Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms
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| Title: | Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms |
|---|---|
| Authors: | Zacco, E.1, Galve, R.1, Marco, M.P.2, Alegret, S.1 salvador.alegret@uab.es, Pividori, M.I.1 |
| Source: | Biosensors & Bioelectronics. Mar2007, Vol. 22 Issue 8, p1707-1715. 9p. |
| Subjects: | Electrochemical sensors, Biosensors, Pesticide residues in food, Atrazine |
| Abstract: | Abstract: A novel and very sensitive electrochemical immunosensing strategy for the detection of atrazine based on affinity biocomposite transducers is presented. Firstly, the graphite–epoxy composite transducer was bulk-modified with different universal affinity biomolecules, such as avidin and Protein A. Two strategies for the immobilization of the anti-atrazine antibodies on both biocomposite transducers were evaluated: ‘wet-affinity’ and ‘dry-assisted affinity’ immobilization. Finally, the performance of a novel anti-atrazine immunocomposite bulk-modified with anti-atrazine antibodies was also evaluated. The better immobilization performance of the anti-atrazine antibodies was achieved by ‘dry-assisted affinity’ immobilization on Protein A (2%) graphite–epoxy biocomposite (ProtA(2%)-GEB) as a transducer. The immunological reaction for the detection of atrazine performed on the ProtA(2%)-GEB biosensors is based on a direct competitive assay using atrazine-HRP tracer as the enzymatic label. The electrochemical detection is thus achieved through a suitable substrate and a mediator for the enzyme HRP. This novel strategy was successfully evaluated using spiked orange juice samples. The detection limit for atrazine in orange juices using the competitive electrochemical immunosensing assay was found to be 6×10−3 μgL−1 (0.03nmolL−1) thus this biosensing method accomplishes by far the LODs required for the European Community directives for potable water and food samples (0.1μgL−1). This strategy offers great promise for rapid, simple, cost effective, and on-site biosensing of biological, food, and environmental samples. [Copyright &y& Elsevier] |
| Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 23866152 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zacco%2C+E%2E%22">Zacco, E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Galve%2C+R%2E%22">Galve, R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Marco%2C+M%2EP%2E%22">Marco, M.P.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Alegret%2C+S%2E%22">Alegret, S.</searchLink><relatesTo>1</relatesTo><i> salvador.alegret@uab.es</i><br /><searchLink fieldCode="AR" term="%22Pividori%2C+M%2EI%2E%22">Pividori, M.I.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. Mar2007, Vol. 22 Issue 8, p1707-1715. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Electrochemical+sensors%22">Electrochemical sensors</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Pesticide+residues+in+food%22">Pesticide residues in food</searchLink><br /><searchLink fieldCode="DE" term="%22Atrazine%22">Atrazine</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: A novel and very sensitive electrochemical immunosensing strategy for the detection of atrazine based on affinity biocomposite transducers is presented. Firstly, the graphite–epoxy composite transducer was bulk-modified with different universal affinity biomolecules, such as avidin and Protein A. Two strategies for the immobilization of the anti-atrazine antibodies on both biocomposite transducers were evaluated: ‘wet-affinity’ and ‘dry-assisted affinity’ immobilization. Finally, the performance of a novel anti-atrazine immunocomposite bulk-modified with anti-atrazine antibodies was also evaluated. The better immobilization performance of the anti-atrazine antibodies was achieved by ‘dry-assisted affinity’ immobilization on Protein A (2%) graphite–epoxy biocomposite (ProtA(2%)-GEB) as a transducer. The immunological reaction for the detection of atrazine performed on the ProtA(2%)-GEB biosensors is based on a direct competitive assay using atrazine-HRP tracer as the enzymatic label. The electrochemical detection is thus achieved through a suitable substrate and a mediator for the enzyme HRP. This novel strategy was successfully evaluated using spiked orange juice samples. The detection limit for atrazine in orange juices using the competitive electrochemical immunosensing assay was found to be 6×10−3 μgL−1 (0.03nmolL−1) thus this biosensing method accomplishes by far the LODs required for the European Community directives for potable water and food samples (0.1μgL−1). This strategy offers great promise for rapid, simple, cost effective, and on-site biosensing of biological, food, and environmental samples. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.bios.2006.07.037 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1707 Subjects: – SubjectFull: Electrochemical sensors Type: general – SubjectFull: Biosensors Type: general – SubjectFull: Pesticide residues in food Type: general – SubjectFull: Atrazine Type: general Titles: – TitleFull: Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zacco, E. – PersonEntity: Name: NameFull: Galve, R. – PersonEntity: Name: NameFull: Marco, M.P. – PersonEntity: Name: NameFull: Alegret, S. – PersonEntity: Name: NameFull: Pividori, M.I. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 03 Text: Mar2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 09565663 Numbering: – Type: volume Value: 22 – Type: issue Value: 8 Titles: – TitleFull: Biosensors & Bioelectronics Type: main |
| ResultId | 1 |
