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The unresolved struggle of 16S rRNA amplicon sequencing: a benchmarking analysis of clustering and denoising methods.

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Title: The unresolved struggle of 16S rRNA amplicon sequencing: a benchmarking analysis of clustering and denoising methods.
Authors: Fares M; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt.; Veterinary Research Institute, National Research Centre, Giza, Egypt., Tharwat EK; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt., Cleenwerck I; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium., Monsieurs P; Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium., Van Houdt R; Microbiology Unit, Nuclear Medical Applications, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium., Vandamme P; Laboratory for Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium., El-Hadidi M; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt., Mysara M; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt. MMaysara@nu.edu.eg.
Source: Environmental microbiome [Environ Microbiome] 2025 May 13; Vol. 20 (1), pp. 51. Date of Electronic Publication: 2025 May 13.
Publication Type: Journal Article
Journal Info: Publisher: BMC Country of Publication: England NLM ID: 101768168 Publication Model: Electronic Cited Medium: Internet ISSN: 2524-6372 (Electronic) Linking ISSN: 25246372 NLM ISO Abbreviation: Environ Microbiome Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Fares+M%22">Fares M</searchLink>; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt.; Veterinary Research Institute, National Research Centre, Giza, Egypt.<br /><searchLink fieldCode="AU" term="%22Tharwat+EK%22">Tharwat EK</searchLink>; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt.<br /><searchLink fieldCode="AU" term="%22Cleenwerck+I%22">Cleenwerck I</searchLink>; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.<br /><searchLink fieldCode="AU" term="%22Monsieurs+P%22">Monsieurs P</searchLink>; Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Van+Houdt+R%22">Van Houdt R</searchLink>; Microbiology Unit, Nuclear Medical Applications, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium.<br /><searchLink fieldCode="AU" term="%22Vandamme+P%22">Vandamme P</searchLink>; Laboratory for Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.<br /><searchLink fieldCode="AU" term="%22El-Hadidi+M%22">El-Hadidi M</searchLink>; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt.<br /><searchLink fieldCode="AU" term="%22Mysara+M%22">Mysara M</searchLink>; Bioinformatics Group, Center for Informatics Science, School of Information Technology and Computer Science, Nile University, Giza, Egypt. MMaysara@nu.edu.eg.
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              Text: 2025 May 13
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