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Exploration of a Novel Nitrite Detection Method in Teaching: Integrating Fluorescence and Colorimetric Sensing into a Comprehensive Laboratory Module

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Title: Exploration of a Novel Nitrite Detection Method in Teaching: Integrating Fluorescence and Colorimetric Sensing into a Comprehensive Laboratory Module
Language: English
Authors: Nan Wang, Kang-Kang Yu, Qian Zhou (ORCID 0000-0003-0877-2585), Yan-Hong Liu, Xiao-Qi Yu (ORCID 0000-0003-1719-6137), Kun Li (ORCID 0000-0002-8788-1036)
Source: Journal of Chemical Education. 2025 102(12):5324-5330.
Availability: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Peer Reviewed: Y
Page Count: 7
Publication Date: 2025
Document Type: Journal Articles
Reports - Descriptive
Education Level: Higher Education
Postsecondary Education
Descriptors: Science Instruction, Science Laboratories, Undergraduate Study, College Science, Hands on Science, Spectroscopy, Scientific Concepts, Concept Formation
DOI: 10.1021/acs.jchemed.5c01224
ISSN: 0021-9584
1938-1328
Abstract: This work presents a modernized lab module for nitrite analysis using a novel probe, AAC (4-(2-aminophenyl)-7-(diethylamino)-2H-chromen-2-one, C[subscript 19]H[subscript 20]N[subscript 2]O[subscript 2]), offering a safer and more sensitive alternative derived from recent research. The 12-h module follows a three-stage structure (Basic Theory--Method Validation--Comprehensive Application) that emphasizes hands-on problem-solving, method comparison, and solid-sample analysis via fluorescence spectroscopy. By adapting an advanced sensing strategy for undergraduate learning, this module strengthens critical thinking, engagement, and practical skills, effectively bridging innovative research with laboratory education.
Abstractor: As Provided
Entry Date: 2026
Accession Number: EJ1491084
Database: ERIC
FullText Text:
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PubType: Academic Journal
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  Data: Exploration of a Novel Nitrite Detection Method in Teaching: Integrating Fluorescence and Colorimetric Sensing into a Comprehensive Laboratory Module
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  Data: English
– Name: Author
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  Data: <searchLink fieldCode="AR" term="%22Nan+Wang%22">Nan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Kang-Kang+Yu%22">Kang-Kang Yu</searchLink><br /><searchLink fieldCode="AR" term="%22Qian+Zhou%22">Qian Zhou</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0003-0877-2585">0000-0003-0877-2585</externalLink>)<br /><searchLink fieldCode="AR" term="%22Yan-Hong+Liu%22">Yan-Hong Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Xiao-Qi+Yu%22">Xiao-Qi Yu</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0003-1719-6137">0000-0003-1719-6137</externalLink>)<br /><searchLink fieldCode="AR" term="%22Kun+Li%22">Kun Li</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0002-8788-1036">0000-0002-8788-1036</externalLink>)
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  Data: <searchLink fieldCode="SO" term="%22Journal+of+Chemical+Education%22"><i>Journal of Chemical Education</i></searchLink>. 2025 102(12):5324-5330.
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  Data: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
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  Data: Y
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  Data: 7
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  Label: Publication Date
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  Data: 2025
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  Data: Journal Articles<br />Reports - Descriptive
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  Data: <searchLink fieldCode="EL" term="%22Higher+Education%22">Higher Education</searchLink><br /><searchLink fieldCode="EL" term="%22Postsecondary+Education%22">Postsecondary Education</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Science+Instruction%22">Science Instruction</searchLink><br /><searchLink fieldCode="DE" term="%22Science+Laboratories%22">Science Laboratories</searchLink><br /><searchLink fieldCode="DE" term="%22Undergraduate+Study%22">Undergraduate Study</searchLink><br /><searchLink fieldCode="DE" term="%22College+Science%22">College Science</searchLink><br /><searchLink fieldCode="DE" term="%22Hands+on+Science%22">Hands on Science</searchLink><br /><searchLink fieldCode="DE" term="%22Spectroscopy%22">Spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Scientific+Concepts%22">Scientific Concepts</searchLink><br /><searchLink fieldCode="DE" term="%22Concept+Formation%22">Concept Formation</searchLink>
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  Data: 10.1021/acs.jchemed.5c01224
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  Label: ISSN
  Group: ISSN
  Data: 0021-9584<br />1938-1328
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This work presents a modernized lab module for nitrite analysis using a novel probe, AAC (4-(2-aminophenyl)-7-(diethylamino)-2H-chromen-2-one, C[subscript 19]H[subscript 20]N[subscript 2]O[subscript 2]), offering a safer and more sensitive alternative derived from recent research. The 12-h module follows a three-stage structure (Basic Theory--Method Validation--Comprehensive Application) that emphasizes hands-on problem-solving, method comparison, and solid-sample analysis via fluorescence spectroscopy. By adapting an advanced sensing strategy for undergraduate learning, this module strengthens critical thinking, engagement, and practical skills, effectively bridging innovative research with laboratory education.
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  Data: As Provided
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  Label: Entry Date
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  Data: 2026
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  Label: Accession Number
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  Data: EJ1491084
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      – Text: English
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    Subjects:
      – SubjectFull: Science Instruction
        Type: general
      – SubjectFull: Science Laboratories
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      – SubjectFull: Undergraduate Study
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      – SubjectFull: Concept Formation
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      – TitleFull: Exploration of a Novel Nitrite Detection Method in Teaching: Integrating Fluorescence and Colorimetric Sensing into a Comprehensive Laboratory Module
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            NameFull: Nan Wang
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            NameFull: Kun Li
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