Antibody-Based Monitoring of Nitroaromatic Explosive Contaminants in the Environment.
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| Title: | Antibody-Based Monitoring of Nitroaromatic Explosive Contaminants in the Environment. |
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| Authors: | Zelenović, Nevena1 (AUTHOR), Simonović, Mladen2 (AUTHOR), Popović, Milica3 (AUTHOR) la_bioquimica@chem.bg.ac.rs |
| Source: | Journal of Analytical Chemistry. Apr2026, Vol. 81 Issue 4, p614-627. 14p. |
| Subjects: | Nitroaromatic compounds, Environmental monitoring, Monoclonal antibodies, Explosives detection, Immunoassay, Biosensors, Environmental toxicology, Recombinant antibodies |
| Abstract: | Nitroaromatic compounds (NACs), which are frequently used as explosives, represent a class of persistent xenobiotics with considerable biochemical and ecological relevance. Their detection in complex matrices such as soil and water represents a major analytical challenge. Although chromatographic and mass spectrometric methods offer high sensitivity, their applicability is limited by demanding sample preparation and instrumentation. In recent years, immunochemical methods have established themselves as highly selective bioanalytical tools for the detection of trace amounts of NACs. This review highlights the structural principles of hapten-antibody interactions, the production and characterization of monoclonal and recombinant antibodies, and their application in immunoassays and lateral flow devices. A particular focus is placed on the development of antibody fragments and nanobodies that offer improved thermal and chemical stability, reduced steric hindrance, and compatibility with biosensor technologies. The integration of immunoassay formats with transducer platforms is discussed in the context of the further development of field-ready diagnostics. Immunodetection is thus an example of a bioanalytical strategy that combines molecular detection with biochemical assay design and represents a robust alternative for monitoring environmental pollutants of toxicological concern. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Analytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 193493949 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Antibody-Based Monitoring of Nitroaromatic Explosive Contaminants in the Environment. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zelenović%2C+Nevena%22">Zelenović, Nevena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Simonović%2C+Mladen%22">Simonović, Mladen</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Popović%2C+Milica%22">Popović, Milica</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> la_bioquimica@chem.bg.ac.rs</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Analytical+Chemistry%22">Journal of Analytical Chemistry</searchLink>. Apr2026, Vol. 81 Issue 4, p614-627. 14p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Nitroaromatic+compounds%22">Nitroaromatic compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Environmental+monitoring%22">Environmental monitoring</searchLink><br /><searchLink fieldCode="DE" term="%22Monoclonal+antibodies%22">Monoclonal antibodies</searchLink><br /><searchLink fieldCode="DE" term="%22Explosives+detection%22">Explosives detection</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoassay%22">Immunoassay</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Environmental+toxicology%22">Environmental toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Recombinant+antibodies%22">Recombinant antibodies</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Nitroaromatic compounds (NACs), which are frequently used as explosives, represent a class of persistent xenobiotics with considerable biochemical and ecological relevance. Their detection in complex matrices such as soil and water represents a major analytical challenge. Although chromatographic and mass spectrometric methods offer high sensitivity, their applicability is limited by demanding sample preparation and instrumentation. In recent years, immunochemical methods have established themselves as highly selective bioanalytical tools for the detection of trace amounts of NACs. This review highlights the structural principles of hapten-antibody interactions, the production and characterization of monoclonal and recombinant antibodies, and their application in immunoassays and lateral flow devices. A particular focus is placed on the development of antibody fragments and nanobodies that offer improved thermal and chemical stability, reduced steric hindrance, and compatibility with biosensor technologies. The integration of immunoassay formats with transducer platforms is discussed in the context of the further development of field-ready diagnostics. Immunodetection is thus an example of a bioanalytical strategy that combines molecular detection with biochemical assay design and represents a robust alternative for monitoring environmental pollutants of toxicological concern. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Analytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1134/S1061934825602427 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 614 Subjects: – SubjectFull: Nitroaromatic compounds Type: general – SubjectFull: Environmental monitoring Type: general – SubjectFull: Monoclonal antibodies Type: general – SubjectFull: Explosives detection Type: general – SubjectFull: Immunoassay Type: general – SubjectFull: Biosensors Type: general – SubjectFull: Environmental toxicology Type: general – SubjectFull: Recombinant antibodies Type: general Titles: – TitleFull: Antibody-Based Monitoring of Nitroaromatic Explosive Contaminants in the Environment. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zelenović, Nevena – PersonEntity: Name: NameFull: Simonović, Mladen – PersonEntity: Name: NameFull: Popović, Milica IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 10619348 Numbering: – Type: volume Value: 81 – Type: issue Value: 4 Titles: – TitleFull: Journal of Analytical Chemistry Type: main |
| ResultId | 1 |
