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Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics.

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Title: Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics.
Authors: Zizmare L; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany., Hofmann U; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany., Jarboui MA; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany., Klose F; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany., Fraschka S; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany., Matthes J; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany., Krüger M; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany., Schaeffeler E; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany., Schwab M; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany.; Department of Clinical Pharmacology, and Department of Biochemistry and Pharmacy, University of Tübingen, 72076, Tübingen, Germany., Ueffing M; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany., Pichler BJ; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany., Boldt K; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany., Casadei N; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany., Trautwein C; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.; Core Facility Metabolomics, Faculty of Medicine, University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.
Source: Scientific reports [Sci Rep] 2025 Jun 23; Vol. 15 (1), pp. 20254. Date of Electronic Publication: 2025 Jun 23.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics.
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  Data: <searchLink fieldCode="AU" term="%22Zizmare+L%22">Zizmare L</searchLink>; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Hofmann+U%22">Hofmann U</searchLink>; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany.<br /><searchLink fieldCode="AU" term="%22Jarboui+MA%22">Jarboui MA</searchLink>; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Klose+F%22">Klose F</searchLink>; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Fraschka+S%22">Fraschka S</searchLink>; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Matthes+J%22">Matthes J</searchLink>; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Krüger+M%22">Krüger M</searchLink>; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Schaeffeler+E%22">Schaeffeler E</searchLink>; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany.<br /><searchLink fieldCode="AU" term="%22Schwab+M%22">Schwab M</searchLink>; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.; Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, University of Tübingen, 70376, Stuttgart, Germany.; Department of Clinical Pharmacology, and Department of Biochemistry and Pharmacy, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Ueffing+M%22">Ueffing M</searchLink>; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Pichler+BJ%22">Pichler BJ</searchLink>; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Boldt+K%22">Boldt K</searchLink>; Core Facility for Medical Proteomics, Institute for Ophthalmic Research, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Casadei+N%22">Casadei N</searchLink>; NGS Competence Center Tübingen (NCCT), Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076, Tübingen, Germany.<br /><searchLink fieldCode="AU" term="%22Trautwein+C%22">Trautwein C</searchLink>; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.; Cluster of Excellence iFIT (EXC2180) 'Image-Guided and Functionally Instructed Tumor Therapies', University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.; Core Facility Metabolomics, Faculty of Medicine, University of Tübingen, 72076, Tübingen, Germany. Christoph.Trautwein@med.uni-tuebingen.de.
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