Comparative genetic, biochemical, and biophysical analyses of the four E. coli ABCF paralogs support distinct functions related to mRNA translation. - Ousalem F; Singh S; Bailey NA; Wong KH; Zhu L; Neky MJ; Sibindi C; Fei J; Gonzalez RL; Boël G; Hunt JF (BioRxiv : the preprint server for biology, 2023)