Dynamics of Pseudomonas aeruginosa association with anionic hydrogel surfaces in the presence of aqueous divalent-cation salts
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| Title: | Dynamics of Pseudomonas aeruginosa association with anionic hydrogel surfaces in the presence of aqueous divalent-cation salts |
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| Authors: | Tran, Victoria B.1, Sung, Ye Suel1, Fleiszig, Suzanne M.J.2,3,4, Evans, David J.2,5, Radke, C.J.1,3 radke@berkeley.edu |
| Source: | Journal of Colloid & Interface Science. Oct2011, Vol. 362 Issue 1, p58-66. 9p. |
| Subjects: | Pseudomonas aeruginosa, Surface chemistry, Cations, Methyl methacrylate, Acrylic acid, Soft contact lenses, Hydrogels, Chemical kinetics, Phase-contrast microscopy |
| Abstract: | Abstract: Binding of bacteria to solid surfaces is complex with many aspects incompletely understood. We investigate Pseudomonas aeruginosa uptake kinetics onto hydrogel surfaces representative of soft-contact lenses made of nonionic poly(2-hydroxyethylmethacrylate) (p-HEMA), anionic poly(methacrylic acid) (p-MAA), and anionic poly(acrylic acid) (p-AA). Using a parallel-plate flow cell under phase-contrast microscopy, we document a kinetic “burst” at the anionic hydrogel surface: dilute aqueous P. aeruginosa first rapidly accumulates and then rapidly depletes. Upon continuing flow, divalent cations in the suspending solution sorb into the hydrogel network causing the previously surface-accumulated bacteria to desorb. The number of bacteria eventually bound to the surface is low compared to the nonionic p-HEMA hydrogel. We propose that the kinetic burst is due to reversible divalent-cation bridging between the anionic bacteria and the negatively charged hydrogel surface. The number of surface bridging sites diminishes as divalent cations impregnate into and collapse the gel. P. aeruginosa association with the surface then falls. Low eventual binding of P. aeruginosa to the anionic hydrogel is ascribed to increased surface hydrophilicity compared to the counterpart nonionic p-HEMA hydrogel. [Copyright &y& Elsevier] |
| Copyright of Journal of Colloid & Interface Science is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 63555015 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Dynamics of Pseudomonas aeruginosa association with anionic hydrogel surfaces in the presence of aqueous divalent-cation salts – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tran%2C+Victoria+B%2E%22">Tran, Victoria B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sung%2C+Ye+Suel%22">Sung, Ye Suel</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fleiszig%2C+Suzanne+M%2EJ%2E%22">Fleiszig, Suzanne M.J.</searchLink><relatesTo>2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Evans%2C+David+J%2E%22">Evans, David J.</searchLink><relatesTo>2,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Radke%2C+C%2EJ%2E%22">Radke, C.J.</searchLink><relatesTo>1,3</relatesTo><i> radke@berkeley.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Colloid+%26+Interface+Science%22">Journal of Colloid & Interface Science</searchLink>. Oct2011, Vol. 362 Issue 1, p58-66. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Pseudomonas+aeruginosa%22">Pseudomonas aeruginosa</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+chemistry%22">Surface chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Cations%22">Cations</searchLink><br /><searchLink fieldCode="DE" term="%22Methyl+methacrylate%22">Methyl methacrylate</searchLink><br /><searchLink fieldCode="DE" term="%22Acrylic+acid%22">Acrylic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Soft+contact+lenses%22">Soft contact lenses</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogels%22">Hydrogels</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+kinetics%22">Chemical kinetics</searchLink><br /><searchLink fieldCode="DE" term="%22Phase-contrast+microscopy%22">Phase-contrast microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: Binding of bacteria to solid surfaces is complex with many aspects incompletely understood. We investigate Pseudomonas aeruginosa uptake kinetics onto hydrogel surfaces representative of soft-contact lenses made of nonionic poly(2-hydroxyethylmethacrylate) (p-HEMA), anionic poly(methacrylic acid) (p-MAA), and anionic poly(acrylic acid) (p-AA). Using a parallel-plate flow cell under phase-contrast microscopy, we document a kinetic “burst” at the anionic hydrogel surface: dilute aqueous P. aeruginosa first rapidly accumulates and then rapidly depletes. Upon continuing flow, divalent cations in the suspending solution sorb into the hydrogel network causing the previously surface-accumulated bacteria to desorb. The number of bacteria eventually bound to the surface is low compared to the nonionic p-HEMA hydrogel. We propose that the kinetic burst is due to reversible divalent-cation bridging between the anionic bacteria and the negatively charged hydrogel surface. The number of surface bridging sites diminishes as divalent cations impregnate into and collapse the gel. P. aeruginosa association with the surface then falls. Low eventual binding of P. aeruginosa to the anionic hydrogel is ascribed to increased surface hydrophilicity compared to the counterpart nonionic p-HEMA hydrogel. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Colloid & Interface Science is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jcis.2011.06.012 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 58 Subjects: – SubjectFull: Pseudomonas aeruginosa Type: general – SubjectFull: Surface chemistry Type: general – SubjectFull: Cations Type: general – SubjectFull: Methyl methacrylate Type: general – SubjectFull: Acrylic acid Type: general – SubjectFull: Soft contact lenses Type: general – SubjectFull: Hydrogels Type: general – SubjectFull: Chemical kinetics Type: general – SubjectFull: Phase-contrast microscopy Type: general Titles: – TitleFull: Dynamics of Pseudomonas aeruginosa association with anionic hydrogel surfaces in the presence of aqueous divalent-cation salts Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tran, Victoria B. – PersonEntity: Name: NameFull: Sung, Ye Suel – PersonEntity: Name: NameFull: Fleiszig, Suzanne M.J. – PersonEntity: Name: NameFull: Evans, David J. – PersonEntity: Name: NameFull: Radke, C.J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 00219797 Numbering: – Type: volume Value: 362 – Type: issue Value: 1 Titles: – TitleFull: Journal of Colloid & Interface Science Type: main |
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