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DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma.

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Title: DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma.
Authors: Surwase SS; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University., Zhou XMM; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Dermatology, Johns Hopkins University., Luly KM; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University., Zhu Q; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Convergence Institute, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine., Talebian N; Department of Pathology, Johns Hopkins University., Anders RA; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Convergence Institute, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine., Green JJ; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Oncology, Johns Hopkins University; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Institute for Nanobiotechnology, Johns Hopkins University; Departments of Neurosurgery and Ophthalmology, Johns Hopkins University School of Medicine; Departments of Materials Science & Engineering and Chemical & Biomolecular Engineering, Johns Hopkins University., Tzeng SY; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University., Sunshine JC; Department of Biomedical Engineering, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Dermatology, Johns Hopkins University; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; joelsunshine@jhmi.edu.
Source: Journal of visualized experiments : JoVE [J Vis Exp] 2025 Jun 06 (220). Date of Electronic Publication: 2025 Jun 06.
Publication Type: Journal Article; Video-Audio Media; Research Support, N.I.H., Extramural
Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma.
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  Data: <searchLink fieldCode="AU" term="%22Surwase+SS%22">Surwase SS</searchLink>; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Zhou+XMM%22">Zhou XMM</searchLink>; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Dermatology, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Luly+KM%22">Luly KM</searchLink>; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Zhu+Q%22">Zhu Q</searchLink>; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Convergence Institute, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine.<br /><searchLink fieldCode="AU" term="%22Talebian+N%22">Talebian N</searchLink>; Department of Pathology, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Anders+RA%22">Anders RA</searchLink>; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Convergence Institute, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine.<br /><searchLink fieldCode="AU" term="%22Green+JJ%22">Green JJ</searchLink>; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Oncology, Johns Hopkins University; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Institute for Nanobiotechnology, Johns Hopkins University; Departments of Neurosurgery and Ophthalmology, Johns Hopkins University School of Medicine; Departments of Materials Science & Engineering and Chemical & Biomolecular Engineering, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Tzeng+SY%22">Tzeng SY</searchLink>; Department of Biomedical Engineering, Johns Hopkins University; Translational Tissue Engineering Center, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University.<br /><searchLink fieldCode="AU" term="%22Sunshine+JC%22">Sunshine JC</searchLink>; Department of Biomedical Engineering, Johns Hopkins University; Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University; Department of Dermatology, Johns Hopkins University; Department of Oncology, Johns Hopkins University; Department of Pathology, Johns Hopkins University; Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine; joelsunshine@jhmi.edu.
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        Value: 10.3791/68124
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              Text: 2025 Jun 06
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