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Breaking the Habit: Isolating Nicotine-Degrading Bacteria in Undergraduate Microbiology Teaching Labs

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Title: Breaking the Habit: Isolating Nicotine-Degrading Bacteria in Undergraduate Microbiology Teaching Labs
Language: English
Authors: J. Mastenbrook, E. Pathak, C. Beaver, F. Stull, B. J. Koestler (ORCID 0000-0001-7213-0953)
Source: Journal of Microbiology & Biology Education. 2024 25(2).
Availability: American Society for Microbiology. 1752 N Street NW, Washington, DC 20036. Tel: 202-737-3600; e-mail: journals@asmusa.org; Web site: https://journals.asm.org/journal/jmbe
Peer Reviewed: Y
Page Count: 5
Publication Date: 2024
Sponsoring Agency: National Institute of General Medical Sciences (NIGMS) (DHHS/NIH)
National Science Foundation (NSF), Division of Chemistry (CHE)
Contract Number: R15GM139069
2236541
Document Type: Journal Articles
Reports - Research
Education Level: Higher Education
Postsecondary Education
Descriptors: Undergraduate Study, Microbiology, Science Activities, Theory Practice Relationship, Public Health, Learner Engagement, Smoking
ISSN: 1935-7877
1935-7885
Abstract: Nicotine is a major alkaloid in tobacco plants and an addictive component of tobacco products. Some bacteria grow on tobacco plants and have evolved the ability to metabolize nicotine. As part of our microbiology teaching lab, we used minimal media with nicotine as the sole carbon source to isolate nicotine-degrading bacteria from tobacco leaves and commercial tobacco products. Students then identified these bacteria using 16S rRNA sequencing and biochemical assays and assessed their ability to catabolize nicotine using UV spectroscopy. Students were able to isolate and identify 14 distinct genera that can metabolize nicotine. This modification of the commonly used unknown project gave students firsthand experience using selective media, and students got the opportunity to work with largely uncharacterized microbes with a real-world connection to public health, which increased student engagement. Students had the opportunity to think critically about why nicotine-degrading microorganisms associate with tobacco plants, why there are different bacteria that use the same specialized metabolism, and how these organisms are isolated from other bacteria using selective media.
Abstractor: As Provided
Entry Date: 2024
Accession Number: EJ1437594
Database: ERIC
FullText Text:
  Availability: 0
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  Data: <searchLink fieldCode="AR" term="%22J%2E+Mastenbrook%22">J. Mastenbrook</searchLink><br /><searchLink fieldCode="AR" term="%22E%2E+Pathak%22">E. Pathak</searchLink><br /><searchLink fieldCode="AR" term="%22C%2E+Beaver%22">C. Beaver</searchLink><br /><searchLink fieldCode="AR" term="%22F%2E+Stull%22">F. Stull</searchLink><br /><searchLink fieldCode="AR" term="%22B%2E+J%2E+Koestler%22">B. J. Koestler</searchLink> (ORCID <externalLink term="http://orcid.org/0000-0001-7213-0953">0000-0001-7213-0953</externalLink>)
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  Data: American Society for Microbiology. 1752 N Street NW, Washington, DC 20036. Tel: 202-737-3600; e-mail: journals@asmusa.org; Web site: https://journals.asm.org/journal/jmbe
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  Data: Nicotine is a major alkaloid in tobacco plants and an addictive component of tobacco products. Some bacteria grow on tobacco plants and have evolved the ability to metabolize nicotine. As part of our microbiology teaching lab, we used minimal media with nicotine as the sole carbon source to isolate nicotine-degrading bacteria from tobacco leaves and commercial tobacco products. Students then identified these bacteria using 16S rRNA sequencing and biochemical assays and assessed their ability to catabolize nicotine using UV spectroscopy. Students were able to isolate and identify 14 distinct genera that can metabolize nicotine. This modification of the commonly used unknown project gave students firsthand experience using selective media, and students got the opportunity to work with largely uncharacterized microbes with a real-world connection to public health, which increased student engagement. Students had the opportunity to think critically about why nicotine-degrading microorganisms associate with tobacco plants, why there are different bacteria that use the same specialized metabolism, and how these organisms are isolated from other bacteria using selective media.
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  Data: 2024
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      – Text: English
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    Subjects:
      – SubjectFull: Undergraduate Study
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      – SubjectFull: Microbiology
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      – SubjectFull: Science Activities
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      – SubjectFull: Theory Practice Relationship
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      – SubjectFull: Public Health
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      – SubjectFull: Learner Engagement
        Type: general
      – SubjectFull: Smoking
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      – TitleFull: Breaking the Habit: Isolating Nicotine-Degrading Bacteria in Undergraduate Microbiology Teaching Labs
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