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Precision and long-term stability of capillary electrophoresis measurement of serum protein zones

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Title: Precision and long-term stability of capillary electrophoresis measurement of serum protein zones
Authors: Luraschi, Paola1, Brambilla, Simona2, Infusino, Ilenia2, Franzini, Carlo2 carlo.franzini@unimi.it
Source: Clinica Chimica Acta. Jul2003, Vol. 333 Issue 1, p41. 5p.
Subjects: Electrophoresis, Blood proteins
Abstract: Background: Analytical evaluations of an available system for capillary zone electrophoresis (CZE) of serum protein have been reported. However, data concerning long-term precision and stability of the system, operated under routine conditions, are lacking. We report data from an internal quality control (QC) scheme, obtained over a 1-year period. Methods: Measurements were done with a pair of instruments (Beckman Paragon CZE 2000 system), each equipped with seven capillaries. After preliminary (1 month) assessment of possible inter-capillary and inter-instrument variations, the QC material (a home prepared serum pool stored in the frozen state) was assayed daily over a 1-year period. Results: Maximum inter-capillary and inter-instrument differences were 3.1% and 2.4%. No significant trend was observed for daily values (205 measurements over 1 year); in the same period overall imprecision values (CV) were in the interval 1.2% (albumin) to 3.2–6.1% (globulin zones). Mean monthly imprecision (CV) values were in the interval 1.1% (albumin) to 5.2% (globulin zones). There was no significant trend of monthly means with time. The observed imprecision values were within the biological variation-derived goals for imprecision. Conclusions: It is concluded that the assessed analytical instruments, operated in routine conditions, show long-term stability and imprecision consistent with the clinical use of the results produced. [Copyright &y& Elsevier]
Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 9952070
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Precision and long-term stability of capillary electrophoresis measurement of serum protein zones
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  Data: <searchLink fieldCode="AR" term="%22Luraschi%2C+Paola%22">Luraschi, Paola</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Brambilla%2C+Simona%22">Brambilla, Simona</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Infusino%2C+Ilenia%22">Infusino, Ilenia</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Franzini%2C+Carlo%22">Franzini, Carlo</searchLink><relatesTo>2</relatesTo><i> carlo.franzini@unimi.it</i>
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  Data: <searchLink fieldCode="JN" term="%22Clinica+Chimica+Acta%22">Clinica Chimica Acta</searchLink>. Jul2003, Vol. 333 Issue 1, p41. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Electrophoresis%22">Electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+proteins%22">Blood proteins</searchLink>
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  Data: Background: Analytical evaluations of an available system for capillary zone electrophoresis (CZE) of serum protein have been reported. However, data concerning long-term precision and stability of the system, operated under routine conditions, are lacking. We report data from an internal quality control (QC) scheme, obtained over a 1-year period. Methods: Measurements were done with a pair of instruments (Beckman Paragon CZE 2000 system), each equipped with seven capillaries. After preliminary (1 month) assessment of possible inter-capillary and inter-instrument variations, the QC material (a home prepared serum pool stored in the frozen state) was assayed daily over a 1-year period. Results: Maximum inter-capillary and inter-instrument differences were 3.1% and 2.4%. No significant trend was observed for daily values (205 measurements over 1 year); in the same period overall imprecision values (CV) were in the interval 1.2% (albumin) to 3.2–6.1% (globulin zones). Mean monthly imprecision (CV) values were in the interval 1.1% (albumin) to 5.2% (globulin zones). There was no significant trend of monthly means with time. The observed imprecision values were within the biological variation-derived goals for imprecision. Conclusions: It is concluded that the assessed analytical instruments, operated in routine conditions, show long-term stability and imprecision consistent with the clinical use of the results produced. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/S0009-8981(03)00163-3
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      – Code: eng
        Text: English
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        PageCount: 5
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              M: 07
              Text: Jul2003
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              Y: 2003
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