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Determination of trenbolone and its metabolite in bovine fluids by liquid chromatography–tandem mass spectrometry

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Title: Determination of trenbolone and its metabolite in bovine fluids by liquid chromatography–tandem mass spectrometry
Authors: Buiarelli, F., Cartoni, G.P. giampaolo.cartoni@uniroma1.it, Coccioli, F., De Rossi, A., Neri, B.
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Jan2003, Vol. 784 Issue 1, p1. 15p.
Subjects: Metabolites, Cattle, Liquid chromatography, Mass spectrometry, Urinalysis
Abstract: A liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method has been developed for the determination of trenbolone in bovine urine and serum. The aim was a control of the misuse of trenbolone in food-producing animals. The procedure involved, in both cases, a preliminary solid-phase clean-up followed by a liquid–liquid extraction for urine samples after a preliminary enzymatic hydrolysis. The extracts have been directly analysed by reversed-phase LC–MS–MS in selected reaction monitoring (SRM), acquiring two diagnostic product ions from the chosen precursor [M+H]+. The procedures were validated across the concentration range of 1–1500 ng/ml. The linearity, the inter- and intra-day accuracy and precision have been determined. The procedure was specific and the accuracy values were better than 20% at the limit of quantitation of spiked samples. The limit of quantification (LOQ) and the limit of detection (LOD) were, respectively, 1 ng/ml and 350 pg/ml for urine and serum. According to the draft, SANCO/1805/2000, we determined the decision limit CCα and the detection capability CCβ. The recovery values for urine ranged from 87 to 128%, and for plasma the recovery was 70±4%. The procedure proved to be simple and suitable for routine and confirmatory purposes such as those developed for residue studies. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 8722129
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Determination of trenbolone and its metabolite in bovine fluids by liquid chromatography–tandem mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Buiarelli%2C+F%2E%22">Buiarelli, F.</searchLink><br /><searchLink fieldCode="AR" term="%22Cartoni%2C+G%2EP%2E%22">Cartoni, G.P.</searchLink><i> giampaolo.cartoni@uniroma1.it</i><br /><searchLink fieldCode="AR" term="%22Coccioli%2C+F%2E%22">Coccioli, F.</searchLink><br /><searchLink fieldCode="AR" term="%22De+Rossi%2C+A%2E%22">De Rossi, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Neri%2C+B%2E%22">Neri, B.</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink><br /><searchLink fieldCode="DE" term="%22Cattle%22">Cattle</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Urinalysis%22">Urinalysis</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: A liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method has been developed for the determination of trenbolone in bovine urine and serum. The aim was a control of the misuse of trenbolone in food-producing animals. The procedure involved, in both cases, a preliminary solid-phase clean-up followed by a liquid–liquid extraction for urine samples after a preliminary enzymatic hydrolysis. The extracts have been directly analysed by reversed-phase LC–MS–MS in selected reaction monitoring (SRM), acquiring two diagnostic product ions from the chosen precursor [M+H]+. The procedures were validated across the concentration range of 1–1500 ng/ml. The linearity, the inter- and intra-day accuracy and precision have been determined. The procedure was specific and the accuracy values were better than 20% at the limit of quantitation of spiked samples. The limit of quantification (LOQ) and the limit of detection (LOD) were, respectively, 1 ng/ml and 350 pg/ml for urine and serum. According to the draft, SANCO/1805/2000, we determined the decision limit CCα and the detection capability CCβ. The recovery values for urine ranged from 87 to 128%, and for plasma the recovery was 70±4%. The procedure proved to be simple and suitable for routine and confirmatory purposes such as those developed for residue studies. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/S1570-0232(02)00435-X
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        Text: English
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      – SubjectFull: Liquid chromatography
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      – SubjectFull: Mass spectrometry
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      – SubjectFull: Urinalysis
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            NameFull: Coccioli, F.
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              Text: Jan2003
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