View in EDS

A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.

Saved in:
Bibliographic Details
Title: A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
Authors: López-Romero EO; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Arévalo-Salina EL; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Arcos-Hernández C; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Sánchez-Guevara Y; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Beltrán C; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Saab-Rincón G; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico., Nishigaki T; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico. Electronic address: takuya.nishigaki@ibt.unam.mx.
Source: Protein expression and purification [Protein Expr Purif] 2025 May; Vol. 229, pp. 106667. Date of Electronic Publication: 2025 Jan 30.
Publication Type: Journal Article
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE
Database: MEDLINE Ultimate
FullText Text:
  Availability: 0
Header DbId: mdl
DbLabel: MEDLINE Ultimate
An: 39892532
AccessLevel: 2
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22López-Romero+EO%22">López-Romero EO</searchLink>; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Arévalo-Salina+EL%22">Arévalo-Salina EL</searchLink>; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Arcos-Hernández+C%22">Arcos-Hernández C</searchLink>; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Sánchez-Guevara+Y%22">Sánchez-Guevara Y</searchLink>; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Beltrán+C%22">Beltrán C</searchLink>; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Saab-Rincón+G%22">Saab-Rincón G</searchLink>; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.<br /><searchLink fieldCode="AU" term="%22Nishigaki+T%22">Nishigaki T</searchLink>; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico. Electronic address: takuya.nishigaki@ibt.unam.mx.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%229101496%22">Protein expression and purification</searchLink> [Protein Expr Purif] 2025 May; Vol. 229, pp. 106667. <i>Date of Electronic Publication: </i>2025 Jan 30.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Academic+Press%22">Academic Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>9101496 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1096-0279 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2210465928%22">10465928 </searchLink><i>NLM ISO Abbreviation: </i>Protein Expr Purif <i>Subsets: </i>MEDLINE
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdl&AN=39892532
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.pep.2025.106667
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 106667
    Titles:
      – TitleFull: A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: López-Romero EO
      – PersonEntity:
          Name:
            NameFull: Arévalo-Salina EL
      – PersonEntity:
          Name:
            NameFull: Arcos-Hernández C
      – PersonEntity:
          Name:
            NameFull: Sánchez-Guevara Y
      – PersonEntity:
          Name:
            NameFull: Beltrán C
      – PersonEntity:
          Name:
            NameFull: Saab-Rincón G
      – PersonEntity:
          Name:
            NameFull: Nishigaki T
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Text: 2025 May
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-electronic
              Value: 1096-0279
          Numbering:
            – Type: volume
              Value: 229
          Titles:
            – TitleFull: Protein expression and purification
              Type: main
ResultId 1