Sturdier DNA Nanotubes via Ligation.
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| Title: | Sturdier DNA Nanotubes via Ligation. |
|---|---|
| Authors: | Patrick O'Neill1, Paul W. K. Rothemund1, Ashish Kumar1, D. K. Fygenson1 |
| Source: | Nano Letters. Jul2006, Vol. 6 Issue 7, p1379-1383. 5p. |
| Subjects: | DNA, Nanotubes, DNA ligases, Atomic force microscopy |
| Abstract: | DNA nanotubes are crystalline self-assemblies of DNA tiles ∼10 nm in diameter that readily grow tens of micrometers in length. Easy assembly, programmability, and stiffness make them interesting for many applications, but DNA nanotubes begin to melt at temperatures below 40 °C, break open when deposited on mica or scanned by AFM, and disintegrate in deionized water. These weaknesses can be traced to the presence of discontinuities in the phosphate backbone, called nicks. The nanotubes studied here have five nicks, one in the core of a tile and one at each corner. We report the successful ligation of all four corner nicks by T4 DNA ligase. Although ligation does not change the nanotubes' stiffness, ligated nanotubes withstand temperatures over 70 °C, resist breaking during AFM, and are stable in pure water for over a month. Ligated DNA nanotubes are thus physically and chemically sturdy enough to withstand the manipulations necessary for many technological applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Nano Letters is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 21630264 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/nl0603505 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 1379 Subjects: – SubjectFull: DNA Type: general – SubjectFull: Nanotubes Type: general – SubjectFull: DNA ligases Type: general – SubjectFull: Atomic force microscopy Type: general Titles: – TitleFull: Sturdier DNA Nanotubes via Ligation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Patrick O'Neill – PersonEntity: Name: NameFull: Paul W. K. Rothemund – PersonEntity: Name: NameFull: Ashish Kumar – PersonEntity: Name: NameFull: D. K. Fygenson IsPartOfRelationships: – BibEntity: Dates: – D: 12 M: 07 Text: Jul2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 15306984 Numbering: – Type: volume Value: 6 – Type: issue Value: 7 Titles: – TitleFull: Nano Letters Type: main |
| ResultId | 1 |
