Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea.
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| Title: | Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea. |
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| Authors: | Nagaoka, T.1, Doullah, M. A. U.2, Matsumoto, S.3, Kawasaki, S.4, Ishikawa, T.1, Hori, H.1, Okazaki, K.1,5 okazaki@agr.niigata-u.ac.jp |
| Source: | Theoretical & Applied Genetics. May2010, Vol. 120 Issue 7, p1335-1346. 12p. 2 Diagrams, 2 Charts, 2 Graphs. |
| Subjects: | Cole crops, Biomarkers, Plant genetics, Heredity, Genes, Glucosinolates |
| Abstract: | To perform comparative studies of CR (clubroot resistance) loci in Brassica oleracea and Brassica rapa and to develop marker-assisted selection in B. oleracea, we constructed a B. oleracea map, including specific markers linked to CR genes of B. rapa. We also analyzed CR-QTLs using the mean phenotypes of F3 progenies from the cross of a resistant double-haploid line (Anju) with a susceptible double-haploid line (GC). In the nine linkage groups obtained (O1-O9), the major QTL, pb-Bo(Anju)1, was derived from Anju with a maximum LOD score (13.7) in O2. The QTL (LOD 5.1) located in O5, pb-Bo(GC)1, was derived from the susceptible GC. Other QTLs with smaller effects were found in O2, O3, and O7. Based on common markers, it was possible to compare our finding CR-QTLs with the B. oleracea CR loci reported by previous authors; pb-Bo(GC)1 may be identical to the CR-QTL reported previously or a different member contained in the same CR gene cluster. In total, the markers linked to seven B. rapa CR genes were mapped on the B. oleracea map. Based on the mapping position and markers of the CR genes, informative comparative studies of CR loci between B. oleracea and B. rapa were performed. Our map discloses specific primer sequences linked to CR genes and includes public SSR markers that will promote pyramiding CR genes in intra- and inter-specific crosses in Brassica crops. Five genes involved in glucosinolates biosynthesis were also mapped, and GSL-BoELONG and GSL-BoPro were found to be linked to the pb-Bo(Anju)1 and Bo(GC)1 loci, respectively. The linkage drag associated with the CR-QTLs is briefly discussed. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 49133193 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nagaoka%2C+T%2E%22">Nagaoka, T.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Doullah%2C+M%2E+A%2E+U%2E%22">Doullah, M. A. U.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Matsumoto%2C+S%2E%22">Matsumoto, S.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Kawasaki%2C+S%2E%22">Kawasaki, S.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Ishikawa%2C+T%2E%22">Ishikawa, T.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hori%2C+H%2E%22">Hori, H.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Okazaki%2C+K%2E%22">Okazaki, K.</searchLink><relatesTo>1,5</relatesTo><i> okazaki@agr.niigata-u.ac.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. May2010, Vol. 120 Issue 7, p1335-1346. 12p. 2 Diagrams, 2 Charts, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cole+crops%22">Cole crops</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetics%22">Plant genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Heredity%22">Heredity</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Glucosinolates%22">Glucosinolates</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To perform comparative studies of CR (clubroot resistance) loci in Brassica oleracea and Brassica rapa and to develop marker-assisted selection in B. oleracea, we constructed a B. oleracea map, including specific markers linked to CR genes of B. rapa. We also analyzed CR-QTLs using the mean phenotypes of F3 progenies from the cross of a resistant double-haploid line (Anju) with a susceptible double-haploid line (GC). In the nine linkage groups obtained (O1-O9), the major QTL, pb-Bo(Anju)1, was derived from Anju with a maximum LOD score (13.7) in O2. The QTL (LOD 5.1) located in O5, pb-Bo(GC)1, was derived from the susceptible GC. Other QTLs with smaller effects were found in O2, O3, and O7. Based on common markers, it was possible to compare our finding CR-QTLs with the B. oleracea CR loci reported by previous authors; pb-Bo(GC)1 may be identical to the CR-QTL reported previously or a different member contained in the same CR gene cluster. In total, the markers linked to seven B. rapa CR genes were mapped on the B. oleracea map. Based on the mapping position and markers of the CR genes, informative comparative studies of CR loci between B. oleracea and B. rapa were performed. Our map discloses specific primer sequences linked to CR genes and includes public SSR markers that will promote pyramiding CR genes in intra- and inter-specific crosses in Brassica crops. Five genes involved in glucosinolates biosynthesis were also mapped, and GSL-BoELONG and GSL-BoPro were found to be linked to the pb-Bo(Anju)1 and Bo(GC)1 loci, respectively. The linkage drag associated with the CR-QTLs is briefly discussed. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-010-1259-z Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1335 Subjects: – SubjectFull: Cole crops Type: general – SubjectFull: Biomarkers Type: general – SubjectFull: Plant genetics Type: general – SubjectFull: Heredity Type: general – SubjectFull: Genes Type: general – SubjectFull: Glucosinolates Type: general Titles: – TitleFull: Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nagaoka, T. – PersonEntity: Name: NameFull: Doullah, M. A. U. – PersonEntity: Name: NameFull: Matsumoto, S. – PersonEntity: Name: NameFull: Kawasaki, S. – PersonEntity: Name: NameFull: Ishikawa, T. – PersonEntity: Name: NameFull: Hori, H. – PersonEntity: Name: NameFull: Okazaki, K. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 120 – Type: issue Value: 7 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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