Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles
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| Title: | Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles |
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| Authors: | Tang, Lin1,2 tanglin@hnu.edu.cn, Lei, Xiaoxia1,2, Zeng, Guangming1,2 zgming@hnu.edu.cn, Liu, Yuanyuan1,2, Peng, Yanrong1,2, Wu, Mengshi1,2, Zhang, Yi1,2, Liu, Can1,2, Li, Zhen1,2, Shen, Guoli3 |
| Source: | Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Dec2012, Vol. 99, p390-393. 4p. |
| Subjects: | NAD (Coenzyme), Gold nanoparticles, Silver nanoparticles, Biological assay, Nicotinamide, Catalysts, Ultraviolet radiation |
| Abstract: | Abstract: We have developed an optical assay for NADH (Dihydronicotinamide adenine dinucleotide) based on the catalytic growth of gold–silver core–shell nanoparticles (Au–Ag–CSNPs). The nanoparticles were immobilized on pretreated glass slide and are shown to catalyze the NADH-mediated reduction of Ag(I) ions in the presence of 1,4-benzoquinone and cetyltrimethyl ammonium ion. This leads to the formation of Au–Ag–CSNPs on the glass. The absorption peak of the Au–Ag–CSNPs at 415nm increases with the concentration of NADH in the solution used, and this can be measured by UV–vis photometry. High-resolution scanning electron microscopy analysis of the morphology of the surface of the Au–Ag–CSNPs before and after the catalytic reaction revealed a growth of their diameter. Under optimal conditions, NADH can be determined in the concentration range from 0.2 to 3.2mM, and the detection limit is 15.6μM. The sensor has good precision and good storage stability, simple in operation, and can be fabricated at low costs, which made it suitable for the determination of NADH in complex biological systems and in related degradation processes of contaminants. [Copyright &y& Elsevier] |
| Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 83190480 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tang%2C+Lin%22">Tang, Lin</searchLink><relatesTo>1,2</relatesTo><i> tanglin@hnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Lei%2C+Xiaoxia%22">Lei, Xiaoxia</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zeng%2C+Guangming%22">Zeng, Guangming</searchLink><relatesTo>1,2</relatesTo><i> zgming@hnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yuanyuan%22">Liu, Yuanyuan</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Peng%2C+Yanrong%22">Peng, Yanrong</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Mengshi%22">Wu, Mengshi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yi%22">Zhang, Yi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Can%22">Liu, Can</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Zhen%22">Li, Zhen</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Shen%2C+Guoli%22">Shen, Guoli</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Dec2012, Vol. 99, p390-393. 4p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22NAD+%28Coenzyme%29%22">NAD (Coenzyme)</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Silver+nanoparticles%22">Silver nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Nicotinamide%22">Nicotinamide</searchLink><br /><searchLink fieldCode="DE" term="%22Catalysts%22">Catalysts</searchLink><br /><searchLink fieldCode="DE" term="%22Ultraviolet+radiation%22">Ultraviolet radiation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: We have developed an optical assay for NADH (Dihydronicotinamide adenine dinucleotide) based on the catalytic growth of gold–silver core–shell nanoparticles (Au–Ag–CSNPs). The nanoparticles were immobilized on pretreated glass slide and are shown to catalyze the NADH-mediated reduction of Ag(I) ions in the presence of 1,4-benzoquinone and cetyltrimethyl ammonium ion. This leads to the formation of Au–Ag–CSNPs on the glass. The absorption peak of the Au–Ag–CSNPs at 415nm increases with the concentration of NADH in the solution used, and this can be measured by UV–vis photometry. High-resolution scanning electron microscopy analysis of the morphology of the surface of the Au–Ag–CSNPs before and after the catalytic reaction revealed a growth of their diameter. Under optimal conditions, NADH can be determined in the concentration range from 0.2 to 3.2mM, and the detection limit is 15.6μM. The sensor has good precision and good storage stability, simple in operation, and can be fabricated at low costs, which made it suitable for the determination of NADH in complex biological systems and in related degradation processes of contaminants. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.saa.2012.09.011 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 390 Subjects: – SubjectFull: NAD (Coenzyme) Type: general – SubjectFull: Gold nanoparticles Type: general – SubjectFull: Silver nanoparticles Type: general – SubjectFull: Biological assay Type: general – SubjectFull: Nicotinamide Type: general – SubjectFull: Catalysts Type: general – SubjectFull: Ultraviolet radiation Type: general Titles: – TitleFull: Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tang, Lin – PersonEntity: Name: NameFull: Lei, Xiaoxia – PersonEntity: Name: NameFull: Zeng, Guangming – PersonEntity: Name: NameFull: Liu, Yuanyuan – PersonEntity: Name: NameFull: Peng, Yanrong – PersonEntity: Name: NameFull: Wu, Mengshi – PersonEntity: Name: NameFull: Zhang, Yi – PersonEntity: Name: NameFull: Liu, Can – PersonEntity: Name: NameFull: Li, Zhen – PersonEntity: Name: NameFull: Shen, Guoli IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 12 Text: Dec2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 13861425 Numbering: – Type: volume Value: 99 Titles: – TitleFull: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy Type: main |
| ResultId | 1 |