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Nucleotide excision repair by extracts of human fetal hepatocytes

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Title: Nucleotide excision repair by extracts of human fetal hepatocytes
Authors: Capovilla, Alexio1, Arbuthnot, Patrick 126arbu@chiron.wits.ac.za
Source: FEBS Letters. May2002, Vol. 518 Issue 1-3, p144. 5p.
Subjects: Nucleotides, Liver cells
Abstract: Human hepatocytes are particularly exposed to genotoxins, and nucleotide excision repair (NER) in these cells is essential for the maintenance of genome integrity. To characterize NER under conditions that closely resemble the pathway in vivo, we report the preparation and use of primary human fetal liver extracts to define the repair of a 1,3-intrastrand d(GpTpG)-cisplatin DNA lesion. Endonucleolytic cleavage at unique sites on either side of the adduct occurs at similar positions to the dominant NER incisions that have been reported for HeLa extracts. However, incisions effected by primary hepatocyte extracts are more precise as no secondary cleavage sites are detected 5′ and 3′ to the cisplatin lesion. [Copyright &y& Elsevier]
Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 7801993
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Nucleotide excision repair by extracts of human fetal hepatocytes
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  Data: <searchLink fieldCode="AR" term="%22Capovilla%2C+Alexio%22">Capovilla, Alexio</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Arbuthnot%2C+Patrick%22">Arbuthnot, Patrick</searchLink><i> 126arbu@chiron.wits.ac.za</i>
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  Data: <searchLink fieldCode="JN" term="%22FEBS+Letters%22">FEBS Letters</searchLink>. May2002, Vol. 518 Issue 1-3, p144. 5p.
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  Data: Human hepatocytes are particularly exposed to genotoxins, and nucleotide excision repair (NER) in these cells is essential for the maintenance of genome integrity. To characterize NER under conditions that closely resemble the pathway in vivo, we report the preparation and use of primary human fetal liver extracts to define the repair of a 1,3-intrastrand d(GpTpG)-cisplatin DNA lesion. Endonucleolytic cleavage at unique sites on either side of the adduct occurs at similar positions to the dominant NER incisions that have been reported for HeLa extracts. However, incisions effected by primary hepatocyte extracts are more precise as no secondary cleavage sites are detected 5′ and 3′ to the cisplatin lesion. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/S0014-5793(02)02686-8
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      – Code: eng
        Text: English
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        PageCount: 5
        StartPage: 144
    Subjects:
      – SubjectFull: Nucleotides
        Type: general
      – SubjectFull: Liver cells
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      – TitleFull: Nucleotide excision repair by extracts of human fetal hepatocytes
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            NameFull: Capovilla, Alexio
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              Text: May2002
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