Understanding time-growth rate relationship for slow-growing microbial cells in retentostat culturing systems.
Saved in:
| Title: | Understanding time-growth rate relationship for slow-growing microbial cells in retentostat culturing systems. |
|---|---|
| Authors: | Aranda, Beatriz1 (AUTHOR), Gonzalez, Juan M.1 (AUTHOR) juan.gonzalez@csic.es |
| Source: | Applied Microbiology & Biotechnology. 4/25/2026, Vol. 110 Issue 1, p1-9. 9p. |
| Subjects: | Microbial growth, Cell growth, Microbial cultures, Prokaryotes |
| Abstract: | Generally, microorganisms in nature are severely growth-limited because of nutrient availability, competition, or physico-chemical parameters. To simulate these growing conditions, the use of continuous culturing systems has been proposed. One modification of these systems is the so-called retentostat. It corresponds to a system closed to cells and open to nutrients, and a nutrient is limiting growth. In this system, cells are forced to reduce progressively their growth rate as a result of increasing biomass sharing the same resources. In these systems, near-zero growth rates (corresponding to long doubling times) can be achieved within relatively reasonable time frames. Nevertheless, in these systems, the relationship between growth rate and time remains to be further analyzed. Herein, we evaluate a retentostat culturing system for different prokaryotic species. We established a general relationship between growth rate and incubation time. This allows us to easily estimate either the growth rate reached after a given incubation time or the time required to obtain cells at a specific slow growth rate. The results suggested progressive adaptation of cells at decreasing growth rates. This contribution simplifies estimates for incubation time-dependent growth rates and shows a retentostat as a species-independent culturing system where growth rates depend on time and dilution rate. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 194394897 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Understanding time-growth rate relationship for slow-growing microbial cells in retentostat culturing systems. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Aranda%2C+Beatriz%22">Aranda, Beatriz</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gonzalez%2C+Juan+M%2E%22">Gonzalez, Juan M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> juan.gonzalez@csic.es</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 4/25/2026, Vol. 110 Issue 1, p1-9. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Microbial+growth%22">Microbial growth</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+growth%22">Cell growth</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+cultures%22">Microbial cultures</searchLink><br /><searchLink fieldCode="DE" term="%22Prokaryotes%22">Prokaryotes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Generally, microorganisms in nature are severely growth-limited because of nutrient availability, competition, or physico-chemical parameters. To simulate these growing conditions, the use of continuous culturing systems has been proposed. One modification of these systems is the so-called retentostat. It corresponds to a system closed to cells and open to nutrients, and a nutrient is limiting growth. In this system, cells are forced to reduce progressively their growth rate as a result of increasing biomass sharing the same resources. In these systems, near-zero growth rates (corresponding to long doubling times) can be achieved within relatively reasonable time frames. Nevertheless, in these systems, the relationship between growth rate and time remains to be further analyzed. Herein, we evaluate a retentostat culturing system for different prokaryotic species. We established a general relationship between growth rate and incubation time. This allows us to easily estimate either the growth rate reached after a given incubation time or the time required to obtain cells at a specific slow growth rate. The results suggested progressive adaptation of cells at decreasing growth rates. This contribution simplifies estimates for incubation time-dependent growth rates and shows a retentostat as a species-independent culturing system where growth rates depend on time and dilution rate. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=194394897 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-026-13817-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: Microbial growth Type: general – SubjectFull: Cell growth Type: general – SubjectFull: Microbial cultures Type: general – SubjectFull: Prokaryotes Type: general Titles: – TitleFull: Understanding time-growth rate relationship for slow-growing microbial cells in retentostat culturing systems. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Aranda, Beatriz – PersonEntity: Name: NameFull: Gonzalez, Juan M. IsPartOfRelationships: – BibEntity: Dates: – D: 25 M: 04 Text: 4/25/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 110 – Type: issue Value: 1 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
