View in EDS PDF Full Text

Fine mapping and DNA fiber FISH analysis locates the tobamovirus resistance gene L 3 of Capsicum chinense in a 400-kb region of R-like genes cluster embedded in highly repetitive sequences.

Saved in:
Bibliographic Details
Title: Fine mapping and DNA fiber FISH analysis locates the tobamovirus resistance gene L 3 of Capsicum chinense in a 400-kb region of R-like genes cluster embedded in highly repetitive sequences.
Authors: Tomita, R.1, Murai, J.2, Miura, Y.3, Ishihara, H.4, Liu, S.5,6, Kubotera, Y.1, Honda, A.1, Hatta, R.1, Kuroda, T.7, Hamada, H.8, Sakamoto, M.1, Munemura, I.1, Nunomura, O.6, Ishikawa, K.6, Genda, Y.6, Kawasaki, S.9, Suzuki, K.10, Meksem, K.5, Kobayashi, K.1 kappei@ibrc.or.jp
Source: Theoretical & Applied Genetics. Nov2008, Vol. 117 Issue 7, p1107-1118. 12p. 1 Color Photograph, 1 Black and White Photograph, 2 Diagrams, 2 Charts.
Subjects: Population genetics, Cultivars, Plant genetic engineering, Plant breeding, Biomarkers
Abstract: The tobamovirus resistance gene L 3 of Capsicum chinense was mapped using an intra-specific F2 population (2,016 individuals) of Capsicum annuum cultivars, into one of which had been introduced the C. chinense L 3 gene, and an inter-specific F2 population (3,391 individuals) between C. chinense and Capsicum frutescence. Analysis of a BAC library with an AFLP marker closely linked to L 3-resistance revealed the presence of homologs of the tomato disease resistance gene I2. Partial or full-length coding sequences were cloned by degenerate PCR from 35 different pepper I2 homologs and 17 genetic markers were generated in the inter-specific combination. The L 3 gene was mapped between I2 homolog marker IH1-04 and BAC-end marker 189D23M, and located within a region encompassing two different BAC contigs consisting of four and one clones, respectively. DNA fiber FISH analysis revealed that these two contigs are separated from each other by about 30 kb. DNA fiber FISH results and Southern blotting of the BAC clones suggested that the L 3 locus-containing region is rich in highly repetitive sequences. Southern blot analysis indicated that the two BAC contigs contain more than ten copies of the I2 homologs. In contrast to the inter-specific F2 population, no recombinant progeny were identified to have a crossover point within two BAC contigs consisting of seven and two clones in the intra-specific F2 population. Moreover, distribution of the crossover points differed between the two populations, suggesting linkage disequilibrium in the region containing the L locus. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 34729499
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Fine mapping and DNA fiber FISH analysis locates the tobamovirus resistance gene L <superscript>3</superscript> of Capsicum chinense in a 400-kb region of R-like genes cluster embedded in highly repetitive sequences.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Tomita%2C+R%2E%22">Tomita, R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Murai%2C+J%2E%22">Murai, J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Miura%2C+Y%2E%22">Miura, Y.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ishihara%2C+H%2E%22">Ishihara, H.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+S%2E%22">Liu, S.</searchLink><relatesTo>5,6</relatesTo><br /><searchLink fieldCode="AR" term="%22Kubotera%2C+Y%2E%22">Kubotera, Y.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Honda%2C+A%2E%22">Honda, A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hatta%2C+R%2E%22">Hatta, R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kuroda%2C+T%2E%22">Kuroda, T.</searchLink><relatesTo>7</relatesTo><br /><searchLink fieldCode="AR" term="%22Hamada%2C+H%2E%22">Hamada, H.</searchLink><relatesTo>8</relatesTo><br /><searchLink fieldCode="AR" term="%22Sakamoto%2C+M%2E%22">Sakamoto, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Munemura%2C+I%2E%22">Munemura, I.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nunomura%2C+O%2E%22">Nunomura, O.</searchLink><relatesTo>6</relatesTo><br /><searchLink fieldCode="AR" term="%22Ishikawa%2C+K%2E%22">Ishikawa, K.</searchLink><relatesTo>6</relatesTo><br /><searchLink fieldCode="AR" term="%22Genda%2C+Y%2E%22">Genda, Y.</searchLink><relatesTo>6</relatesTo><br /><searchLink fieldCode="AR" term="%22Kawasaki%2C+S%2E%22">Kawasaki, S.</searchLink><relatesTo>9</relatesTo><br /><searchLink fieldCode="AR" term="%22Suzuki%2C+K%2E%22">Suzuki, K.</searchLink><relatesTo>10</relatesTo><br /><searchLink fieldCode="AR" term="%22Meksem%2C+K%2E%22">Meksem, K.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Kobayashi%2C+K%2E%22">Kobayashi, K.</searchLink><relatesTo>1</relatesTo><i> kappei@ibrc.or.jp</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Nov2008, Vol. 117 Issue 7, p1107-1118. 12p. 1 Color Photograph, 1 Black and White Photograph, 2 Diagrams, 2 Charts.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Population+genetics%22">Population genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Cultivars%22">Cultivars</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetic+engineering%22">Plant genetic engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+breeding%22">Plant breeding</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The tobamovirus resistance gene L 3 of Capsicum chinense was mapped using an intra-specific F2 population (2,016 individuals) of Capsicum annuum cultivars, into one of which had been introduced the C. chinense L 3 gene, and an inter-specific F2 population (3,391 individuals) between C. chinense and Capsicum frutescence. Analysis of a BAC library with an AFLP marker closely linked to L 3-resistance revealed the presence of homologs of the tomato disease resistance gene I2. Partial or full-length coding sequences were cloned by degenerate PCR from 35 different pepper I2 homologs and 17 genetic markers were generated in the inter-specific combination. The L 3 gene was mapped between I2 homolog marker IH1-04 and BAC-end marker 189D23M, and located within a region encompassing two different BAC contigs consisting of four and one clones, respectively. DNA fiber FISH analysis revealed that these two contigs are separated from each other by about 30 kb. DNA fiber FISH results and Southern blotting of the BAC clones suggested that the L 3 locus-containing region is rich in highly repetitive sequences. Southern blot analysis indicated that the two BAC contigs contain more than ten copies of the I2 homologs. In contrast to the inter-specific F2 population, no recombinant progeny were identified to have a crossover point within two BAC contigs consisting of seven and two clones in the intra-specific F2 population. Moreover, distribution of the crossover points differed between the two populations, suggesting linkage disequilibrium in the region containing the L locus. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=34729499
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s00122-008-0848-6
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 1107
    Subjects:
      – SubjectFull: Population genetics
        Type: general
      – SubjectFull: Cultivars
        Type: general
      – SubjectFull: Plant genetic engineering
        Type: general
      – SubjectFull: Plant breeding
        Type: general
      – SubjectFull: Biomarkers
        Type: general
    Titles:
      – TitleFull: Fine mapping and DNA fiber FISH analysis locates the tobamovirus resistance gene L 3 of Capsicum chinense in a 400-kb region of R-like genes cluster embedded in highly repetitive sequences.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Tomita, R.
      – PersonEntity:
          Name:
            NameFull: Murai, J.
      – PersonEntity:
          Name:
            NameFull: Miura, Y.
      – PersonEntity:
          Name:
            NameFull: Ishihara, H.
      – PersonEntity:
          Name:
            NameFull: Liu, S.
      – PersonEntity:
          Name:
            NameFull: Kubotera, Y.
      – PersonEntity:
          Name:
            NameFull: Honda, A.
      – PersonEntity:
          Name:
            NameFull: Hatta, R.
      – PersonEntity:
          Name:
            NameFull: Kuroda, T.
      – PersonEntity:
          Name:
            NameFull: Hamada, H.
      – PersonEntity:
          Name:
            NameFull: Sakamoto, M.
      – PersonEntity:
          Name:
            NameFull: Munemura, I.
      – PersonEntity:
          Name:
            NameFull: Nunomura, O.
      – PersonEntity:
          Name:
            NameFull: Ishikawa, K.
      – PersonEntity:
          Name:
            NameFull: Genda, Y.
      – PersonEntity:
          Name:
            NameFull: Kawasaki, S.
      – PersonEntity:
          Name:
            NameFull: Suzuki, K.
      – PersonEntity:
          Name:
            NameFull: Meksem, K.
      – PersonEntity:
          Name:
            NameFull: Kobayashi, K.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 11
              Text: Nov2008
              Type: published
              Y: 2008
          Identifiers:
            – Type: issn-print
              Value: 00405752
          Numbering:
            – Type: volume
              Value: 117
            – Type: issue
              Value: 7
          Titles:
            – TitleFull: Theoretical & Applied Genetics
              Type: main
ResultId 1