across diverse cell systems. Including bacterial species such as Escherichia coli and Nitrogen fixing bacteria , fungi like Rhizoctonia solani and Saccharomyces cerevisiae , as well as plant cells from onion epidermis. Notably, excellent nuclear specificity was observed in Saccharomyces cerevisiae and onion epidermal cells. More interestingly, the Escherichia coli or Saccharomyces cerevisiae preloaded with low concentration (3.0 μg/mL) of mf -CDs effectively stained live HepG2 cells, high-resolution images fluorescence image was obtained within 4 min. Revealing that mf -CDs could ultrafast label the living cells under extremely low concentration conditions. To the best of our knowledge, this novel bacteria-mediated staining process has not been previously reported. The current work advances ultralow-concentration CDs bioimaging and pioneers microbial-mediated cellular analysis, offering new tools to study nanoparticle-cell interactions. [Display omitted] • A carbon dots (mf -CDs) was synthesized through the hydrothermal method, the prepared mf -CDs could specifically light up the cell nucleus within 30 s. • mf -CDs could penetrate the complex cell wall to achieve labeling of bacterial, fungi and plant cells, respectively. • Bacterial or fungi which loaded with mf -CDs was successfully stained live HepG2 cells precise fluorescent image was obtained swiftly. • Using bacteria-mediated, mf -CDs could ultrafast label the living cells under extremely low concentration conditions. [ABSTRACT FROM AUTHOR]
Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: A simple bacteria-mediated staining strategy: Ultrafast and high-resolution live-cell imaging with wash-free carbon dots.
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  Data: <searchLink fieldCode="AR" term="%22Ma%2C+Jianlong%22">Ma, Jianlong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gao%2C+Wei%22">Gao, Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huo%2C+Xiangyang%22">Huo, Xiangyang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22He%2C+Pinyi%22">He, Pinyi</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bai%2C+Jianliang%22">Bai, Jianliang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> 873106638@qq.com</i><br /><searchLink fieldCode="AR" term="%22Pei%2C+Runfang%22">Pei, Runfang</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> 370543276@qq.com</i><br /><searchLink fieldCode="AR" term="%22Ren%2C+Lili%22">Ren, Lili</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> liliren@seu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Mar2026, Vol. 349, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Cell+imaging%22">Cell imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Stains+%26+staining+%28Microscopy%29%22">Stains & staining (Microscopy)</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+nanodots%22">Carbon nanodots</searchLink><br /><searchLink fieldCode="DE" term="%22Imaging+systems%22">Imaging systems</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+analysis%22">Cell analysis</searchLink>
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  Label: Abstract
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  Data: Nucleus labeling is significant to dynamic monitor many physiological and pathophysiological processes. In the current work, a nuclear-targeted carbon dots (named as mf -CDs) was synthesized from m -phenylenediamine and folic acid through the hydrothermal method. The prepared mf -CDs were water-soluble and could specifically "light up" the nucleus whether it's normal cells or cancer cells. Remarkably, this staining process is both ultrafast and wash-free. The universality of mf -CDs was demonstrated through successful imaging across diverse cell systems. Including bacterial species such as Escherichia coli and Nitrogen fixing bacteria , fungi like Rhizoctonia solani and Saccharomyces cerevisiae , as well as plant cells from onion epidermis. Notably, excellent nuclear specificity was observed in Saccharomyces cerevisiae and onion epidermal cells. More interestingly, the Escherichia coli or Saccharomyces cerevisiae preloaded with low concentration (3.0 μg/mL) of mf -CDs effectively stained live HepG2 cells, high-resolution images fluorescence image was obtained within 4 min. Revealing that mf -CDs could ultrafast label the living cells under extremely low concentration conditions. To the best of our knowledge, this novel bacteria-mediated staining process has not been previously reported. The current work advances ultralow-concentration CDs bioimaging and pioneers microbial-mediated cellular analysis, offering new tools to study nanoparticle-cell interactions. [Display omitted] • A carbon dots (mf -CDs) was synthesized through the hydrothermal method, the prepared mf -CDs could specifically light up the cell nucleus within 30 s. • mf -CDs could penetrate the complex cell wall to achieve labeling of bacterial, fungi and plant cells, respectively. • Bacterial or fungi which loaded with mf -CDs was successfully stained live HepG2 cells precise fluorescent image was obtained swiftly. • Using bacteria-mediated, mf -CDs could ultrafast label the living cells under extremely low concentration conditions. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.saa.2025.127274
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Cell imaging
        Type: general
      – SubjectFull: Stains & staining (Microscopy)
        Type: general
      – SubjectFull: Carbon nanodots
        Type: general
      – SubjectFull: Imaging systems
        Type: general
      – SubjectFull: Cell analysis
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      – TitleFull: A simple bacteria-mediated staining strategy: Ultrafast and high-resolution live-cell imaging with wash-free carbon dots.
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            NameFull: Ma, Jianlong
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            NameFull: Gao, Wei
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            NameFull: Huo, Xiangyang
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            NameFull: Bai, Jianliang
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            NameFull: Pei, Runfang
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            NameFull: Ren, Lili
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            – D: 18
              M: 03
              Text: Mar2026
              Type: published
              Y: 2026
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              Value: 349
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