A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol. - López-Romero EO; Arévalo-Salina EL; Arcos-Hernández C; Sánchez-Guevara Y; Beltrán C; Saab-Rincón G; Nishigaki T (Protein expression and purification, 2025)