Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles

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Title: Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles
Authors: Tang, Lin1,2 tanglin@hnu.edu.cn, Lei, Xiaoxia1,2, Zeng, Guangming1,2 zgming@hnu.edu.cn, Liu, Yuanyuan1,2, Peng, Yanrong1,2, Wu, Mengshi1,2, Zhang, Yi1,2, Liu, Can1,2, Li, Zhen1,2, Shen, Guoli3
Source: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Dec2012, Vol. 99, p390-393. 4p.
Subjects: NAD (Coenzyme), Gold nanoparticles, Silver nanoparticles, Biological assay, Nicotinamide, Catalysts, Ultraviolet radiation
Abstract: Abstract: We have developed an optical assay for NADH (Dihydronicotinamide adenine dinucleotide) based on the catalytic growth of gold–silver core–shell nanoparticles (Au–Ag–CSNPs). The nanoparticles were immobilized on pretreated glass slide and are shown to catalyze the NADH-mediated reduction of Ag(I) ions in the presence of 1,4-benzoquinone and cetyltrimethyl ammonium ion. This leads to the formation of Au–Ag–CSNPs on the glass. The absorption peak of the Au–Ag–CSNPs at 415nm increases with the concentration of NADH in the solution used, and this can be measured by UV–vis photometry. High-resolution scanning electron microscopy analysis of the morphology of the surface of the Au–Ag–CSNPs before and after the catalytic reaction revealed a growth of their diameter. Under optimal conditions, NADH can be determined in the concentration range from 0.2 to 3.2mM, and the detection limit is 15.6μM. The sensor has good precision and good storage stability, simple in operation, and can be fabricated at low costs, which made it suitable for the determination of NADH in complex biological systems and in related degradation processes of contaminants. [Copyright &y& Elsevier]
Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Group: Ti
  Data: Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles
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  Data: <searchLink fieldCode="AR" term="%22Tang%2C+Lin%22">Tang, Lin</searchLink><relatesTo>1,2</relatesTo><i> tanglin@hnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Lei%2C+Xiaoxia%22">Lei, Xiaoxia</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zeng%2C+Guangming%22">Zeng, Guangming</searchLink><relatesTo>1,2</relatesTo><i> zgming@hnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yuanyuan%22">Liu, Yuanyuan</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Peng%2C+Yanrong%22">Peng, Yanrong</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Mengshi%22">Wu, Mengshi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yi%22">Zhang, Yi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Can%22">Liu, Can</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Zhen%22">Li, Zhen</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Shen%2C+Guoli%22">Shen, Guoli</searchLink><relatesTo>3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Dec2012, Vol. 99, p390-393. 4p.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22NAD+%28Coenzyme%29%22">NAD (Coenzyme)</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Silver+nanoparticles%22">Silver nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Nicotinamide%22">Nicotinamide</searchLink><br /><searchLink fieldCode="DE" term="%22Catalysts%22">Catalysts</searchLink><br /><searchLink fieldCode="DE" term="%22Ultraviolet+radiation%22">Ultraviolet radiation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: We have developed an optical assay for NADH (Dihydronicotinamide adenine dinucleotide) based on the catalytic growth of gold–silver core–shell nanoparticles (Au–Ag–CSNPs). The nanoparticles were immobilized on pretreated glass slide and are shown to catalyze the NADH-mediated reduction of Ag(I) ions in the presence of 1,4-benzoquinone and cetyltrimethyl ammonium ion. This leads to the formation of Au–Ag–CSNPs on the glass. The absorption peak of the Au–Ag–CSNPs at 415nm increases with the concentration of NADH in the solution used, and this can be measured by UV–vis photometry. High-resolution scanning electron microscopy analysis of the morphology of the surface of the Au–Ag–CSNPs before and after the catalytic reaction revealed a growth of their diameter. Under optimal conditions, NADH can be determined in the concentration range from 0.2 to 3.2mM, and the detection limit is 15.6μM. The sensor has good precision and good storage stability, simple in operation, and can be fabricated at low costs, which made it suitable for the determination of NADH in complex biological systems and in related degradation processes of contaminants. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.saa.2012.09.011
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      – Code: eng
        Text: English
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        PageCount: 4
        StartPage: 390
    Subjects:
      – SubjectFull: NAD (Coenzyme)
        Type: general
      – SubjectFull: Gold nanoparticles
        Type: general
      – SubjectFull: Silver nanoparticles
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      – SubjectFull: Nicotinamide
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      – SubjectFull: Catalysts
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      – SubjectFull: Ultraviolet radiation
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      – TitleFull: Optical detection of NADH based on biocatalytic growth of Au–Ag core–shell nanoparticles
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              M: 12
              Text: Dec2012
              Type: published
              Y: 2012
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