Rhizobial position as a main determinant in the problem of competition for nodulation in soybean.
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| Title: | Rhizobial position as a main determinant in the problem of competition for nodulation in soybean. |
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| Authors: | López-García, Silvina L, Vázquez, Tirso E. E1, Favelukes, Gabriel1, Lodeiro, Aníbal R1 |
| Source: | Environmental Microbiology. Apr2002, Vol. 4 Issue 4, p216-224. 9p. |
| Subjects: | Competition (Biology), Microbial inoculants, Rhizobium |
| Abstract: | Summary Selected Bradyrhizobium japonicum strains inoculated on soybean seeds often fail to occupy a significant proportion of nodules when a competitor rhizobial population is established in the soil. This competition problem could result from a genetic/ physiological advantage of the adapted soil population over the introduced inoculant or from a positional advantage, as the soil population already occupies the soil profile where the roots will penetrate, whereas the inoculant remains concentrated around the seeds. Here, we have assessed the contribution of these factors with a laboratory model in which a rhizobial population is established in sterile vermiculite. We observed that the wild-type strain B. japonicum LP 3004 was able to grow in pots with N-free plant nutrient solution-watered vermiculite for six or seven generations with a duplication rate of at least 0.7 day- 1 . In addition, the rhizobial population persisted for 3 months with 106 –107 colony-forming units ml- 1 of the vermiculite-retained solution. N-starved, young rhizobial cultures are more efficient in performing several steps along their early association with soybean roots. However, N starvation during growth of rhizobia used for seed inoculation did not enhance their competitiveness against a 1 month vermiculite-established rhizobial population, which occupied more than 72% of the nodules. When a similarly established rhizobial population was recovered from the vermiculite and homogeneously suspended in plant nutrient solution, these cells were significantly less competitive (29% of nodules occupied) than rhizobia obtained from a fresh, logarithmic culture in a N-poor minimal medium, thus indicating that cell position rather than intrinsic competitiveness was the determinant for nodule occupation. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 6652655 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Rhizobial position as a main determinant in the problem of competition for nodulation in soybean. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22López-García%2C+Silvina+L%22">López-García, Silvina L</searchLink><br /><searchLink fieldCode="AR" term="%22Vázquez%2C+Tirso+E%2E+E%22">Vázquez, Tirso E. E</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Favelukes%2C+Gabriel%22">Favelukes, Gabriel</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lodeiro%2C+Aníbal+R%22">Lodeiro, Aníbal R</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Apr2002, Vol. 4 Issue 4, p216-224. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Competition+%28Biology%29%22">Competition (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+inoculants%22">Microbial inoculants</searchLink><br /><searchLink fieldCode="DE" term="%22Rhizobium%22">Rhizobium</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Summary Selected Bradyrhizobium japonicum strains inoculated on soybean seeds often fail to occupy a significant proportion of nodules when a competitor rhizobial population is established in the soil. This competition problem could result from a genetic/ physiological advantage of the adapted soil population over the introduced inoculant or from a positional advantage, as the soil population already occupies the soil profile where the roots will penetrate, whereas the inoculant remains concentrated around the seeds. Here, we have assessed the contribution of these factors with a laboratory model in which a rhizobial population is established in sterile vermiculite. We observed that the wild-type strain B. japonicum LP 3004 was able to grow in pots with N-free plant nutrient solution-watered vermiculite for six or seven generations with a duplication rate of at least 0.7 day- 1 . In addition, the rhizobial population persisted for 3 months with 106 –107 colony-forming units ml- 1 of the vermiculite-retained solution. N-starved, young rhizobial cultures are more efficient in performing several steps along their early association with soybean roots. However, N starvation during growth of rhizobia used for seed inoculation did not enhance their competitiveness against a 1 month vermiculite-established rhizobial population, which occupied more than 72% of the nodules. When a similarly established rhizobial population was recovered from the vermiculite and homogeneously suspended in plant nutrient solution, these cells were significantly less competitive (29% of nodules occupied) than rhizobia obtained from a fresh, logarithmic culture in a N-poor minimal medium, thus indicating that cell position rather than intrinsic competitiveness was the determinant for nodule occupation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1462-2920.2002.00287.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 216 Subjects: – SubjectFull: Competition (Biology) Type: general – SubjectFull: Microbial inoculants Type: general – SubjectFull: Rhizobium Type: general Titles: – TitleFull: Rhizobial position as a main determinant in the problem of competition for nodulation in soybean. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: López-García, Silvina L – PersonEntity: Name: NameFull: Vázquez, Tirso E. E – PersonEntity: Name: NameFull: Favelukes, Gabriel – PersonEntity: Name: NameFull: Lodeiro, Aníbal R IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 14622912 Numbering: – Type: volume Value: 4 – Type: issue Value: 4 Titles: – TitleFull: Environmental Microbiology Type: main |
| ResultId | 1 |
