LA EXPOSICIÓN PRENATAL A FLUORURO INDUCE CAMBIOS EN LA PORCIÓN CORONAL DE LA CRIPTA ÓSEA Y ALTERA LA ERUPCIÓN DENTAL EN CRÍAS LACTANTES.
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| Title: | LA EXPOSICIÓN PRENATAL A FLUORURO INDUCE CAMBIOS EN LA PORCIÓN CORONAL DE LA CRIPTA ÓSEA Y ALTERA LA ERUPCIÓN DENTAL EN CRÍAS LACTANTES. |
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| Alternate Title: | PRENATAL FLUORIDE EXPOSURE INDUCES CHANGES IN THE CORONAL PORTION OF BONY CRYPT AND ALTERS DENTAL ERUPTION IN SUCKLING RATS. |
| Authors: | Interlandi, Victoria1, Fontanetti, Pablo1, Delgado, Andrea1, Ponce, Rubén1, Gallará, Raquel1, Centeno, Viviana1 centenovivi@yahoo.es |
| Source: | Actualizaciones en Osteología. Ene-Apr2020, Vol. 16 Issue 1, p35-46. 12p. |
| Subjects: | TOOTH eruption, BONES, CONTROL groups, SODIUM fluoride, LACTATION, CANCELLOUS bone, MANDIBLE |
| Abstract (English): | Tooth eruption is a tightly regulated and spatially and temporally programmed process. The aim of this study was to examine the effect of prenatal NaF exposure on the morphological and cellular events that occur in the supracoronal area of bony crypt of the first rat molar during the pre-eruptive stage. Offspring from two groups of rats were used (6-8 per group): Control and 50 mg/L NaF. The treatment was performed during pregnancy and lactation. Suckling pups were euthanized at 3-, 10- and 15-days-old by cervical dislocation. Mandibles were removed and histologically processed to obtain buccolingual sections stained with H&E. In sections of first mandibular molar of 3- and 10-days-old pups, the following static histomorphometric parameters were evaluated: trabecular bone volume [BV/TV (%)] and number of osteoclasts (N.Oc/mm). Also, indirect parameters were obtained: trabecular number [Tb.N (1/mm)], trabecular thickness [Tb.Th (µm)], and trabecular separation [Tb.Sp (µm)]. The degree of tooth eruption [TED (µm)] was determined. Results are expressed as mean ± SE and analyzed by Student t-test. Histomorphometric analysis showed an increase in the BV/TV (%) of the bone crypt of 3- and 10- days-old pups exposed to NaF (p <0.01); this increase was associated with a decrease in the N.Oc/mm (p <0.01). TED of mandibular first molar was lower in prenatal NaF exposed group than in control group (p<0.01). In conclusion, the increased BV/TV and the lower N.Oc observed in the bone crypt of 3- and 10- days-old pups from mothers treated with NaF suggested a disruptive effect triggered by F- on the formation events of the eruptive pathway in the offspring. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | La erupción dental es un proceso estrictamente regulado y programado espacial y temporalmente. El objetivo del trabajo fue estudiar el efecto de la exposición prenatal a fluoruro de sodio (NaF) sobre los eventos morfológicos y celulares que ocurren en el hueso supracoronal del primer molar de crías de rata durante la etapa preeruptiva. Se emplearon crías (n=6-8 por grupo) provenientes de madres que bebieron crónicamente agua con diferentes concentraciones de F- en forma de NaF durante la gestación y lactancia: control y NaF (50 mg/L). En cortes histológicos de la mandíbula de crías de 3 y 10 días se analizaron parámetros de histomorfometría estática en la zona supracoronal de la canastilla ósea a la altura del primer molar inferior: volumen óseo trabecular [BV/TV (%)], número de osteoclastos por milímetro (N.Oc/mm) y las variables indirectas: número de trabéculas [Tb.N (1/mm)], espesor [Tb.Th (µm)] y separación trabecular [Tb.Sp (µm)]. En crías de 15 días se midió el grado de erupción [TED (µm)] del primer molar inferior. Los resultados se analizaron con el test "t" de Student considerando diferencias significativas a p<0,05. El análisis histomorfométrico demostró un incremento en el BV/TV (%) del hueso supracoronal (p<0,01) asociado con disminución del N.Oc/mm (p<0,01) en crías de 3 y 10 días expuestas prenatalmente al F-. El grado de erupción dental fue menor en animales expuestos prenatalmente al F- en comparación con los controles (p<0,01). En conclusión, los resultados observados en la mandíbula de crías expuestas durante la etapa prenatal y posnatal temprana al F- sugieren un efecto disruptivo sobre la actividad resortiva necesaria para formación del canal eruptivo. [ABSTRACT FROM AUTHOR] |
| Copyright of Actualizaciones en Osteología is the property of Asociacion Argentina de Osteologia y Metabolismo Mineral and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 145388804 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: LA EXPOSICIÓN PRENATAL A FLUORURO INDUCE CAMBIOS EN LA PORCIÓN CORONAL DE LA CRIPTA ÓSEA Y ALTERA LA ERUPCIÓN DENTAL EN CRÍAS LACTANTES. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: PRENATAL FLUORIDE EXPOSURE INDUCES CHANGES IN THE CORONAL PORTION OF BONY CRYPT AND ALTERS DENTAL ERUPTION IN SUCKLING RATS. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Interlandi%2C+Victoria%22">Interlandi, Victoria</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fontanetti%2C+Pablo%22">Fontanetti, Pablo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Delgado%2C+Andrea%22">Delgado, Andrea</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ponce%2C+Rubén%22">Ponce, Rubén</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gallará%2C+Raquel%22">Gallará, Raquel</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Centeno%2C+Viviana%22">Centeno, Viviana</searchLink><relatesTo>1</relatesTo><i> centenovivi@yahoo.es</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Actualizaciones+en+Osteología%22">Actualizaciones en Osteología</searchLink>. Ene-Apr2020, Vol. 16 Issue 1, p35-46. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22TOOTH+eruption%22">TOOTH eruption</searchLink><br /><searchLink fieldCode="DE" term="%22BONES%22">BONES</searchLink><br /><searchLink fieldCode="DE" term="%22CONTROL+groups%22">CONTROL groups</searchLink><br /><searchLink fieldCode="DE" term="%22SODIUM+fluoride%22">SODIUM fluoride</searchLink><br /><searchLink fieldCode="DE" term="%22LACTATION%22">LACTATION</searchLink><br /><searchLink fieldCode="DE" term="%22CANCELLOUS+bone%22">CANCELLOUS bone</searchLink><br /><searchLink fieldCode="DE" term="%22MANDIBLE%22">MANDIBLE</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Tooth eruption is a tightly regulated and spatially and temporally programmed process. The aim of this study was to examine the effect of prenatal NaF exposure on the morphological and cellular events that occur in the supracoronal area of bony crypt of the first rat molar during the pre-eruptive stage. Offspring from two groups of rats were used (6-8 per group): Control and 50 mg/L NaF. The treatment was performed during pregnancy and lactation. Suckling pups were euthanized at 3-, 10- and 15-days-old by cervical dislocation. Mandibles were removed and histologically processed to obtain buccolingual sections stained with H&E. In sections of first mandibular molar of 3- and 10-days-old pups, the following static histomorphometric parameters were evaluated: trabecular bone volume [BV/TV (%)] and number of osteoclasts (N.Oc/mm). Also, indirect parameters were obtained: trabecular number [Tb.N (1/mm)], trabecular thickness [Tb.Th (µm)], and trabecular separation [Tb.Sp (µm)]. The degree of tooth eruption [TED (µm)] was determined. Results are expressed as mean ± SE and analyzed by Student t-test. Histomorphometric analysis showed an increase in the BV/TV (%) of the bone crypt of 3- and 10- days-old pups exposed to NaF (p <0.01); this increase was associated with a decrease in the N.Oc/mm (p <0.01). TED of mandibular first molar was lower in prenatal NaF exposed group than in control group (p<0.01). In conclusion, the increased BV/TV and the lower N.Oc observed in the bone crypt of 3- and 10- days-old pups from mothers treated with NaF suggested a disruptive effect triggered by F- on the formation events of the eruptive pathway in the offspring. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: La erupción dental es un proceso estrictamente regulado y programado espacial y temporalmente. El objetivo del trabajo fue estudiar el efecto de la exposición prenatal a fluoruro de sodio (NaF) sobre los eventos morfológicos y celulares que ocurren en el hueso supracoronal del primer molar de crías de rata durante la etapa preeruptiva. Se emplearon crías (n=6-8 por grupo) provenientes de madres que bebieron crónicamente agua con diferentes concentraciones de F- en forma de NaF durante la gestación y lactancia: control y NaF (50 mg/L). En cortes histológicos de la mandíbula de crías de 3 y 10 días se analizaron parámetros de histomorfometría estática en la zona supracoronal de la canastilla ósea a la altura del primer molar inferior: volumen óseo trabecular [BV/TV (%)], número de osteoclastos por milímetro (N.Oc/mm) y las variables indirectas: número de trabéculas [Tb.N (1/mm)], espesor [Tb.Th (µm)] y separación trabecular [Tb.Sp (µm)]. En crías de 15 días se midió el grado de erupción [TED (µm)] del primer molar inferior. Los resultados se analizaron con el test "t" de Student considerando diferencias significativas a p<0,05. El análisis histomorfométrico demostró un incremento en el BV/TV (%) del hueso supracoronal (p<0,01) asociado con disminución del N.Oc/mm (p<0,01) en crías de 3 y 10 días expuestas prenatalmente al F-. El grado de erupción dental fue menor en animales expuestos prenatalmente al F- en comparación con los controles (p<0,01). En conclusión, los resultados observados en la mandíbula de crías expuestas durante la etapa prenatal y posnatal temprana al F- sugieren un efecto disruptivo sobre la actividad resortiva necesaria para formación del canal eruptivo. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Actualizaciones en Osteología is the property of Asociacion Argentina de Osteologia y Metabolismo Mineral and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=145388804 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 12 StartPage: 35 Subjects: – SubjectFull: TOOTH eruption Type: general – SubjectFull: BONES Type: general – SubjectFull: CONTROL groups Type: general – SubjectFull: SODIUM fluoride Type: general – SubjectFull: LACTATION Type: general – SubjectFull: CANCELLOUS bone Type: general – SubjectFull: MANDIBLE Type: general Titles: – TitleFull: LA EXPOSICIÓN PRENATAL A FLUORURO INDUCE CAMBIOS EN LA PORCIÓN CORONAL DE LA CRIPTA ÓSEA Y ALTERA LA ERUPCIÓN DENTAL EN CRÍAS LACTANTES. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Interlandi, Victoria – PersonEntity: Name: NameFull: Fontanetti, Pablo – PersonEntity: Name: NameFull: Delgado, Andrea – PersonEntity: Name: NameFull: Ponce, Rubén – PersonEntity: Name: NameFull: Gallará, Raquel – PersonEntity: Name: NameFull: Centeno, Viviana IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Ene-Apr2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 16698975 Numbering: – Type: volume Value: 16 – Type: issue Value: 1 Titles: – TitleFull: Actualizaciones en Osteología Type: main |
| ResultId | 1 |
