Coupling Supported Lipid Bilayer Electrophoresis with Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging.
Saved in:
| Title: | Coupling Supported Lipid Bilayer Electrophoresis with Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging. |
|---|---|
| Authors: | Pace, Hudson P.1, Sherrod, Stacy D.1, Monson, Christopher F.1, Russell, David H.1, Cremer, Paul S.1 psc11@psu.edu |
| Source: | Analytical Chemistry. 6/18/2013, Vol. 85 Issue 12, p6047-6052. 6p. |
| Subjects: | Bilayer lipid membranes, Biomolecules spectra, Biological membranes, Electrophoresis, Matrix-assisted laser desorption-ionization, Mass spectrometry, Gangliosides |
| Abstract: | Herein, we describe a new analytical platform utilizing advances in heterogeneous supported lipid bilayer (SLB) electrophoresis and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging. This platform allowed for the separation and visualization of both charged and neutral lipid membrane components without the need for extrinsic labels. A heterogeneous SLB was created using vesicles containing monosialoganglioside GM1, disialoganglioside GD1b, POPC, as well as the ortho and para isomers of Texas Red-DHPE. These components were then separated electrophoretically into five resolved bands. This represents the most complex separation by SLB electrophoresis performed to date. The SLB samples were flash frozen in liquid ethane and dried under vacuum before imaging with MALDI-MS. Fluorescence microscopy was employed to confirm the position of the Texas Red labeled lipids, which agreed well with the MALDI-MS imaging results. These results clearly demonstrate this platform's ability to isolate and identify nonlabeled membrane components within an SLB. [ABSTRACT FROM AUTHOR] |
| Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 89332826 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Coupling Supported Lipid Bilayer Electrophoresis with Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pace%2C+Hudson+P%2E%22">Pace, Hudson P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sherrod%2C+Stacy+D%2E%22">Sherrod, Stacy D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Monson%2C+Christopher+F%2E%22">Monson, Christopher F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Russell%2C+David+H%2E%22">Russell, David H.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cremer%2C+Paul+S%2E%22">Cremer, Paul S.</searchLink><relatesTo>1</relatesTo><i> psc11@psu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Analytical+Chemistry%22">Analytical Chemistry</searchLink>. 6/18/2013, Vol. 85 Issue 12, p6047-6052. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bilayer+lipid+membranes%22">Bilayer lipid membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Biomolecules+spectra%22">Biomolecules spectra</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+membranes%22">Biological membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophoresis%22">Electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix-assisted+laser+desorption-ionization%22">Matrix-assisted laser desorption-ionization</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Gangliosides%22">Gangliosides</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Herein, we describe a new analytical platform utilizing advances in heterogeneous supported lipid bilayer (SLB) electrophoresis and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging. This platform allowed for the separation and visualization of both charged and neutral lipid membrane components without the need for extrinsic labels. A heterogeneous SLB was created using vesicles containing monosialoganglioside GM1, disialoganglioside GD1b, POPC, as well as the ortho and para isomers of Texas Red-DHPE. These components were then separated electrophoretically into five resolved bands. This represents the most complex separation by SLB electrophoresis performed to date. The SLB samples were flash frozen in liquid ethane and dried under vacuum before imaging with MALDI-MS. Fluorescence microscopy was employed to confirm the position of the Texas Red labeled lipids, which agreed well with the MALDI-MS imaging results. These results clearly demonstrate this platform's ability to isolate and identify nonlabeled membrane components within an SLB. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=89332826 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/ac4008804 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 6047 Subjects: – SubjectFull: Bilayer lipid membranes Type: general – SubjectFull: Biomolecules spectra Type: general – SubjectFull: Biological membranes Type: general – SubjectFull: Electrophoresis Type: general – SubjectFull: Matrix-assisted laser desorption-ionization Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Gangliosides Type: general Titles: – TitleFull: Coupling Supported Lipid Bilayer Electrophoresis with Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pace, Hudson P. – PersonEntity: Name: NameFull: Sherrod, Stacy D. – PersonEntity: Name: NameFull: Monson, Christopher F. – PersonEntity: Name: NameFull: Russell, David H. – PersonEntity: Name: NameFull: Cremer, Paul S. IsPartOfRelationships: – BibEntity: Dates: – D: 18 M: 06 Text: 6/18/2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 00032700 Numbering: – Type: volume Value: 85 – Type: issue Value: 12 Titles: – TitleFull: Analytical Chemistry Type: main |
| ResultId | 1 |
