View in EDS

Direct electrochemistry and electrocatalysis of hemoglobin at three-dimensional gold film electrode modified with self-assembled monolayers of 3-mercaptopropylphosphonic acid

Saved in:
Bibliographic Details
Title: Direct electrochemistry and electrocatalysis of hemoglobin at three-dimensional gold film electrode modified with self-assembled monolayers of 3-mercaptopropylphosphonic acid
Authors: Chen, Yu1, Yang, Xiao-Jing1, Guo, Li-Rong1, Li, Jing2, Xia, Xing-Hua2 xhxia@nju.edu.cn, Zheng, Li-Min1 lmzheng@nju.edu.cn
Source: Analytica Chimica Acta. Jun2009, Vol. 644 Issue 1/2, p83-89. 7p.
Subjects: Electrocatalysis, Electrochemistry, Hemoglobins, Gold compounds, Metallic films, Electrodes, Molecular self-assembly, Phosphonic acids
Abstract: Abstract: Multilayered hemoglobin (Hb) molecules were successfully immobilized on three-dimensional gold film electrode modified with self-assembled monolayers (SAMs) of 3-mercaptopropylphosphonic acid. Direct electrochemistry of the immobilized multilayered Hb occurs with high thermal stability and electrochemical stability. In the multilayered Hb film, the most inner Hb molecules can directly transfer electron with the electrode, while the Hb protein beyond this layer communicates electron with the electrode via protein–protein electron exchange. In addition, the proposed functional interface can greatly enhance electron transfer rate of the immobilized Hb protein (k s =15.8±2.0s−1) due to the increase of roughness of the gold substrate. Under optimized experimental conditions, the multilayered Hb film displays good bioelectrocatalytic activity toward the reduction of hydrogen peroxide. This electrochemical sensor shows fast response (less than 1s), wide linear range (7.8×10−8 to 9.1×10−5 M) and low detection limit (2.5×10−8 M), which can be attributed to good mass transport, large Hb proteins loading per unit area and fast electron transfer rate of Hb protein. [Copyright &y& Elsevier]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 40116098
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Direct electrochemistry and electrocatalysis of hemoglobin at three-dimensional gold film electrode modified with self-assembled monolayers of 3-mercaptopropylphosphonic acid
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Chen%2C+Yu%22">Chen, Yu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Xiao-Jing%22">Yang, Xiao-Jing</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guo%2C+Li-Rong%22">Guo, Li-Rong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Jing%22">Li, Jing</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xia%2C+Xing-Hua%22">Xia, Xing-Hua</searchLink><relatesTo>2</relatesTo><i> xhxia@nju.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zheng%2C+Li-Min%22">Zheng, Li-Min</searchLink><relatesTo>1</relatesTo><i> lmzheng@nju.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Jun2009, Vol. 644 Issue 1/2, p83-89. 7p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Electrocatalysis%22">Electrocatalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Electrochemistry%22">Electrochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Hemoglobins%22">Hemoglobins</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+compounds%22">Gold compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Metallic+films%22">Metallic films</searchLink><br /><searchLink fieldCode="DE" term="%22Electrodes%22">Electrodes</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+self-assembly%22">Molecular self-assembly</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphonic+acids%22">Phosphonic acids</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Multilayered hemoglobin (Hb) molecules were successfully immobilized on three-dimensional gold film electrode modified with self-assembled monolayers (SAMs) of 3-mercaptopropylphosphonic acid. Direct electrochemistry of the immobilized multilayered Hb occurs with high thermal stability and electrochemical stability. In the multilayered Hb film, the most inner Hb molecules can directly transfer electron with the electrode, while the Hb protein beyond this layer communicates electron with the electrode via protein–protein electron exchange. In addition, the proposed functional interface can greatly enhance electron transfer rate of the immobilized Hb protein (k s =15.8±2.0s−1) due to the increase of roughness of the gold substrate. Under optimized experimental conditions, the multilayered Hb film displays good bioelectrocatalytic activity toward the reduction of hydrogen peroxide. This electrochemical sensor shows fast response (less than 1s), wide linear range (7.8×10−8 to 9.1×10−5 M) and low detection limit (2.5×10−8 M), which can be attributed to good mass transport, large Hb proteins loading per unit area and fast electron transfer rate of Hb protein. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=40116098
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.aca.2009.04.027
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 83
    Subjects:
      – SubjectFull: Electrocatalysis
        Type: general
      – SubjectFull: Electrochemistry
        Type: general
      – SubjectFull: Hemoglobins
        Type: general
      – SubjectFull: Gold compounds
        Type: general
      – SubjectFull: Metallic films
        Type: general
      – SubjectFull: Electrodes
        Type: general
      – SubjectFull: Molecular self-assembly
        Type: general
      – SubjectFull: Phosphonic acids
        Type: general
    Titles:
      – TitleFull: Direct electrochemistry and electrocatalysis of hemoglobin at three-dimensional gold film electrode modified with self-assembled monolayers of 3-mercaptopropylphosphonic acid
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Chen, Yu
      – PersonEntity:
          Name:
            NameFull: Yang, Xiao-Jing
      – PersonEntity:
          Name:
            NameFull: Guo, Li-Rong
      – PersonEntity:
          Name:
            NameFull: Li, Jing
      – PersonEntity:
          Name:
            NameFull: Xia, Xing-Hua
      – PersonEntity:
          Name:
            NameFull: Zheng, Li-Min
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 30
              M: 06
              Text: Jun2009
              Type: published
              Y: 2009
          Identifiers:
            – Type: issn-print
              Value: 00032670
          Numbering:
            – Type: volume
              Value: 644
            – Type: issue
              Value: 1/2
          Titles:
            – TitleFull: Analytica Chimica Acta
              Type: main
ResultId 1