Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors.
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| Title: | Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors. |
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| Authors: | Freeman, Michael H.1, Hall, Jackson R.1, Leopold, Michael C.1 mleopold@richmond.edu |
| Source: | Analytical Chemistry. 4/16/2013, Vol. 85 Issue 8, p4057-4065. 9p. |
| Subjects: | Xerogels, Gold nanoparticles, Amperometric sensors, Glucose analysis, Biosensors, Platinum electrodes, Glucose oxidase |
| Abstract: | First-generation amperometric glucose biosensors incorporating alkanethiolate-protected gold nanoparticles, monolayer protected clusters (MPCs), within a xerogel matrix are investigated as model systems for nanomaterial-assisted electrochemical sensing strategies. The xerogel biosensors are comprised of platinum electrodes modified with composite films of (3-mercaptopropyl)trimethoxy silane xerogel embedded with glucose oxidase enzyme, doped with Au225(C6)75 MPCs, and coated with an outer polyurethane layer. Electrochemistry and scanning/transmission electron microscopy, including cross-sectional TEM, show sensor construction, humidity effects on xerogel structure, and successful incorporation of MPCs. Analytical performance of the biosensor scheme with and without MPC doping of the xerogel is determined from direct glucose injection during amperometry. MPC-doped xerogels yield significant enhancement of several sensor attributes compared to analogous films without nanoparticles: doubling of the linear range, sensitivity enhancement by an order of magnitude, and 4-fold faster response times accompany long-term stability and resistance to common interfering agents that are competitive with current glucose biosensing literature. Ligand chain length and the MPC/silane ratio studies suggest the MPC-induced enhancements are critically related to structure-function relationships, particularly those affecting interparticle electronic communication where the MPC network behaves as a three-dimensional extension of the working electrode into the xerogel film, reducing the system's dependence on diffusion and maximizing efficiency of the sensing mechanism. The integration of MPCs as a functional component of amperometric biosensor schemes has implications for future development of biosensors targeting clinically relevant species. [ABSTRACT FROM AUTHOR] |
| Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Freeman%2C+Michael+H%2E%22">Freeman, Michael H.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hall%2C+Jackson+R%2E%22">Hall, Jackson R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Leopold%2C+Michael+C%2E%22">Leopold, Michael C.</searchLink><relatesTo>1</relatesTo><i> mleopold@richmond.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Analytical+Chemistry%22">Analytical Chemistry</searchLink>. 4/16/2013, Vol. 85 Issue 8, p4057-4065. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Xerogels%22">Xerogels</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Amperometric+sensors%22">Amperometric sensors</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose+analysis%22">Glucose analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Platinum+electrodes%22">Platinum electrodes</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose+oxidase%22">Glucose oxidase</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: First-generation amperometric glucose biosensors incorporating alkanethiolate-protected gold nanoparticles, monolayer protected clusters (MPCs), within a xerogel matrix are investigated as model systems for nanomaterial-assisted electrochemical sensing strategies. The xerogel biosensors are comprised of platinum electrodes modified with composite films of (3-mercaptopropyl)trimethoxy silane xerogel embedded with glucose oxidase enzyme, doped with Au225(C6)75 MPCs, and coated with an outer polyurethane layer. Electrochemistry and scanning/transmission electron microscopy, including cross-sectional TEM, show sensor construction, humidity effects on xerogel structure, and successful incorporation of MPCs. Analytical performance of the biosensor scheme with and without MPC doping of the xerogel is determined from direct glucose injection during amperometry. MPC-doped xerogels yield significant enhancement of several sensor attributes compared to analogous films without nanoparticles: doubling of the linear range, sensitivity enhancement by an order of magnitude, and 4-fold faster response times accompany long-term stability and resistance to common interfering agents that are competitive with current glucose biosensing literature. Ligand chain length and the MPC/silane ratio studies suggest the MPC-induced enhancements are critically related to structure-function relationships, particularly those affecting interparticle electronic communication where the MPC network behaves as a three-dimensional extension of the working electrode into the xerogel film, reducing the system's dependence on diffusion and maximizing efficiency of the sensing mechanism. The integration of MPCs as a functional component of amperometric biosensor schemes has implications for future development of biosensors targeting clinically relevant species. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/ac3037188 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 4057 Subjects: – SubjectFull: Xerogels Type: general – SubjectFull: Gold nanoparticles Type: general – SubjectFull: Amperometric sensors Type: general – SubjectFull: Glucose analysis Type: general – SubjectFull: Biosensors Type: general – SubjectFull: Platinum electrodes Type: general – SubjectFull: Glucose oxidase Type: general Titles: – TitleFull: Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Freeman, Michael H. – PersonEntity: Name: NameFull: Hall, Jackson R. – PersonEntity: Name: NameFull: Leopold, Michael C. IsPartOfRelationships: – BibEntity: Dates: – D: 16 M: 04 Text: 4/16/2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 00032700 Numbering: – Type: volume Value: 85 – Type: issue Value: 8 Titles: – TitleFull: Analytical Chemistry Type: main |
| ResultId | 1 |
