Hydration State Throughout Porcine Lumbar Intervertebral Discs: Comparing Fresh and Frozen-Thawed Specimens.
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| Title: | Hydration State Throughout Porcine Lumbar Intervertebral Discs: Comparing Fresh and Frozen-Thawed Specimens. |
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| Authors: | Morino, Concetta1,2,3 (AUTHOR) concettamorino@gmail.com, Kait, Jason2 (AUTHOR), Bass, Cameron R.1,2,4 (AUTHOR) |
| Source: | Annals of Biomedical Engineering. Apr2026, Vol. 54 Issue 4, p1068-1075. 8p. |
| Subjects: | Moisture, Intervertebral disk, Tissue mechanics, Nucleus pulposus, Lumbar vertebrae |
| Abstract: | Water content in intervertebral discs (IVDs) is essential for physiological and mechanical function. Freezing post-mortem tissue prior to biomechanical testing is a common practice to prevent tissue degradation, but this process has been theorized to alter hydration within IVDs. The hydration state throughout porcine lumbar IVDs, a common lumbar surrogate, is unknown as are the effects of freezing on porcine IVD hydration. Nineteen porcine lumbar spines were stored in one of the three conditions: frozen (− 20 °C) wrapped in saline-soaked gauze, frozen (− 20 °C) without saline, or fresh. Water content was measured in four disc regions within each of 89 discs: nucleus pulposus (NP), inner (AF-A), intermediate (AF-B), and outer (AF-C) annulus fibrosus. A three-factor, repeated measure analysis of variance was conducted for storage condition, spinal level, and repeated measure disc region. No significant differences were observed in spinal level or storage condition as a main effect. Mean hydration was significantly different in each disc region with mass percentage of water found to be 88.8 ± 1.7% in NP, 79.6 ± 3.8% in AF-A, 71.9 ± 3.7% in AF-B, and 62.3 ± 3.3% in AF-C. No significant differences were shown in NP and AF-C regions between storage conditions. Two significant differences in storage condition were observed in AF-A and AF-B regions, but there is likely no biological difference in these populations. Water content throughout porcine lumbar IVD was determined and results suggest one freeze-thaw cycle at − 20 °C does not alter the overall hydration within the porcine lumbar IVD. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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