Assessment of olive-mill wastewater as a growth medium for lipase production by Candida cylindracea in bench-top reactor
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| Title: | Assessment of olive-mill wastewater as a growth medium for lipase production by Candida cylindracea in bench-top reactor |
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| Authors: | Brozzoli, V.1, Crognale, S.1, Sampedro, I.1,2, Federici, F.1, D’Annibale, A.1, Petruccioli, M.1 petrucci@unitus.it |
| Source: | Bioresource Technology. Jul2009, Vol. 100 Issue 13, p3395-3402. 8p. |
| Subjects: | Olive industry, Wastewater treatment, Industrial wastes, Waste products, Lipases, Candida |
| Abstract: | Olive-mill wastewater (OMW) was investigated for its suitability to serve as a medium for lipase production by Candida cylindracea NRRL Y-17506. The OMW that best supported enzyme production was characterized by low COD and low total sugars content. In shake flask batch cultures, OMW supplementation with 2.4gl−1 NH4Cl and 3gl−1 olive oil led to an enzyme activity of about 10Uml−1. The addition of glucose or malt extract and supplements containing organic N (e.g., peptone, yeast extract) either depressed or did not affect the enzyme production. Further experiments were then performed in a 3-l stirred tank reactor to assess the impact of medium pH and stirring speed on the yeast enzyme activity. The lipase activity was low (1.8Uml−1) when the pH was held constant at 6.5, significantly increased (18.7Uml−1) with uncontrolled pH and was maximum (20.4Uml−1) when the pH was let free to vary below 6.5. A stirring regime, that varied depending on the dissolved oxygen concentration in the medium, both prevented the occurrence of anoxic conditions during the exponential growth phase and enabled good lipase production (i.e., 21.6Uml−1) and mean volumetric productivity (i.e., 123.5 Ul−1 h−1). [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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