DNA reference reagents isolate biases in microbiome profiling: a global multi-lab study.
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| Title: | DNA reference reagents isolate biases in microbiome profiling: a global multi-lab study. |
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| Authors: | Anwar S; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Lamaudiere M; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Hassall J; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Dehinsilu J; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Bhuller RK; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Hold GL; UNSW Microbiome Research Centre, St George Hospital, Kogarah, New South Wales, Australia., Vázquez-Campos X; NSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia., Mahnert A; Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria., Moissl-Eichinger C; Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.; BioTechMed-Graz, Graz, Austria., Gallé B; Core Facility Molecular Biology, Center for medical research, Medical University of Graz, Graz, Austria., Kainz G; Core Facility Molecular Biology, Center for medical research, Medical University of Graz, Graz, Austria., Pjevac P; Joint Microbiome Facility of the Medical University of Vienna, University of Vienna, Vienna, Austria.; Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, UBB University of Vienna, Vienna, Austria., Hausmann B; Joint Microbiome Facility of the Medical University of Vienna, University of Vienna, Vienna, Austria.; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Schwarz J; Joint Microbiome Facility of the Medical University of Vienna, University of Vienna, Vienna, Austria.; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Kohl G; Joint Microbiome Facility of the Medical University of Vienna, University of Vienna, Vienna, Austria.; Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, UBB University of Vienna, Vienna, Austria., Berry D; Joint Microbiome Facility of the Medical University of Vienna, University of Vienna, Vienna, Austria.; Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, UBB University of Vienna, Vienna, Austria., Vancuren SJ; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada., Allen-Vercoe E; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada., Nielsen N; Clinical Microbiomics A/S, Copenhagen, Denmark., Sørensen N; Clinical Microbiomics A/S, Copenhagen, Denmark., Eklund A; Clinical Microbiomics A/S, Copenhagen, Denmark., Nielsen HB; Clinical Microbiomics A/S, Copenhagen, Denmark., Riedel R; German Rheumatology Research Center Berlin, A Leibniz Institute, Berlin, Germany.; Bioinformatics and Computational Biology, University Heart & Vascular Centre Hamburg, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany., Krause JL; German Rheumatology Research Center Berlin, A Leibniz Institute, Berlin, Germany., Chang H-D; German Rheumatology Research Center Berlin, A Leibniz Institute, Berlin, Germany.; Chair of Cytometry, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany., Park S; Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan-si, South Korea.; BiowaveW, Seoul, South Korea., Song H-Y; Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan-si, South Korea., Seo H; Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan-si, South Korea., Ul-Haq A; Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan-si, South Korea., Kim S; Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan-si, South Korea., Kwon Y; BiowaveW, Seoul, South Korea., Park S; BiowaveW, Seoul, South Korea.; Department of Obstetrics and Gynecology, Ewha College of Medicine, Seoul, South Korea., Soberon X; Unidad de Vinculación Cientifica UNAM-INMEGEN, Instituto Nacional de Medicina Genomica, Mexico, Mexico.; Instituto de Biotecnologia UNAM, Cuernavaca, Morelos, Mexico., Silva-Herzog E; Unidad de Vinculación Cientifica UNAM-INMEGEN, Instituto Nacional de Medicina Genomica, Mexico, Mexico.; Instituto de Biotecnologia UNAM, Cuernavaca, Morelos, Mexico., Verlouw JAM; Laboratory of Population Genomics, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Arp P; Laboratory of Population Genomics, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Jhamai M; Laboratory of Population Genomics, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Kraaij R; Laboratory of Population Genomics, Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Geelen AR; Center for Microbiome Analyses and Therapeutics, Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands., Ducarmon QR; Center for Microbiome Analyses and Therapeutics, Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands., Smits WK; Center for Microbiome Analyses and Therapeutics, Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands., Kuijper EJ; Center for Microbiome Analyses and Therapeutics, Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands., Zwittink RD; Center for Microbiome Analyses and Therapeutics, Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands., van Best N; Department of Medical Microbiology, Infectious Diseases and Infection Prevention, School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, the Netherlands., Penders J; Department of Medical Microbiology, Infectious Diseases and Infection Prevention, School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, the Netherlands., Le G; Department of Medical Microbiology, Infectious Diseases and Infection Prevention, School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, the Netherlands., Driessen C; Department of Medical Microbiology, Infectious Diseases and Infection Prevention, School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, the Netherlands., Kool J; Center for infectious disease control, Dutch National Institute for Public Health and the Environment, Bilthoven, the Netherlands., Shetty SA; Center for infectious disease control, Dutch National Institute for Public Health and the Environment, Bilthoven, the Netherlands., Fuentes S; Center for infectious disease control, Dutch National Institute for Public Health and the Environment, Bilthoven, the Netherlands., Demirci M; Department of Medical Microbiology, Faculty of Medicine, Kırklareli University, Kırklareli, Turkey., Yigin A; Department of Genetics, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey., Whalley C; Genomics Birmingham, Institute of Cancer & Genomic Sciences, University of Birmingham, Birmingham, United Kingdom., Beggs AD; Genomics Birmingham, Institute of Cancer & Genomic Sciences, University of Birmingham, Birmingham, United Kingdom., Quince C; Earlham Institute and Quadram Institute Bioscience, Norwich, United Kingdom., James R; Earlham Institute and Quadram Institute Bioscience, Norwich, United Kingdom., Raguideau S; Earlham Institute and Quadram Institute Bioscience, Norwich, United Kingdom., Gordon M; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Mate R; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Fritzsche M; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Danckert NP; Imperial College London, London, United Kingdom., Blanco JM; Imperial College London, London, United Kingdom., Marchesi JR; Imperial College London, London, United Kingdom., Rauch M; AlphaBiomics Ltd., London, United Kingdom., Williamson RA; AlphaBiomics Ltd., London, United Kingdom., Van't Wout AB; AlphaBiomics Ltd., London, United Kingdom., Kritz A; Source BioScience UK LTD, Nottingham, United Kingdom., Rosecker S; Source BioScience UK LTD, Nottingham, United Kingdom., Stevens R; Source BioScience UK LTD, Nottingham, United Kingdom., Laws L; Source BioScience UK LTD, Nottingham, United Kingdom., Sayavedra L; Quadram Institute Bioscience-Gut Microbes and Health Research Programme, Norwich, United Kingdom., Romano S; Quadram Institute Bioscience-Gut Microbes and Health Research Programme, Norwich, United Kingdom., Telatin A; Quadram Institute Bioscience-Gut Microbes and Health Research Programme, Norwich, United Kingdom., Baker D; Quadram Institute Bioscience-Gut Microbes and Health Research Programme, Norwich, United Kingdom., Narbad A; Quadram Institute Bioscience-Gut Microbes and Health Research Programme, Norwich, United Kingdom., Servetas SL; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Kralj JG; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Forry SP; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Hunter ME; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Dootz JN; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Jackson SA; National Institute of Standards and Technology, Gaithersburg, Maryland, USA., Mason CE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA., Butler DJ; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA., Mozsary C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA., Foox J; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA., Damle N; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA., Resh A; Diversigen, Inc., New Brighton, Minnesota, USA., Busswitz A; Diversigen, Inc., New Brighton, Minnesota, USA., Lenz P; Diversigen, Inc., New Brighton, Minnesota, USA., Sontag S; Diversigen, Inc., New Brighton, Minnesota, USA., Cross A; Diversigen, Inc., New Brighton, Minnesota, USA., Sanchez C; Diversigen, Inc., New Brighton, Minnesota, USA., Guo M; Diversigen, Inc., New Brighton, Minnesota, USA., Olson K; Diversigen, Inc., New Brighton, Minnesota, USA., Smith EA; Diversigen, Inc., New Brighton, Minnesota, USA., La Reau AJ; Diversigen, Inc., New Brighton, Minnesota, USA., Ward T; Diversigen, Inc., New Brighton, Minnesota, USA., Kuersten S; Illumina, Inc., San Diego, California, USA., Hyde F; Illumina, Inc., San Diego, California, USA., Khrebtukova I; Illumina, Inc., San Diego, California, USA., Schroth G; Illumina, Inc., San Diego, California, USA., Rijpkema S; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Amos GCA; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom., Sergaki C; Medicines and Healthcare Products Regulatory Agency, London, United Kingdom. |
| Source: | MSystems [mSystems] 2025 Nov 18; Vol. 10 (11), pp. e0046625. Date of Electronic Publication: 2025 Oct 17. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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